Effect of Interleukin-7 on In Vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Developmental Potential after Parthenogenetic Activation

Oh, Dongjin; Lee, Joohyeong; Kim, Eunhye; Hwang, Seon‐Ung; Yoon, Jong Hwan; Cai, Lei; Kim, Mirae; Kim, Gahye; Choi, Hyerin; Hyun, Sang Hwan

doi:10.3390/ani11030741

Cited by 7 publications

(8 citation statements)

References 65 publications

(24 reference statements)

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“…IL-7 treatment regulates oxidative stress (21). Consistent with the results of the present study, we observed that IL-7 supplementation during porcine IVC increased intracellular GSH levels and reduced ROS levels in 4-cell embryos, provoking ZGA.…”

Section: Discussionsupporting

confidence: 92%

“…IL-7 was also identified as a potential candidate for oocyte quality screening tests ( 22 ). Recently as well, it was demonstrated that IL-7 improves oocyte maturation and developmental potential via the regulation of apoptosis-related genes when administered within an IVM system for sheep ( 40 ) and pigs ( 21 ). Thus, we speculated that IL-7 possibly plays a similar role in porcine IVC, and in this regard, our results demonstrated that IL-7 treatment favored the in vitro development of porcine preimplantation embryos after PA, and reduced fragmentation in day 2 embryos.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been observed that a moderate concentration of IL-7 during IVM increases ROS levels in ovine oocytes ( 40 ). In one of our previous studies, we performed an experiment using a porcine IVM system to determine whether IL-7 treatment regulates oxidative stress ( 21 ). Consistent with the results of the present study, we observed that IL-7 supplementation during porcine IVC increased intracellular GSH levels and reduced ROS levels in 4-cell embryos, provoking ZGA.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it modulates B-cell lymphoma 2 (BCL2) family members ( 18 ). In the reproductive system, IL-7 has been detected in both human ( 19 , 20 ) and porcine follicular fluid (FF) ( 21 ), and its functional roles in oocytes and surrounding somatic cells in various species have been reported ( 22 ). Additionally, it has been observed that IL-7 treatment modulates the phosphorylation of AKT and STAT5 proteins and inhibits apoptosis in rat granulosa cells ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

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Interleukin-7 enhances in vitro development and blastocyst quality in porcine parthenogenetic embryos

Choi

Kim

et al. 2022

Front. Vet. Sci.

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Interleukin-7 (IL-7), a vital factor that affects cell development, proliferation, and survival, plays an important role in oocyte maturation. However, its role in embryonic development remains unknown. Therefore, in this study, we aimed to investigate the effects of IL-7 supplementation on in vitro culture (IVC) of porcine embryos after parthenogenetic activation (PA) based on characteristics such as cleavage, blastocyst formation rate, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in cleaved embryos, total cell number, apoptosis rate, and cell lineage specification in blastocysts. Immunofluorescence revealed that IL-7 and its receptor, IL-7Rα (IL-7R) localized in the cytoplasm of porcine parthenote embryos. By supplementing the IVC medium (PZM5) with various concentrations of IL-7, an optimal concentration that enhanced embryonic development, promoted intracellular GSH, and decreased ROS levels in the cleavage stage during porcine embryo IVC was determined. Investigation of mRNA expression patterns via qRT-PCR suggested that IL-7 possibly regulated maternal mRNA clearance and zygotic genome activation. Furthermore, IL-7 supplementation reduced blastocyst apoptosis, enhanced the expression of the inner cell mass marker SOX2, and phosphorylated STAT5 levels in the blastocysts. Moreover, it altered the transcription patterns of genes that regulate apoptosis, IL-7 signaling, and development. Thus, we demonstrated the localization of IL-7 and IL-7R in porcine preimplantation embryos in vitro for the first time. Furthermore, we suggest that IL-7 supplementation can be employed to enhance embryonic development and blastocyst quality based on the activation of the transcripts of genes that are involved in developmental competence and IL-7 signaling during in vitro porcine embryo development following PA.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interleukin-7 enhances in vitro development and blastocyst quality in porcine parthenogenetic embryos

Choi

Kim

et al. 2022

Front. Vet. Sci.

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“…Similar to many other studies [33][34][35], this study showed that ROS was upregulated, and oxidative stress was induced in porcine oocytes as a result of rotenone treatment. In the previous study [36][37][38], GSH detected using Cell Tracker was the opposite concept of ROS, and the rotenone treated group decreased compared to the control group. As oxidative stress was speculated to be the result of rotenonemediated suppression of mitochondrial function, mitochondrial function was also investigated.…”

Section: Discussionmentioning

confidence: 84%

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Sun

Jiang

et al. 2022

PLoS ONE

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Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex Ⅰ. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information on the effects of rotenone exposure in porcine oocytes during in vitro maturation, and the mechanisms underlying these effects have not been determined. The Cumulus-oocyte complexes were supplemented with different concentrations of rotenone to elucidate the effects of rotenone exposure on the meiotic maturation of porcine oocytes during in vitro maturation for about 48 hours. First, we found that the maturation rate and expansion of cumulus cells were significantly reduced in the 3 and 5 μM rotenone-treated groups. Subsequently, the concentration of rotenone was determined to be 3 μM. Also, immunofluorescence, western blotting, and image quantification analyses were performed to test the rotenone exposure on the meiotic maturation, total and mitochondrial ROS, mitochondrial function and biogenesis, mitophagy and apoptosis in porcine oocytes. Further experiments showed that rotenone treatment induced mitochondrial dysfunction and failure of mitochondrial biogenesis by repressing the level of SIRT1 during in vitro maturation of porcine oocytes. In addition, rotenone treatment reduced the ratio of active mitochondria to total mitochondria, increased ROS production, and decreased ATP production. The levels of LC3 and active-caspase 3 were significantly increased by rotenone treatment, indicating that mitochondrial dysfunction induced by rotenone increased mitophagy but eventually led to apoptosis. Collectively, these results suggest that rotenone interferes with porcine oocyte maturation by inhibiting mitochondrial function.

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Effects of indented zona pellucida on oocyte growth and development explored from changes of gene expression in cumulus cells

Jiang

Guang

et al. 2023

Arch Gynecol Obstet

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Effect of Interleukin-7 on In Vitro Maturation of Porcine Cumulus-Oocyte Complexes and Subsequent Developmental Potential after Parthenogenetic Activation

Cited by 7 publications

References 65 publications

Interleukin-7 enhances in vitro development and blastocyst quality in porcine parthenogenetic embryos

Interleukin-7 enhances in vitro development and blastocyst quality in porcine parthenogenetic embryos

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Effects of indented zona pellucida on oocyte growth and development explored from changes of gene expression in cumulus cells

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