Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi

Juanssilfero, Ario Betha; Kahar, Prihardi; Amza, Rezky Lastinov; Miyamoto, Nao; Otsuka, Hiromi; Matsumoto, Hana; Thontowi, Ahmad; Yopi, -; Prasetya, Bambang; Kondo, Akihiko

doi:10.1016/j.jbiosc.2017.12.020

Cited by 83 publications

(37 citation statements)

References 35 publications

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“…2 ). However, it is still largely lower than the reported lipid accumulation potential of the strain (up to 85.10% g/g) [ 18 ]. The common explanation was that oleaginous yeast achieved high lipid accumulation in carbon-rich and nitrogen depletion conditions.…”

Section: Resultsmentioning

confidence: 88%

“…The lipid-producing strain was oleaginous yeast Lipomyces starkeyi which was purchased from China Center of Industrial Culture Collection (CICC). According to the reports, the highest lipid content in Lipomyces starkeyi obtained is 85.1% (w/w) [ 18 ]. The strain was preserved in 20% (w/w) glycerol at − 80 °C for long-term storage and revival was achieved by streaking onto a potato dextrose agar (PDA) plate [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

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Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

Chen

Zhang

et al. 2020

Biotechnol Biofuels

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Background Converting wastewater sludge to lipid is considered as one of the best strategies of sludge management. The current problem of lipid production from wastewater sludge is the low yield (0.10–0.16 g lipid/g dry sludge) due to the low availability of easily uptaken materials (such as soluble monosaccharide and oligosaccharide) in sludge to oleaginous microorganism (Rhodotorula glutinis, Trichosporon oleaginosus, Lipomyces starkeyi). Pretreatments are efficient methods to improve sludge bioavailability. This study is aimed to achieve high lipid production from sludge and high sludge reduction. Results In this study, it was observed that the soluble chemical oxygen demand (SCOD) had significantly increased after different pretreatment. The SCOD in the supernatant was increased from 32.64 to 180.25 mg/L, 924.16 mg/L, 1029.89 mg/L and 3708.31 mg/L after acidic (pH 2 for 2 h), alkaline (pH 12 for 2 h), microwave irradiation (15 min with 5 min interval), and ultrasonication (30 min at 450 W and 20 kHz frequency with 5 s on and 2 s off mode) pretreatment, respectively. Pretreatments have also increased the release of total nitrogen (TN) and total phosphorus (TP) from solids. The sludge after different pretreatments were used as a medium for lipid production, and the highest lipid content (36.67% g/g) was obtained in the fermentation with ultrasonication pretreatment sludge, and the sludge reduction was 63.10%. For other pretreatments, the lipid content and sludge reduction were 18.42% and 32.63% in acid pretreatment case, 21.08% and 36.44% in alkaline pretreatment case, and 26.31% and 43.03% in microwave pretreatment case, respectively. Conclusion It was found that ultrasonication pretreatment was the most efficient way to increase the sludge biodegradability (SCOD) and to release TN and TP from solid phase to liquid phase. Pretreated sludge for lipid production achieved significant improvement in lipid yield and sludge reduction. Lipids produced from pretreated sludge were transesterified to biodiesel and the analysis showed that biodiesel had a similar composition as commercial biodiesel. The study reveals that pretreatment on sludge is a promising method for enhancing biological sludge management efficiency.

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Section: Resultsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

Chen

Zhang

et al. 2020

Biotechnol Biofuels

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“…Cryptococcus curvatus , Cutaneotrichosporon oleaginosum ) maximum lipid accumulation occurred in batch culture with xylose as carbon source [ 36 ]. For Lipomyces starkeyi higher lipid contents were reached on xylose, yet on glucose a higher cell mass concentration was produced [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Gorte

