Effect of intensive exercise‐induced testicular gametogenic and steroidogenic disorders in mature male Wistar strain rats: a correlative approach to oxidative stress

Manna, Indranil; Jana, Kuladip; Samanta, Prabhat Kumar

doi:10.1046/j.1365-201x.2003.01095.x

Cited by 64 publications

(92 citation statements)

References 50 publications

(76 reference statements)

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“…91 However, excess exercise can have the opposite effect, causing oxidative stress in the testes and generating high levels of lipid peroxidation in association with significant declines in the activities of key antioxidant enzymes including SOD, catalase, GST and GPx. 92 Such stress has a significant inhibitory effect on the both steroidogenesis and germ cell differentiation within the testes. The fact that these effects can be reversed by the administration of an antioxidant, α-tocopherol succinate, confirms the importance of oxidative stress in the aetiology of such exercise-dependent testicular dysfunction.…”

Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%

Antioxidant Systems and Oxidative Stress in the Testes

Aitken

Roman

2009

Advances in Experimental Medicine and Biology

156

105

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Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%

Antioxidant Systems and Oxidative Stress in the Testes

Aitken

Roman

2009

Advances in Experimental Medicine and Biology

156

105

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“…Low SOD activity in the testis induces poor spermatogenic function [18] because of an increase in ROS concentration [9]. The T secretion by the testes and testicular SOD activity in all of the AZ-dogs in the present study increased after the GnRH-A injections.…”

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confidence: 48%

“…69(5): 561-562, 2007 Production of reactive oxygen species (ROS) by testicular tissue [13,18] and sperm [1,17] has been reported in male animals. Oxidative stress caused by elevated ROS concentrations induces spermatogenic dysfunction [9] and poor semen quality [10], and high ROS concentrations in the seminal plasma of dogs also causes low sperm motility [15]. Superoxide dismutase (SOD) is known to be the most important antioxidant enzyme in seminal plasma [5], and SOD activity has been detected in canine seminal plasma [2].…”

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confidence: 99%

Changes in the Plasma Testosterone Level and Testicular Superoxide Dismutase Activity of 5 Azoospermic Beagles after GnRH Analogue Injections

Kawakami

Kuroda

Hori

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The peripheral blood plasma testosterone (T) levels and superoxide dismutase (SOD) activity were measured in 5 azoospermic (AZ-) beagles. The mean values in the AZ-dogs were significantly lower than in 7 control beagles (P<0.001). Subcutaneous injections of 1 µg/kg GnRH analogue three times weekly in the AZ-dogs induced significant increases in mean T level and SOD activity (P<0.05) and improvement in spermatogenesis. Thus, spermatogenic function in the dog appears to be maintained by T and normal SOD activity in the testis. KEY WORDS: azoospermia, canine, SOD.J. Vet. Med. Sci. 69(5): 561-562, 2007 Production of reactive oxygen species (ROS) by testicular tissue [13,18] and sperm [1,17] has been reported in male animals. Oxidative stress caused by elevated ROS concentrations induces spermatogenic dysfunction [9] and poor semen quality [10], and high ROS concentrations in the seminal plasma of dogs also causes low sperm motility [15]. Superoxide dismutase (SOD) is known to be the most important antioxidant enzyme in seminal plasma [5], and SOD activity has been detected in canine seminal plasma [2]. Leydig [8] and Sertoli cells [11] have been reported to produce SOD in the testis. Low SOD activity in seminal plasma causes infertility in humans [14]. Although the cause of spermatogenic arrest in the dog is not well understood [4], the authors have reported that GnRH analogue (-A) injection transiently improved the semen quality of some dogs with poor testosterone (T) secretory function in the testes and spermatogenic dysfunction [7]. In the present study the plasma T levels and testicular SOD activities of azoospermic (AZ-) dogs were measured after GnRH-A injections in order to investigate the cause of spermatogenic dysfunction and the interaction between T secretory function and SOD activity in the testes.Five AZ-beagles (4-7 years old) cared for at our university were used in this study. The 5 dogs had previously been diagnosed with azoospermia based on examinations of ejaculated semen collected by digital manipulation at weekly intervals for 4 weeks. Seven beagles (3-6 years old) with normal semen quality (the total number of sperm was more than 300 × 10 6 , more than 80% of sperm were actively motile, and less than 10% of sperm were abnormal) were used as controls.The GnRH-A used in this study was GnRH ethylamide (D-Ser-(tBu)-des-gly-NH 2 ; Buserelin, Hoechst Inc., Germany). The AZ-dogs were given 3 weekly subcutaneous injections of 1 µg GnRH-A per kilogram body weight according to the method described previously [7]. Blood samples were collected from a peripheral vein 5 weeks before and 10 weeks after the first injection of GnRH-A. Since the plasma T level of male dogs fluctuates diurnally [6,16], blood samples were collected 3 times a day (09:00, 13:00, 17:00). Plasma T levels were determined by radioimmunoassay using the method described by DePalatis et al. [3], and the mean T level was calculated for the 3 plasma samples collected each day.Tissue was collected twice from the right testis of ...

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“…Reduction in the basal and total testosterone level was observed after intensive endurance exercise in the humans, which could possibly lead to the disruption of testosterone dependent processes (Manna et al 2003;Hackney 2008;Jana et al 2008). Also, intensive swimming exercise has been linked to a vast range of impairments regarding the male fertility, such as reduced spermatic production (Mingoti et al 2003), reduced somatic index of the testes, epididymis, prostate and seminal vesicle (Manna et al 2003), reduced number of preleptotine spermatocytes, midpachytene spermatocytes and stage 7 spermatids (Manna et al 2003;Jana et al 2008), and reduced epididymal sperm count (Jana et al 2008). As observed for the increased colon cancer susceptibility, the damages caused to male fertility by the exercise appear to be associated with an increase in oxidative stress (Manna et al 2003;Manna et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Also, intensive swimming exercise has been linked to a vast range of impairments regarding the male fertility, such as reduced spermatic production (Mingoti et al 2003), reduced somatic index of the testes, epididymis, prostate and seminal vesicle (Manna et al 2003), reduced number of preleptotine spermatocytes, midpachytene spermatocytes and stage 7 spermatids (Manna et al 2003;Jana et al 2008), and reduced epididymal sperm count (Jana et al 2008). As observed for the increased colon cancer susceptibility, the damages caused to male fertility by the exercise appear to be associated with an increase in oxidative stress (Manna et al 2003;Manna et al 2004). Animal models are indispensible for cancer research, and chemical induction is the most common method used for developing the colon cancer (Hoffman-Goetz 2003).…”

Section: Introductionmentioning

confidence: 99%