Effect of interaction between occupational stress and polymorphisms of MTHFR gene and SELE gene on hypertension

Abstract

Background Gene-environment interaction is related to the prevalence of hypertension, but the impact of genetic polymorphisms on hypertension may vary due to different geography and population. Objective To explore the impact of the interaction among occupational stress and MTHFR gene and SELE gene polymorphism on the prevalence of hypertension in Xinjiang oil workers. Methods A case-control study was conducted on 310 oil workers. In an oilfield base in Karamay City, Xinjiang, 155 hypertensive patients aged 18~60 years old with more than one year of service were selected as the case group, and 155 oil workers without hypertension were selected as the control group according to the 1:1 matching principle (matching conditions: the gender and shift were the same. The age is around 2 years old). The Occupational Stress Scale was used to evaluate the degree of occupational stress, PCR technique was used to detect MTHFR and SELE gene polymorphism, Logistic regression analysis was used to analyze the effects of gene and occupational stress on hypertension, and gene-gene and gene-environment interactions were analyzed by generalized multi-factor dimension reduction method. Results The G98T polymorphism of SELE gene (χ2 = 6.776, P = 0.034), the C677T (χ2 = 7.130, P = 0.028) and A1298C (χ2 = 12.036, P = 0.002) loci of MTHFR gene and the degree of occupational stress (χ2 = 11.921, P = 0.003) were significantly different between the case group and the control group. The genotypes GT at the G98T polymorphism of the SELE gene (OR = 2.151, 95% CI [1.227–3.375]), and the dominant model (AC/CC vs AA, OR = 1.925, 95% CI [1.613–3.816]); AC and CC at the A1298C polymorphism of the MTHFR gene (ORAC = 1.917, 95% CI [1.064–3.453]; ORCC = 2.233, 95% CI [1.082–4.609]), the additive model (CC vs AA, OR = 2.497, 95% CI [1.277–4.883]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200–3.373]); at the C677T polymorphism of the MTHFR gene CT and TT (ORCT = 1.913, 95% CI [1.085–3.375]; ORTT = 3.117, 95% CI [1.430–6.795]), the additive model (CC vs AA, OR = 1.913, 95% CI [1.085–3.375]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200–3.373]), which could increase hypertension risk (P < 0.05). The gene-gene interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene, and the gene-occupational stress interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene and the occupational stress. Conclusion The interaction of gene mutation and occupational stress in Xinjiang oil workers maybe increase the risk of hypertension.

Bioinformatics Analyzes of Selectins Polymorphisms Reveal New Potential Cancer Biomarkers

“…Several approaches have been developed to target E-selectin, including monoclonal antibodies and small molecule inhibitors and have shown promise in preclinical studies by reducing tumor growth and metastasis [75][76][77][78][79]. SELE polymorphisms have been reported as possible risk indicators for a number of medical conditions such as hypertension in cases of occupational stress [80], risk of coronary artery disease [81], enlargement of renal cyst in patients with polycystic kidney disease [82], type 2 diabetes [83] and subclinical atherosclerosis and increased platelet activity in systemic lupus erythematosus [84]. We demonstrated that rs1534904 (T/A and T/G) could provoke important alterations in DNA.…”

Associations of methylene tetrahydrofolate reductase (MTHFR) polymorphism with hepatocellular carcinoma in Egyptian population

“…MTHFR enzyme is coded by the MTHFR gene that is located on the short arm of chromosome 1 (1p36.3) and there are two isoforms in humans [13]. The first isoform is about 77 kDa protein, and the second is 70 kDa [14].…”

A study on the correlation between MTHFR and folic acid combined with trace elements for the prevention of fetal malformations in the first trimester of pregnancy

“…Since the 20th century, experimental and clinical studies have confirmed that fetal malformations are closely related to 5,10-methylenetetrahydrofolate reductase (MTHFR), folic acid (FA), and trace elements, and will affect the fetal condition of early pregnant women. [14][15][16][17][18] Genetic susceptibility genes such as the MTHFR gene may be involved in the occurrence and development of fetal malformations. [17] Based on this foundation, this article takes women in early pregnancy as the starting point to study the value and correlation of MTHFR, FA, and trace elements in preventing fetal malformations, in order to provide reference for fetal assessment during pregnancy.…”