Effect of integrin α7 on cell proliferation, invasion, apoptosis and the PI3K/AKT pathway, and its association with clinicopathological features in endometrial cancer

Mao, Liang; Liu, Cong; Tao, Lei; Guo, Shichao; Min, Jie

doi:10.3892/ol.2022.13612

Cited by 5 publications

(4 citation statements)

References 28 publications

(42 reference statements)

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“…For instance, Ming et al detected ITGA7‐expressing cells in most esophageal squamous cell carcinoma tissues but not in corresponding nontumor tissues 31 . Lv et al and Liang et al found that the ITGA7 was mainly expressed in tumor tissues compared with paired adjacent tissues 40,41 . However, in papillary thyroid carcinoma, ITGA7 was mainly expressed in nontumor tissues compared to tumor tissues 42 .…”

Section: Discussionmentioning

confidence: 99%

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ITGA7 loss drives the differentiation of adipose‐derived mesenchymal stem cells to cancer‐associated fibroblasts

Liu,

Gao,

et al. 2024

Molecular Carcinogenesis

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Cancer‐associated fibroblasts (CAFs) represent a major cellular component of the tumor (pre‐)metastatic niche and play an essential role in omental dissemination of ovarian cancer. The omentum is rich in adipose, and adipose‐derived mesenchymal stem cells (ADSCs) have been identified as a source of CAFs. However, the molecular events driving the phenotype shift of ADSCs remain largely unexplored. In this research, we focus on integrins, transmembrane receptors that have been widely involved in cellular plasticity. We found that integrin α7 (ITGA7) was the only member of the integrin family that positively correlated with both overall survival and progression‐free survival in ovarian cancer through GEPIA2. The immunohistochemistry signal of ITGA7 was apparent in the tumor stroma, and a lower omental ITGA7 level was associated with metastasis. Primary ADSCs were isolated from the omentum of patients with ovarian cancer and identified by cellular morphology, biomarkers, and multilineage differentiation. The conditional medium of ovarian cancer cells induced ITGA7 expression decrease and phenotypic changes in ADSCs. Downregulation of ITGA7 in primary omental ADSCs led to decrease in stemness properties and emerge of characteristic morphology and biomarkers of CAFs. Moreover, the conditioned medium of ADSCs with ITGA7 depletion exhibited enhanced abilities to improve the migration and invasion of ovarian cancer cells in vitro. Overall, these findings indicate that loss of ITGA7 may induce the differentiation of ADSCs to CAFs that contribute to a tumor‐supportive niche.

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Section: Discussionmentioning

confidence: 99%

“…IHC staining results showed that ITGA7 was expressed predomi- was mainly expressed in tumor tissues compared with paired adjacent tissues. 40,41 However, in papillary thyroid carcinoma, ITGA7…”

Section: Discussionmentioning

confidence: 99%

ITGA7 loss drives the differentiation of adipose‐derived mesenchymal stem cells to cancer‐associated fibroblasts

Liu,

Gao,

et al. 2024

Molecular Carcinogenesis

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“…A previous study reported that the L1CAM [386], HSD17B2 [387], GRP (gastrin releasing peptide) [388], FABP4 [389], SOX6 [390]. MMP12 [391], APOD (apolipoprotein D) [392], LAG3 [393], CST1 [394], FLT1 [395], DHCR24 [396], PRL (prolactin) [397], WNT5A [398], TIMP3 [399], CD24 [400], LHCGR (luteinizing hormone/choriogonadotropin receptor) [401], MMRN1 [402], CD4 [403], ADAMTS5 [404], ADAMTS1 [405], PADI2 [406], MARK1 [407], KL (klotho) [408], PLAU (plasminogen activator, urokinase) [409], SOX8 [410], CRABP2 [411], PTPRD (protein tyrosine phosphatase receptor type D) [412], EPCAM (epithelial cell adhesion molecule) [413], IRX2 [414], SEMA3B [415], CYP1A1 [416], PDGFD (platelet derived growth factor D) [417], LEPR (leptin receptor) [418], APOE (apolipoprotein E) [419], CASP1 [420], MGLL (monoglyceride lipase) [421], NID1 [422], ABCG2 [423], ACE (angiotensin I converting enzyme) [424], PGR (progesterone receptor) [425], HPSE2 [426], LMTK3 [427], ALPP (alkaline phosphatase, placental) [428], EGFL6 [429], CACNA2D3 [430], MCTP1 [431], HKDC1 [432], S100A4 [433], MACC1 [434], MMP3 [435], FGFR2 [436], IL33 [437], FOXC2 [438], ITGA7 [439], EFEMP1 [440], GATA6 [441], BHLHE41 [442], TCF21 [443], GDF10 [444], NKX3-1 [445], AKR1C3 [446], SGK1 [447], RAP1GAP [448], FLI1 [449], NOX4 [450], SERPINE2 [451], IGSF9 [452], IGF2BP1 [453], SALL4 [454], VDR (vitamin D receptor) [455], CELSR2 [456],...…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

