Effect of insulin and 20-hydroxyecdysone in the fat body of the yellow fever mosquito, Aedes aegypti

Roy, Saurabh G.; Hansen, Immo A.; Raikhel, Alexander S.

doi:10.1016/j.ibmb.2007.08.004

Cited by 136 publications

(172 citation statements)

References 49 publications

(47 reference statements)

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“…The Ae. aegypti WT UGAL/Rockefeller strain and transgenic lines were reared at 27°C and 80% humidity, as described previously (28). Blood feeding of adult mosquitoes was performed using White Leghorn chickens.…”

Section: Methodsmentioning

confidence: 99%

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MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes

Lucas

Roy

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Female mosquitoes require a blood meal for reproduction, and this blood meal provides the underlying mechanism for the spread of many important vector-borne diseases in humans. A deeper understanding of the molecular mechanisms linked to mosquito blood meal processes and reproductive events is of particular importance for devising innovative vector control strategies. We found that the conserved microRNA miR-8 is an essential regulator of mosquito reproductive events. Two strategies to inhibit miR-8 function in vivo were used for functional characterization: systemic antagomir depletion and spatiotemporal inhibition using the miRNA sponge transgenic method in combination with the yeast transcriptional activator gal4 protein/upstream activating sequence system. Depletion of miR-8 in the female mosquito results in defects related to egg development and deposition. We used a multialgorithm approach for miRNA target prediction in mosquito 3′ UTRs and experimentally verified secreted winglessinteracting molecule (swim) as an authentic target of miR-8. Our findings demonstrate that miR-8 controls the activity of the longrange Wingless (Wg) signaling by regulating Swim expression in the female fat body. We discovered that the miR-8/Wg axis is critical for the proper secretion of lipophorin and vitellogenin by the fat body and subsequent accumulation of these yolk protein precursors by developing oocytes.small RNA | microRNA | Wingless signaling | reproduction | mosquito

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Section: Methodsmentioning

confidence: 99%

“…Mosquitoes were allowed to recover for 3-4 d before blood feeding. dsRNA was produced as described previously (28). In brief, dsRNA was synthesized using the MEGAscript kit (Ambion).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes

Lucas

Roy

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…The mosquito A. aegypti UGAL/Rockefeller strain was raised as described previously (21), and all animal experiments were performed with the approval of the Institutional Animal Use and Care Committee of the Korea Research Institute of Bioscience and Biotechnology.…”

Section: Methodsmentioning

confidence: 99%

Identification of plant compounds that disrupt the insect juvenile hormone receptor complex

Lee

Fāng

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. Insect-specific growth regulators represent attractive control agents because of their safety to the environment and humans. We identified plant compounds that serve as juvenile hormone antagonists (PJHANs). Using the yeast twohybrid system transformed with the mosquito JH receptor as a reporter system, we demonstrate that PJHANs affect the JH receptor, methoprene-tolerant (Met), by disrupting its complex with CYCLE or FISC, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants: Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD 50 values. Topical application of two diterpenes caused reduction in the expression of Met target genes and retardation of follicle development in mosquito ovaries. Hence, the newly discovered PJHANs may lead to development of a new class of safe and effective pesticides. Diseases transmitted by insects result in a million deaths per year (1), and insect infestation leads to annual losses of agricultural products worth billions of US dollars (2). The high toxicity of currently available insecticides presents environmental and health risks, and growing resistance and cross-resistance of insects to these existing insecticides gravely complicates the situation. Hence, there is an urgent need to develop novel effective insecticides.Insect growth regulators (IGRs) have been devised based on insect-specific functions. Three major classes of IGRs are commercially available (3). These IGRs include juvenile hormone (JH) agonists (methoprene and pyriproxyfen), ecdysone agonists (halofenozide and tebufenozide), and chitin synthase inhibitors (buprofezine). They possess low off-target toxicity and environmental danger and have been used to control pests. JHbased IGRs are of particular interest because JH is an insectspecific hormone. JH agonists (JHAs) disrupt insect endocrine regulation, causing abnormal development and larval fatality (4). However, JHAs have limitations in the scope of their applications because they mimic and enhance the JH mode of action. Theoretically, JH antagonists (JHANs) could be used as a powerful alternative, but no effective JHANs have yet been developed.Recent studies identified methoprene-tolerant (Met) as the JH receptor (5, 6). Met is a member of the family of basic helixloop-helix (bHLH)-Per-Arnt-Sim (PAS) transcription factors that requires homo-or heterodimerization for DNA binding and transcriptional regulation (7). In the Aedes aegypti mosquito, Met forms a heterodimer with other bHLH-PAS factors, the steroid receptor coactivator (SRC/FISC), or Cycle (CYC) in a JHdependent manner (8, 9). JH-mediated Met-CYC binding has been quantitatively simulated using the two-hybrid yeast β-galactosidase assay in a yeast cell, Y187 (9). Thus, it is possible to test both J...

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“…In addition, IIS is known to stimulate the secretion of ecdysteroid in follicle cells, resulting in vitellogenesis Brown, 1999, 2002;Roy et al 2007;Brown et al 2008). A similar mechanism would be expected to occur in adult reproductive differentiation in social insects.…”

Section: Introductionmentioning

confidence: 98%

“…Considering the roles of IIS in caste differentiation and its regulatory functions in the ovaries of solitary insects Brown, 1999, 2002;Wu and Brown, 2006;Roy et al, 2007;Brown et al, 2008), we hypothesized that the IIS pathway was involved in the control of ovarian activity in social insects as well. In order to 6 understand adult ovarian differentiation, we carried out histological examinations on ovarian changes and molecular analyses of IIS dynamics during reproductive differentiation in the queenless ponerine ant Diacamma sp.…”

Section: Introductionmentioning

confidence: 99%

Ovarian development and insulin-signaling pathways during reproductive differentiation in the queenless ponerine ant Diacamma sp.

Okada

Miyazaki

Miyakawa

et al. 2010

Journal of Insect Physiology

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Effect of insulin and 20-hydroxyecdysone in the fat body of the yellow fever mosquito, Aedes aegypti

Cited by 136 publications

References 49 publications

MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes

MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes

Identification of plant compounds that disrupt the insect juvenile hormone receptor complex

Ovarian development and insulin-signaling pathways during reproductive differentiation in the queenless ponerine ant Diacamma sp.

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