Effect of intra-articular injection of mesenchymal stem cells in cartilage repair in experimental animals

Kamel, Nadia Salah; Arafa, Mostafa A.; Nadim, Amr; Amer, Hnaa; Amin, Irene Raouf; Samir, Naglaa; Salem, A. Ben

doi:10.1016/j.ejr.2014.03.001

Cited by 9 publications

(4 citation statements)

References 32 publications

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“…In fact, the regeneration ability of cartilage is limited due to its isolation from systemic regulation and its lack of vessels and nerves [3]. Intra-articular injection of Human umbilical cord blood as a new source of mesenchymal stem cells was found effective for cartilage repair in rats with osteoarthritis [4].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of the intra-articular injection of platelet rich plasma in the treatment of patients with primary knee osteoarthritis

Hassan

El-Shafey

Ahmed

et al. 2015

The Egyptian Rheumatologist

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Osteoarthritis (OA) is the most prevalent form of arthritis in the world. Aim of the work: To evaluate the effect of 6-monthly intra-articular injection of platelet rich plasma (PRP) on the functional status of knee joint measured by the International Knee Document Committee scale (IKDC), the visual analogue scale for pain (VAS) on the ultrasonographic findings of OA knee. Assessment of its safety as a new line of treatment was taken into consideration.Patients and methods: The study was carried out on 20 patients with mild to moderate primary knee OA. They were injected intra-articularly with 5 ml PRP for each affected joint, at 1 month intervals for 6 injections. Clinical examination, VAS, IKDC score and diagnostic Doppler ultrasonography were performed before and after PRP treatment.Results: After 6 months of PRP, there was a significant improvement in the duration of inactivity stiffness (8.3 ± 2.4 min), VAS score (3.9 ± 1.1) and IKDC score (74.3 ± 10.2) compared to baseline values (18.7 ± 6.5 min, 5.9 ± 1.3 and 40.9 ± 10.4 respectively; p < 0.001). A significant improvement in Doppler activity (p = 0.04) and synovial thickening (p < 0.001) was found after 6 months of PRP. A significant correlation was found between age of patients, body mass index and disease duration with the VAS (r = 0.55, p < 0.001 and r = 0.29, p = 0.03 and r = 0.71, Please cite this article in press as: Hassan AS et al. Effectiveness of the intra-articular injection of platelet rich plasma in the treatment of patients with primary knee osteoarthritis, The Egyptian Rheumatologist (2014), http://dx.) and a negative correlation with the IKDC score (r = À0.32, p = 0.03, r = À0.96, p < 0.001 and r = À0.79, p < 0.001 respectively). Conclusion: Intra-articular injection of PRP is an effective and safe method for treatment of knee OA. Maximal improvement is obtained in patients with young age and short disease duration.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of the intra-articular injection of platelet rich plasma in the treatment of patients with primary knee osteoarthritis

Hassan

El-Shafey

Ahmed

et al. 2015

The Egyptian Rheumatologist

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“…The minimal cell concentration that MRI can reliably detect using T2∗-weighted sequences is 1 × 10 5 cells/ml/microtube. That has been demonstrated to be sufficient to detect MSCs engraftment in many recent clinical trials involving injection of MSCs at levels greater than 1 × 10 6 cells/ml [ 7 , 8 , 10 , 33 , 34 , 35 , 36 ]. This study did not evaluate artifact effects from the iron particles; additional in vivo studies are needed to confirm and evaluate the quality of the images and any other effects related to iron particle artifacts.…”

