Effect of interferon on synthesis of ssRNA in reovirus type 3-infected L cell cultures

Gauntt, Charles J.

doi:10.1016/0006-291x(72)90966-7

Cited by 15 publications

(3 citation statements)

References 14 publications

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“…The total amount of[3H]methyl residues in RNA after the specified steps of the experiment were as listed below. From step 1 incubations: (1) S30c + [3H]SAM, 6,944; (2) S30c + reo mRNAu + [3H]SAM, 31,920; (3) S30INT + [3H]SAM, 5,488; (4) S30INT + reo mRNAu + [3H]SAM, 19,040; from step 3 incubations: (5) S30c + total RNA from (1), 6,685; (6) S30c + total RNA from (2), 27,895; (7) S30c + total RNA from (3), 4,095; (8) S30c + total RNA from (4),17,167; (9) S30c + total RNA from (1) + [3H]SAM, 40,565; (10) S30c + total RNA from (2) + [3H]SAM, 70,420; (11) S30c + total RNA from (3) + [3H]SAM, 33,635; (12) (5) to (8), served to determine the amount of label from step 1 still present after steps 2 and 3. It was established that total labeled RNA was as stable during step 3 incubations in S301NT as in S30C.…”

Section: Impairment Of Reo Mrnau Methylation Inmentioning

confidence: 99%

Impairment of reovirus mRNA methylation in extracts of interferon-treated Ehrilich ascites tumor cells: further characteristics of the phenomenon

Sen¹,

Shaila²,

Lebleu³

et al. 1977

J Virol

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We reported earlier that the methylation of unmethylated reovirus mRNA (reo mRNAu) by the cellular methylating enzymes is impaired in extracts of uninfected, interferon-treated Ehrlich ascites tumor cells (S30INT). We find now that after the methylation of reo mRNAu has stopped in S301NT, the RNA can be reisolated and further methylated in an extract of control cells (S30C). Thus the impairment of methylation in S301NT cannot be due to cleavage or irreversible inactivation of reo mRNAu. Freshly added reo mRNAu can be methylated in S301NT in which the methylation of previously added reo mRNAu has stopped. This indicates that the impairment is not due to the depletion ofS-adenosylmethionine (the methyl donor), the accumulation of S-adenosylhomocysteine (an inhibitor of methylation), or the irreversible inactivation of the methylating enzymes. It may be due, however, to the unavailability of reo mRNAu for methylation. The extent of the impairment of reo mRNAu methylation in S301NT

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Section: Impairment Of Reo Mrnau Methylation Inmentioning

confidence: 99%

Impairment of reovirus mRNA methylation in extracts of interferon-treated Ehrilich ascites tumor cells: further characteristics of the phenomenon

Sen¹,

Shaila²,

Lebleu³

et al. 1977

J Virol

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“…The target viral genome function inhibited was shown for two RNA viruses to be primarily the viral genome transcription (vesicular stomatitis virus [ 1, 21, reovirus [ 31).…”

Section: Introductionmentioning

confidence: 99%

Inhibition by interferon of SV40 tumor antigen formation in cells injected with SV40 cRNA transcribed in vitro

Graessmann

Hoffmann

et al. 1974

FEBS Letters

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“…Inhibition of viral RNA synthesis is taken as a measure of interferon titer. This method offers the advantage of being potentially applicable to both cytolytic and poorly or noncytolytic viruses; its drawbacks are that it generally requires high multiplicities of infection, large culture flasks, and mechanical removal of cells before isotope counting (1,6,10,11,13,22). Growing the cells directly in scintillation vials reduces handling to some extent (19).…”

mentioning

confidence: 99%

Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester

Richmond¹,

Polatnick²,

Knudsen³

1980

Appl Environ Microbiol

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A microassay for interferon is described which uses target cells grown in microculture wells, [3H]uridine to measure vesicular stomatitis virus replication in target cells, and a multiple automated sample harvester to collect the radioactively labeled viral ribonucleic acid onto glass fiber filter disks. The disks were placed in minivials, and radioactivity was counted in a liquid scintillation spectrophotometer. Interferon activity was calculated as the reciprocal of the highest titer which inhibited the incorporation of [3H]uridine into viral ribonucleic acid by 50%. Interferon titers determined by the microassay were similar to the plaque reduction assay when 100 plaque-forming units of challenge vesicular stomatitis virus was used. However, it was found that the interferon titers decreased approximately 2-fold for each 10-fold increase in the concentration of challenge vesicular stomatitis virus when tested in the range of 102 to 105 plaque-forming units. Interferon titers determined by the microassay show a high degree of repeatability, and the assay can be used to measure small and large numbers of interferon samples.

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Effect of interferon on synthesis of ssRNA in reovirus type 3-infected L cell cultures

Cited by 15 publications

References 14 publications

Impairment of reovirus mRNA methylation in extracts of interferon-treated Ehrilich ascites tumor cells: further characteristics of the phenomenon

Impairment of reovirus mRNA methylation in extracts of interferon-treated Ehrilich ascites tumor cells: further characteristics of the phenomenon

Inhibition by interferon of SV40 tumor antigen formation in cells injected with SV40 cRNA transcribed in vitro

Microassay for interferon, using [3H]uridine, microculture plates, and a multiple automated sample harvester

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