Vastrad,

Vastrad

2024

Preprint

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Endometriosis is a common cause of endometrial-type mucosa outside the uterine cavity with symptoms such as painful periods, chronic pelvic pain, pain with intercourse and infertility. However, the early diagnosis of endometriosis is still restricted. The purpose of this investigation is to identify and validate the key biomarkers of endometriosis. Next generation sequencing (NGS) dataset GSE243039 was obtained from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) between endometriosis and normal control samples were identified. After screening of DEGs, gene ontology (GO) and REACTOME pathway enrichment analyses were performed. Furthermore, a protein-protein interaction (PPI) network was constructed and modules were analysed using the Human Integrated Protein-Protein Interaction rEference (HIPIE) database and Cytoscape software, and hub genes were identified. Subsequantely, a network between miRNAs and hub genes, and network between TFss and hub genes were constructed using the miRNet and NetworkAnalyst tool, and possible key miRNAs and TFs were predicted. Finally, receiver operating characteristic curve (ROC) analysis was used to validate the hub genes. A total of 958 DEGs, including 479 up regulated genes and 479 down regulated genes, were screened between endometriosis and normal control samples. GO and REACTOME pathway enrichment analyses of the 958 DEGs showed that they were mainly involved in multicellular organismal process, developmental process, signaling by GPCR and muscle contraction. Further analysis of the PPI network and modules identified 10 hub genes, including VCAM1, SNCA, PRKCB, ADRB2, FOXQ1, MDFI, ACTBL2, PRKD1, DAPK1 and ACTC1. Possible target miRNAs, including hsa-mir-3143 and hsa-mir-2110, and target TFs, including TCF3 and CLOCK, were predicted by constructing a miRNA-hub gene regulatory network and TF-hub gene regulatory network. This investigation used bioinformatics techniques to explore the potential and novel biomarkers. These biomarkers might provide new ideas and methods for the early diagnosis, treatment, and monitoring of endometriosis.

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“…Interestingly, we identified an upregulated gene set responsible for lung development, which suggests that ZNF714 silencing may reduce cell potency. In our validation assays, we tested three downregulated genes with known oncogenic properties (Figure 6D), namely EFEMP2 [53,54], ITGA7 [55][56][57], and COL8A1 [58][59][60]. These genes participate in cell adhesion, extracellular matrix organization, cell proliferation, and anatomical structure morphogenesis (Figure 6B).…”

Section: Discussionmentioning

confidence: 99%

ZNF714 Supports Pro-Oncogenic Features in Lung Cancer Cells

Oleksiewicz,

Machnik,

Sobocińska

et al. 2023

IJMS

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Despite the ongoing progress in diagnosis and treatments, cancer remains a threat to more than one-third of the human population. The emerging data indicate that many Krüppel-associated box zinc finger proteins (KRAB-ZNF) belonging to a large gene family may be involved in carcinogenesis. Our previous study identified Zinc Finger Protein 714 (ZNF714), a KRAB-ZNF gene of unknown function, as being commonly overexpressed in many tumors, pointing to its hypothetical oncogenic role. Here, we harnessed The Cancer Genome Atlas (TCGA)-centered databases and performed functional studies with transcriptomic and methylomic profiling to explore ZNF714 function in cancer. Our pan-cancer analyses confirmed frequent ZNF714 overexpression in multiple tumors, possibly due to regional amplification, promoter hypomethylation, and Nuclear Transcription Factor Y Subunit Beta (NFYB) signaling. We also showed that ZNF714 expression correlates with tumor immunosuppressive features. The in vitro studies indicated that ZNF714 expression positively associates with proliferation, migration, and invasion. The transcriptomic analysis of ZNF714 knocked-down cells demonstrated deregulation of cell adhesion, migration, proliferation, apoptosis, and differentiation. Importantly, we provided evidence that ZNF714 negatively regulates the expression of several known TSGs indirectly via promoter methylation. However, as ZNF714 did not show nuclear localization in our research model, the regulatory mechanisms exerted by ZNF714 require further investigation. In conclusion, our results reveal, for the first time, that ZNF714 may support pro-oncogenic features in lung cancer cells.

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Effect of integrin α7 on cell proliferation, invasion, apoptosis and the PI3K/AKT pathway, and its association with clinicopathological features in endometrial cancer

Cited by 5 publications

References 28 publications

ITGA7 loss drives the differentiation of adipose‐derived mesenchymal stem cells to cancer‐associated fibroblasts

ITGA7 loss drives the differentiation of adipose‐derived mesenchymal stem cells to cancer‐associated fibroblasts

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

ZNF714 Supports Pro-Oncogenic Features in Lung Cancer Cells

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