Section: Discussionmentioning

confidence: 99%

Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study

Tangchitphisut

Srikaew

Phongkitkarun

et al. 2020

Heliyon

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Objectives The objective of this study was to investigate iron sucrose labeling in mesenchymal stem cell (MSCs) tracking. Background Adipose-derived mesenchymal stem cell-based therapy is a promising strategy for promoting musculoskeletal repair. Methods Iron sucrose-labeled adipose-derived mesenchymal stem cells (IS-labeled ASCs) were tracked using T2-and T2∗-weighted sequences by 1.5 and 3 T MRI in an in vitro model. ASCs were isolated from cosmetic liposuction specimens. ASCs from passages 4–6 were labeled with iron sucrose (Venofer®) which was added to the cell culture medium. Pre- and post-iron sucrose labeled ASCs were evaluated for cell surface immunophenotypes. Cell viability as well as chondrogenic, adipogenic and osteogenic differentiation of IS-labeled-ASCs were evaluated. The IS-labeled ASCs were titrated into microtubes at 1 × 10 3 , 1 × 10 4 , 1 × 10 5 and 1 × 10 6 cells/ml/microtube and their intensities were determined by 1.5 and 3T MRI using T2-and T2∗-weighted sequences. Results The expression markers of IS-labeled ASCs from flow cytometry were equivalent to control. The mean cell viability was 97.73 ± 2.06%. Cell differentiations of IS-labeled ASCs were confirmed in each lineage using specific staining solutions. T2∗-weighted sequences (T2∗) were able to detect iron sucrose labeled-ASCs at a minimum of 1 × 10 5 cells/ml/microtube using 1.5 and 3T MRI, but the detection sensitivity was lower with T2-weighted sequences (T2). Conclusions Iron sucrose incubation is a safe alternative method for ASCs labeling and tracking using MRI following treatment. Clinicians and researchers should be able to visualize the location of ASCs engraftment without secondary surgical investigation involving tissue sampling.

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“…1,2 Many studies show that the use of culture expanded MSC and BMC for the treatment of different orthopedic conditions, for example, tendinopathy, osteonecrosis of the femoral head and especially osteoarthritis and focal cartilage lesions is feasible, safe, and can provide some clinical efficacy in terms of pain reduction, joint function and quality of life. [3][4][5][6][7][8][9][10] Furthermore, in a recent study Cassano et al 11 found a significant concentration of interleukin 1 receptor antagonist (IL-1ra) in BMC while absent in PRP. IL-1ra can inhibit the IL-1 catabolism that could even result in a higher anti-inflammatory potential.…”

Section: Introductionmentioning

confidence: 99%

A Review of Commercially Available Point-of-Care Devices to Concentrate Bone Marrow for the Treatment of Osteoarthritis and Focal Cartilage Lesions

et al. 2018

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Objective Mesenchymal stem cells (MSCs) are a promising cell-based therapy treatment option for several orthopedic indications. Because culture expansion of MSC is time and cost intensive, a bedside concentration of bone marrow (BM) aspirate is used as an alternative. Many commercial systems are available but the available literature and knowledge regarding these systems is limited. We compared different point-of-care devices that concentrate BM (BMC) by focusing on technical features and quality parameters to help surgeons make informed decisions while selecting the appropriate device. Methods We compared published data on the BMC devices of Arteriocyte, Arthrex, Celling Biosciences, EmCyte, Exactech, ISTO Tech, Harvest Tech/Terumo BCT, and Zimmer/BIOMET regarding technical features (centrifugation speed/time, input/output volume, kit components, type of aspiration syringes, filter usage) and quality parameters of their final BMC product (hematocrit, concentration of platelets and total nucleated cells, concentration of MSC and connective tissue progenitor cells). Results The systems differ significantly in their technical features and centrifugation parameters. Only the fully automated systems use universal kits, which allow processing different volumes of BM. Only the Arthrex system allows selection of final hematocrit. There was no standardized reporting method to describe biologic potency. Conclusions Based on the data obtained in this review, recommending a single device is not possible because the reported data could not be compared between devices. A standardized reporting method is needed for valid comparisons. Furthermore, clinical outcomes are required to establish the true efficacy of these systems. We are conducting additional studies for more careful comparison among the devices.

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Effect of intra-articular injection of mesenchymal stem cells in cartilage repair in experimental animals

Cited by 9 publications

References 32 publications

Effectiveness of the intra-articular injection of platelet rich plasma in the treatment of patients with primary knee osteoarthritis

Effectiveness of the intra-articular injection of platelet rich plasma in the treatment of patients with primary knee osteoarthritis

Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study

A Review of Commercially Available Point-of-Care Devices to Concentrate Bone Marrow for the Treatment of Osteoarthritis and Focal Cartilage Lesions

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