Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

Mounier, Rémi; Pialoux, Vincent; Roels, Belle; Thomas, Claire; Millet, Grégoire P.; Mercier, Jacques; Coudert, J; Fellmann, Nicole; Clottes, Eric

doi:10.1007/s00421-008-0928-y

Cited by 29 publications

(23 citation statements)

References 44 publications

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“…It can be speculated that such muscle-derived VEGF leaks into the serum thereafter and might be the reason for the observed increases, even though the performed exercise and kinetic of VEGF levels were different. Furthermore, blood cells (leukocytes) were also suggested as a source for VEGF expression (Mounier et al 2009). …”

Section: Discussionmentioning

confidence: 98%

Effects of acid–base balance and high or low intensity exercise on VEGF and bFGF

et al. 2010

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The purpose of the present study was to compare the acute hormonal response to a short-term high-intensity training (HIT) versus a high-volume endurance training (HVT) and to investigate the effects of acid-base status on cytokines involved in angiogenesis (VEGF and bFGF). Eleven subjects participated in three experimental trials. Two times subjects performed four 30-s "all-out" exercise bouts on a cycle ergometer separated by 5-min rest each, at which subjects either received bicarbonate (HIT (B)) or a placebo (HIT (P)) before the exercise. The third exercise trail consisted of a constant load exercise for 1 h at 50% peak power output (PPO). Venous blood samples were taken under resting conditions, 10, 60, and 240 min after each exercise condition to determine VEGF and bFGF serum concentrations. Capillary blood samples were taken to determine lactate concentrations and blood gas parameters. Mean pH values were significantly higher during HIT (B) compared to HIT (P). Serum VEGF concentration was significantly increased 10-min post-exercise in both HIT interventions. HVT showed no significant effects on VEGF levels. The diminished acidosis during HIT (B) had no effects on the VEGF response. There were no significant changes in bFGF in response to HIT or HVT. The present study suggests that HIT is a stimulus for exercise-induced VEGF secretion. These findings might be relevant for the arrangement of training, due to the fact that most of the training is often performed at low intensities possibly leading to an insufficient stimulus for VEGF secretion and angiogenesis.

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Section: Discussionmentioning

confidence: 98%

Effects of acid–base balance and high or low intensity exercise on VEGF and bFGF

et al. 2010

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“…Although the scientific evidence directly linking IHT and improved anaerobic or sprint performance remains unsubstantiated (20), IHE coupled with a training intensity sufficient to induce changes in the partial pressure of inspired oxygen (P I O 2 ) may lead to favorable, skeletal muscle-related biochemical and genetic adaptations (10,22,24). Combined, IHT may elicit increases in tissue perfusion capacity, enhance rates of phosphocreatine (PCr) resynthesis, and upregulate relevant gene expression (e.g., hypoxia-inducible factor-1 [HIF-1a], carbonic anhydrase III, monocarboxylate transporter-4 [MCT-4]) (16,23).…”

Section: Introductionmentioning

confidence: 99%

Differential Effect of Metabolic Alkalosis and Hypoxia on High-Intensity Cycling Performance

