Abstract:ABSTRACT. To examine the influence of insulin on megakaryocytopoiesis, we tested its effect on murine bone marrow cultures in a liquid culture system. In the presence of pokeweed mitogen-stimulated spleen cell conditioned medium in culture, insulin markedly enhanced megakaryocyte colony formation and increased the number and size of free megakaryocytes seen after 7 days. Many of the cells in cultures with insulin, however, were classified as immature, since they had a basophillic cytoplasm, a low cytoplasmic/n… Show more
“…In contrast to the ganglioside fraction, as shown in a previous study (21), insulin potentiated the increase in the number of megakaryocytes in immature state in vitro. In order to examine the interaction of insulin with the ganglioside fraction on murine megakaryocytopoiesis, both substances were applied to the liquid culture system.…”
Section: Resultssupporting
confidence: 46%
“…They suggested that 11-3 can support both megakaryocytic proliferation and maturation in vitro. In the present liquid culture system, more free megakaryocytes were produced than the cells in colonies, and the free mature cells which were stained intensely for acetylcholinesterase could be clearly distinguished from the free immature cells which were stained less intensely (21). The significant potentiation of the increase in the number of immature and mature megakaryocytes by the total ganglioside fraction suggests that the gangliosides play roles in both early and late megakaryocytopoiesis.…”
Section: Discussionmentioning
confidence: 64%
“…The presence of this cytoplasmic enzyme was used to identify megakaryocytes. The megakaryocytes in cultures were divided into two categories: 1) immature megakaryocytes which were stained less intensely, and 2) mature megakaryocytes which were stained intensely, according to the criterion described previously (21). The criterion for a megakaryocyte colony was the presence of four or more acetylcholinesterase-positive cells, which were in contact with each other.…”
ABSTRACT.Bovine brain gangliosides were applied to primary cultures of murine bone marrow cells to examine the role of gangliosides in development of megakaryocytes.Megakaryocytes in the cultures were detected by staining for a cytoplasmic enzyme, acetylcholinesterase, and divided into two types, 1)immature megakaryocytes which were stained less intensely, and 2) mature ones which were stained intensely. A medium containing total ganglioside fraction from bovine brain increased the number of both immature and mature megakaryocytes in the presence of pokeweed mitogen-stimulated murine spleen cell conditioned medium. Between the two cell types, the number of the mature cells was more significantly increased than the immature cells. The ganglioside GDla could substitute for the total ganglioside mixture. The results suggested that bovine brain gangliosides potentiated both megakaryocytic proliferation and maturation in vitro.
“…In contrast to the ganglioside fraction, as shown in a previous study (21), insulin potentiated the increase in the number of megakaryocytes in immature state in vitro. In order to examine the interaction of insulin with the ganglioside fraction on murine megakaryocytopoiesis, both substances were applied to the liquid culture system.…”
Section: Resultssupporting
confidence: 46%
“…They suggested that 11-3 can support both megakaryocytic proliferation and maturation in vitro. In the present liquid culture system, more free megakaryocytes were produced than the cells in colonies, and the free mature cells which were stained intensely for acetylcholinesterase could be clearly distinguished from the free immature cells which were stained less intensely (21). The significant potentiation of the increase in the number of immature and mature megakaryocytes by the total ganglioside fraction suggests that the gangliosides play roles in both early and late megakaryocytopoiesis.…”
Section: Discussionmentioning
confidence: 64%
“…The presence of this cytoplasmic enzyme was used to identify megakaryocytes. The megakaryocytes in cultures were divided into two categories: 1) immature megakaryocytes which were stained less intensely, and 2) mature megakaryocytes which were stained intensely, according to the criterion described previously (21). The criterion for a megakaryocyte colony was the presence of four or more acetylcholinesterase-positive cells, which were in contact with each other.…”
ABSTRACT.Bovine brain gangliosides were applied to primary cultures of murine bone marrow cells to examine the role of gangliosides in development of megakaryocytes.Megakaryocytes in the cultures were detected by staining for a cytoplasmic enzyme, acetylcholinesterase, and divided into two types, 1)immature megakaryocytes which were stained less intensely, and 2) mature ones which were stained intensely. A medium containing total ganglioside fraction from bovine brain increased the number of both immature and mature megakaryocytes in the presence of pokeweed mitogen-stimulated murine spleen cell conditioned medium. Between the two cell types, the number of the mature cells was more significantly increased than the immature cells. The ganglioside GDla could substitute for the total ganglioside mixture. The results suggested that bovine brain gangliosides potentiated both megakaryocytic proliferation and maturation in vitro.
“…Another postulated mechanism from a study in mice demonstrated that insulin causes megakaryocytes to produce larger platelets (29). Alternatively, increased platelet size may reflect the presence of high platelet turnover and younger platelets (30).…”
OBJECTIVEThe association between platelet activity, diabetes, and glucometabolic control is uncertain. We aim to investigate mean platelet volume (MPV), a marker of platelet size and platelet activity, with the prevalence of diabetes, metabolic syndrome, and degree of glycemic control.RESEARCH DESIGN AND METHODSThis is a retrospective analysis of 13,021 participants in the National Health and Nutrition Examination Survey from 1999 to 2004. Prevalence of diabetes was defined as nonfasting glucose >200 mg/dL, fasting glucose ≥126 mg/dL, or treatment with hypoglycemic agents. Presence of metabolic syndrome was determined by the National Cholesterol Education Program Adult Treatment Panel III definition. Odds ratios and 95% CIs were estimated by logistic regression.RESULTSMPV was significantly higher in subjects with diabetes (8.20 vs. 8.06 femtoliter [fL], P < 0.01) but not in subjects with metabolic syndrome (8.09 vs. 8.07 fL, P = 0.24). For the metabolic syndrome components, MPV was significantly higher in abdominal obesity (P = 0.03) and low HDL (P = 0.04), and not different in high blood pressure (P = 0.07), abnormal glucose metabolism (P = 0.71), or hypertriglyceridemia (P = 0.46). There was a significant correlation between MPV and glucose (P < 0.0001) and between MPV and hemoglobin A1c (P < 0.0001) in subjects with diabetes. These correlations were no longer significant in those without diabetes. The adjusted odds of diabetes rose with increasing MPV levels and were most pronounced in subjects with MPV levels exceeding the 90th percentile (≥9.31 fL). The association between MPV and diabetes was most apparent in those with the poorest glucose control.CONCLUSIONSMean platelet volume is strongly and independently associated with the presence and severity of diabetes.
“…In a study group of 22 patients with DM, MPV values were higher, but with the decrease in blood glucose, there was a significant decrease in MPV values [9]. It is suggested that insulin potentates murine megakaryocytopoiesis in vitro, in a study; therefore, due to the increased insulin in insulin resistant cases big platelets may be observed [16]. In another study, it is claimed that increased aggregation and multiplication functions occur in diabetic patients' megakaryocyte stem-cells.…”
There is a need for further research focusing on the platelet function in the observation and treatment of gestational diabetes, which can pose the risk of developing Type 2 diabetes for the mother and has negative consequences for the fetus.
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