Effect of Injection Speed on Oocyte Deformation in ICSI

Hajiyavand, Amir M.; Saadat, Mozafar; Abena, Alessandro; Sadak, Ferhat; Sun, Xiaochen

doi:10.3390/mi10040226

Cited by 16 publications

(10 citation statements)

References 28 publications

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“…As the algorithm had been validated, it was then used to evaluate the effect of egg-injection parameters such as speed on the vibration. In the previous publication, the authors demonstrated the variations in forces and deformation creation during egg injection [8]. In this research, the potential contribution of the demonstrated factors is evaluated in vibration creation which may be a cause of egg damage during the penetration.…”

Section: Resultsmentioning

confidence: 94%

“…Injection speed and trajectory of the injection pipette are two main injection parameters that contribute toward a successful egg injection [6]. The displacement of the injection pipette is affected significantly by cell injection speed and its trajectory [7,8]. Besides, the magnitude of the vibration at the tip of the injection pipette increases since the injection procedure requires acceleration and deceleration of the injection pipette [9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection

Sadak

Saadat

Hajiyavand

2019

Sensors

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Intracytoplasmic sperm injection (ICSI) is an infertility treatment where a single sperm is immobilised and injected into the egg using a glass injection pipette. Minimising vibration in three orthogonal axes is essential to have precise injector motion and full control during the egg injection procedure. Vibration displacement sensing using physical sensors in ICSI operation is challenging since the sensor interfacing is not practically feasible. This study proposes a non-invasive technique to measure the three-dimensional vibrational motion of the injection pipette by a single microscope camera during egg injection. The contrast-limited adaptive histogram equalization (CHALE) method and blob analyses technique were employed to measure the vibration displacement in axial and lateral axes, while the actual dimension of the focal axis was directly measured using the Brenner gradient algorithm as a focus measurement algorithm. The proposed algorithm operates between the magnifications range of 4× to 40× with a resolution of half a pixel. Experiments using the proposed vision-based algorithm were conducted to measure and verify the vibration displacement in axial and lateral axes at various magnifications. The results were compared against manual procedures and the differences in measurements were up to 2% among all magnifications. Additionally, the effect of injection speed on lateral vibration displacement was measured experimentally and was used to determine the values for egg deformation, force fluctuation, and penetration force. It was shown that increases in injection speed significantly increases the lateral vibration displacement of the injection pipette by as much as 54%. It has been demonstrated successfully that visual sensing has played a key role in identifying the limitation of the egg injection speed created by lateral vibration displacement of the injection pipette tip.

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection

Sadak

Saadat

Hajiyavand

2019

Sensors

Self Cite

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“…Oocyte morphological assessment is the most widely used method in clinical practice; a normal human MII oocyte should have a round and clear ZP, a small PVS containing a single IPB without fragmentation and pale, moderately granular cytoplasm with no inclusions ( 18 ). However, oocyte morphological assessment may be performed only after oocyte denudation in ICSI cases and potential mechanisms that change the appearance of the oocyte are multifactorial ( 19 ). Age and the E 2 level on the HCG day are indirect indicators ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

Cystic fibrosis transmembrane conductance regulator in follicular fluid and cumulus cells and its relationship with age

Wang

Wei

et al. 2020

Exp Ther Med

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Oocyte quality deteriorates with female age and numerous indicators of oocyte quality exist. In the present study, the levels of cystic fibrosis transmembrane conductance regulator (CFTR) in the follicular fluid (FF) and cumulus cells (CCs) of infertile females in 3 different age groups were assessed to determine the relationship between CFTR and female age. The general features of the 3 groups, including age, body mass index, infertility duration, basal hormone levels and the number of retrieved oocytes were compared. The FF CFTR levels of the 3 groups were also compared and multiple age-related indicators of oocyte quality were analyzed, including the estradiol levels on the human chorionic gonadotropin injection day, the morphologically normal oocyte rate and the available or high-quality embryo rate. Immunofluorescence and PCR analyses were performed to examine CFTR expression in CCs around oocytes. The results indicated differences in general features and several indicators of oocyte quality among the 3 groups and significant differences in CFTR. The FF CFTR level was positively correlated with age, which was confirmed by immunofluorescence and PCR. Collectively, these results indicated that CFTR expression in FF and CCs may be associated with oocyte quality based on the age of individuals undergoing the assisted reproduction technique.

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“…For this reason, an optimum range of injection speeds has to be determined. An automated system should be able to determine the speed and force of injection based on the biophysical properties of the oocyte (103).…”

Section: Oocytesmentioning

confidence: 99%

Are we approaching automated assisted reproductive technology? Sperm analysis, oocyte manipulation, and insemination

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

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Assisted reproductive technology (ART) procedures are mostly performed manually and require meticulous attention to details and precision in handling and timing. Automation of ART procedures would allow achieving standardization and reducing performance variability, the cost of medical treatment, and the risk of human error. Some ART diagnostic devices are already available and offer objective tools of evaluation. However, automation of the entire spectrum of ART procedures is yet to come and can only be imagined as a platform capable of integrating all the separate technologies, successfully interconnecting them to guarantee a continued chain of custody of the gametes and embryos. The present update seeks to review the current potential for automation within the in vitro fertilization laboratory, with attention to sperm and oocyte manipulation and selection and to oocyte insemination with standard in vitro fertilization or intracytoplasmic sperm injection. An electronic search of PubMed was performed to identify articles in English that addressed automation in ART. Studies were classified in categories as randomized controlled trials, prospective controlled trials, prospective noncontrolled trials, retrospective studies, and experimental studies. Research and development data from the authors are included.

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Effect of Injection Speed on Oocyte Deformation in ICSI

Cited by 16 publications

References 28 publications

Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection

Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection

Cystic fibrosis transmembrane conductance regulator in follicular fluid and cumulus cells and its relationship with age

Are we approaching automated assisted reproductive technology? Sperm analysis, oocyte manipulation, and insemination

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