Effect of interfacial serum proteins on the cell membrane disruption induced by amorphous silica nanoparticles in erythrocytes, lymphocytes, malignant melanocytes, and macrophages

Shinto, Hiroyuki; Fukasawa, Tomonori; Yoshisue, Kosuke; Tsukamoto, Nanami; Aso, Saki; Hirohashi, Yumiko; Seto, Hirokazu

doi:10.1016/j.colsurfb.2019.05.067

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…[ 57 ] found that cell membrane disruption induced by SiO 2 NPs in different types of cells, including erythrocytes, lymphocytes, malignant melanocytes, and macrophages. In subsequent study, they found that interfacial serum proteins reduced the membranolysis [ 58 ]. An in vitro study using bacteria demonstrated that GONWs exhibited antibacterial activity due to their sharp edges that can interact with cell membranes [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

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Background Silica nanoparticles (SiO2 NPs) are extensively applied in the biomedical field. The increasing medical application of SiO2 NPs has raised concerns about their safety. However, studies on SiO2 NP-induced retinal toxicity are lacking. Methods We investigated the retinal toxicity of SiO2 NPs with different sizes (15 and 50 nm) in vitro and in vivo along with the underlying mechanisms. The cytotoxicity of SiO2 NPs with different sizes was assessed in R28 human retinal precursor cells by determining the ATP content and LDH release. The cell morphologies and nanoparticle distributions in the cells were analyzed by phase-contrast microscopy and transmission electron microscopy, respectively. The mitochondrial membrane potential was examined by confocal laser scanning microscopy. The retinal toxicity induced by SiO2 NPs in vivo was examined by immunohistochemical analysis. To further investigate the mechanism of retinal toxicity induced by SiO2 NPs, reactive oxygen species (ROS) generation, glial cell activation and inflammation were monitored. Results The 15-nm SiO2 NPs were found to have higher cytotoxicity than the larger NPs. Notably, the 15-nm SiO2 NPs induced retinal toxicity in vivo, as demonstrated by increased cell death in the retina, TUNEL-stained retinal cells, retinal ganglion cell degeneration, glial cell activation, and inflammation. In addition, The SiO2 NPs caused oxidative stress, as demonstrated by the increase in the ROS indicator H2DCF-DA. Furthermore, the pretreatment of R28 cells with N-acetylcysteine, an ROS scavenger, attenuated the ROS production and cytotoxicity induced by SiO2 NPs. Conclusions These results provide evidence that SiO2 NPs induce size-dependent retinal toxicity and suggest that glial cell activation and ROS generation contribute to this toxicity. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

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“…The biosafety of MSNs is related to their surface modification. Studies have shown that the cytotoxicity of MSNs is mainly related to their surface silanol groups, which may nonspecifically bind to certain proteins on the cell membrane and cause cell lysis and necrosis (Slowing et al, 2009;Lin and Haynes, 2010;Shinto et al, 2019;Tsamesidis et al, 2020). The surface properties of MSNs have an impact on their biodistribution and biocompatibility.…”

Section: Influence Of Msn Shape Size and Surface Modificationmentioning

confidence: 99%

Applications and Biocompatibility of Mesoporous Silica Nanocarriers in the Field of Medicine

et al. 2022

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Mesoporous silica nanocarrier (MSN) preparations have a wide range of medical applications. Studying the biocompatibility of MSN is an important part of clinical transformation. Scientists have developed different types of mesoporous silica nanocarriers (MSNs) for different applications to realize the great potential of MSNs in the field of biomedicine, especially in tumor treatment. MSNs have achieved good results in diagnostic bioimaging, tissue engineering, cancer treatment, vaccine development, biomaterial application and diagnostics. MSNs can improve the therapeutic efficiency of drugs, introduce new drug delivery strategies, and provide advantages that traditional drugs lack. It is necessary not only to innovate MSNs but also to comprehensively understand their biological distribution. In this review, we summarize the various medical uses of MSN preparations and explore the factors that affect their distribution and biocompatibility in the body based on metabolism. Designing more reasonable therapeutic nanomedicine is an important task for the further development of the potential clinical applications of MSNs.

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“…The PMEA used in this study is known to be a biocompatible polymer. ,, Particles composed of amorphous silica are also considered to have low toxicity to living organisms for particles of this size. − Therefore, elastomers composited with these materials are also considered safe for living organisms and the environment. These results indicate that elastomers that exhibit fade-resistant structural coloration formed from safe materials can be materials that combine shape-independent stable coloration with mechanical strength.…”

Section: Resultsmentioning

confidence: 99%

“…PMEA obtained from polymerization of MEA is well known as a biocompatible polymer. , Monodisperse SiO 2 particles with a particle size of 180 nm (hereafter referred to as SiO 2 particles, Figure S1) were purchased from Fuji Chemical. Although there are various toxicity concerns for particles composed of amorphous silica smaller than 100 nm, previous studies have reported that amorphous silica particles of the size used in this study have relatively low toxicity. − …”

Section: Methodsmentioning

confidence: 99%

Optically Transparent and Color-Stable Elastomer with Structural Coloration under Elongation

Miyake,

He,

Asai

et al. 2023

Langmuir

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Effect of interfacial serum proteins on the cell membrane disruption induced by amorphous silica nanoparticles in erythrocytes, lymphocytes, malignant melanocytes, and macrophages

Cited by 11 publications

References 39 publications

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Applications and Biocompatibility of Mesoporous Silica Nanocarriers in the Field of Medicine

Optically Transparent and Color-Stable Elastomer with Structural Coloration under Elongation

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