Flinn

Herbert²,

Graham³

et al. 2014

Journal of Strength and Conditioning Research

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Flinn, S, Herbert, K, Graham, K, and Siegler, JC. Differential effect of metabolic alkalosis and hypoxia on high-intensity cycling performance. J Strength Cond Res 28(10): 2852-2858, 2014-The purpose of this study was to investigate the effects of sodium bicarbonate (NaHCO 3 ) ingestion and acute hypoxic exposure on repeated bouts of high-intensity cycling to task failure. Twelve subjects completed 4 separate intermittent cycling bouts cycling bouts to task failure (120% peak power output for 30-second interspersed with 30-second active recovery) under the following conditions: normoxia (F I O 2 % at 20.93%) alkalosis (NA), normoxia placebo (NP), hypoxia (F I O 2 % at 14.7%) alkalosis (HA), and hypoxia placebo (HP). For the NA and HA trials, the buffer solution (0.3 g$kg 21 of NaHCO 3 ) was dispensed into gelatin capsules and consumed over 90 minutes with 1 L of water. Whole-blood acidbase findings demonstrated metabolic alkalosis in both NA and HA before exercise (HCO 3 2 : 32.8 6 1.8 mmol$L 21 ). Time to task failure was significantly impaired in the hypoxic conditions (NA: 199.1 6 62.3 seconds, NP: 183.8 6 45.0 seconds, HA: 127.8 6 27.9 seconds, HP: 133.3 6 28.7 seconds; p , 0.001; h 2 = 0.7). There was no difference between the HA and HP conditions (p = 0.41); however the 2 normoxic conditions approached significance with the NA condition on average resulting in approximately 15-second improvement in time to task failure (p = 0.09). These findings suggest that an acute decline in F I O 2 % consistent with hypoxic exposure is more inhibiting than metabolic acidosis during intermittent highintensity cycling to task failure. In application, the use of hypoxia and NaHCO 3 concurrently to improve performance under these conditions does not seem warranted. Figure 2. Time to task failure (s) for the 4 trial conditions (NA, NP, HA, and HP). Data are presented as mean 6 SD. *Significantly different from hypoxic conditions (p , 0.001). NA = normoxia alkalosis; NP = normoxia placebo; HA = hypoxia alkalosis; HP = hypoxia placebo.Figure 3. Individual performance data represented as responders, nonresponders, and negative responders. Journal of Strength and Conditioning Research the TM | www.nsca.com

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“…In general, the effects of endurance training on the activity of the HIF pathway in human skeletal muscle under hypoxic conditions appear to be definitely higher than those under normoxic conditions (219,422,446), although there are several exceptions (97,241), indicating that combining hypoxia with exercise training appears to improve some aspects of muscle O 2 transport and/or metabolism (Fig. 14) (219).…”

Section: Exercise and Redox Signalingmentioning

confidence: 99%

Oxygen Consumption and Usage During Physical Exercise: The Balance Between Oxidative Stress and ROS-Dependent Adaptive Signaling

Radák

Zhao

Koltai

et al. 2013

Antioxidants & Redox Signaling

503

425

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The complexity of human DNA has been affected by aerobic metabolism, including endurance exercise and oxygen toxicity. Aerobic endurance exercise could play an important role in the evolution of Homo sapiens, and oxygen was not important just for survival, but it was crucial to redox-mediated adaptation. The metabolic challenge during physical exercise results in an elevated generation of reactive oxygen species (ROS) that are important modulators of muscle contraction, antioxidant protection, and oxidative damage repair, which at moderate levels generate physiological responses. Several factors of mitochondrial biogenesis, such as peroxisome proliferator-activated receptor-c coactivator 1a (PGC-1a), mitogen-activated protein kinase, and SIRT1, are modulated by exercise-associated changes in the redox milieu. PGC-1a activation could result in decreased oxidative challenge, either by upregulation of antioxidant enzymes and/or by an increased number of mitochondria that allows lower levels of respiratory activity for the same degree of ATP generation. Endogenous thiol antioxidants glutathione and thioredoxin are modulated with high oxygen consumption and ROS generation during physical exercise, controlling cellular function through redox-sensitive signaling and proteinprotein interactions. Endurance exercise-related angiogenesis, up to a significant degree, is regulated by ROSmediated activation of hypoxia-inducible factor 1a. Moreover, the exercise-associated ROS production could be important to DNA methylation and post-translation modifications of histone residues, which create heritable adaptive conditions based on epigenetic features of chromosomes. Accumulating data indicate that exercise with moderate intensity has systemic and complex health-promoting effects, which undoubtedly involve regulation of redox homeostasis and signaling. Antioxid. Redox Signal. 18, 1208Signal. 18, -1246

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Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

Cited by 29 publications

References 44 publications

Effects of acid–base balance and high or low intensity exercise on VEGF and bFGF

Effects of acid–base balance and high or low intensity exercise on VEGF and bFGF

Differential Effect of Metabolic Alkalosis and Hypoxia on High-Intensity Cycling Performance

Oxygen Consumption and Usage During Physical Exercise: The Balance Between Oxidative Stress and ROS-Dependent Adaptive Signaling

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