Kugel

Ochsenreither

2020

Biotechnol Biofuels

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Background Biotechnologically produced microbial lipids are of interest as potential alternatives for crude and plant oils. Their lipid profile is similar to plant oils and can therefore be a substitute for the production of biofuels, additives for food and cosmetics industry as well as building blocks for oleochemicals. Commercial microbial lipids production, however, is still not profitable and research on process optimization and cost reduction is required. This study reports on the process optimization using glucose or xylose with the unconventional oleaginous yeast Saitozyma podzolica DSM 27192 aiming to reduce the applied carbon source amount without sacrificing lipid productivity. Results By optimizing the process parameters temperature and pH, lipid productivity was enhanced by 40%. Thereupon, by establishing a two-phase strategy with an initial batch phase and a subsequent fed-batch phase for lipid production in which a constant sugar concentration of about 10 g/L was maintained, resulted in saving of ~ 41% of total glucose and ~ 26% of total xylose. By performing the automated continuous sugar feed the total sugar uptake was improved to ~ 91% for glucose and ~ 92% for xylose and thus, prevented waste of unused carbon source in the cultivation medium. In addition, reduced glucose cultivation resulted in to 28% higher cell growth and 19% increase of lipid titer. By using xylose, the by-product xylonic acid was identified for the first time as by-product of S. podzolica. Conclusions These findings provide a broad view of different cultivation process strategies with subsequent comparison and evaluation for lipid production with S. podzolica. Additionally, new biotechnological characteristics of this yeast were highlighted regarding the ability to produce valuable organic acids from sustainable and renewable sugars.

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“…The lipid producing strain was oleaginous yeast Lipomyces starkeyi which was purchased from China Center of Industrial Culture Collection (CICC). According to the reports, the highest lipid content in Lipomyces starkeyi obtained is 85.1 % (w/w) (Juanssilfero et al 2018). The strain was preserved in 20% (w/w) glycerol at -80 °C for long term storage and revival was achieved by streaking onto a potato dextrose agar (PDA) plate (Juanssilfero et al 2018).…”

Section: Strainmentioning

confidence: 99%

“…According to the reports, the highest lipid content in Lipomyces starkeyi obtained is 85.1 % (w/w) (Juanssilfero et al 2018). The strain was preserved in 20% (w/w) glycerol at -80 °C for long term storage and revival was achieved by streaking onto a potato dextrose agar (PDA) plate (Juanssilfero et al 2018). For short term storage, the strain was maintained in malt extract agar plate and subculture was performed every 7 days.…”

Section: Strainmentioning

confidence: 99%

Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

Chen

Zhang

et al. 2020

Preprint

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Background: Converting wastewater sludge to lipid is considered as one of the best strategies of sludge management. The current problem of lipid production from wastewater sludge is the low yield (0.10-0.16 g lipid/g dry sludge) due to the low availability of easily uptaken materials (such as soluble monosaccharide and oligosaccharide) in sludge to oleaginous microorganism (Rhodotorula glutinis, Trichosporon oleaginosus, Lipomyces starkeyi). Pretreatments are efficient methods to improve sludge bioavailability. This study is aimed to achieve high lipid production from sludge and high sludge reduction. Results: In this study, it was observed that the soluble chemical oxygen demand (SCOD) had significantly increased after different pretreatment. The SCOD in the supernatant was increased from 32.64 to 180.25 mg/L, 924.16 mg/L, 1029.89mg/L and 3708.31 mg/L after acidic (pH 2 for 2 h), alkaline (pH 12 for 2 h), microwave irradiation (15 min with 5 min interval), and ultrasonication (30 min at 450 W and 20 kHz frequency with 5 s on and 2 s off mode) pretreatment, respectively. Pretreatments have also increased the release of total nitrogen (TN) and total phosphorus (TP) from solids. The sludge after different pretreatments were used as medium for lipid production, and the highest lipid content (36.67% g/g) was obtained in the fermentation with ultrasonication pretreatment sludge, and the sludge reduction was 63.10%. For other pretreatments, the lipid content and sludge reduction were 18.42% and 32.63% in acid pretreatment case, 21.08% and 36.44% in alkaline pretreatment case, and 26.31% and 43.03% in microwave pretreatment case, respectively.Conclusion: It was found that ultrasonication pretreatment was the most efficient way to increase the sludge biodegradability (SCOD) and to release TN and TP from solid phase to liquid phase. Pretreated sludge for lipid production achieved significant improvement in lipid yield and sludge reduction. Lipids produced from pretreated sludge were transesterified to biodiesel and the analysis showed that the biodiesel had a similar composition as commercial biodiesel. The study reveals that pretreatment on sludge is a promising method for enhancing biological sludge management efficiency.

show abstract

Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi

Cited by 83 publications

References 35 publications

Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding

Pretreatments for enhancing sewage sludge reduction and reuse in lipid production

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