Effect of Inhibiting p38 on HuR Involving in β-AChR Post-transcriptional Mechanisms in Denervated Skeletal Muscle

Wang, Hong; Zhao, Xiao Li; Wang, Yun; Chen, Lianhua; Li, Shitong

doi:10.1007/s10571-019-00698-0

Cited by 3 publications

(4 citation statements)

References 20 publications

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“…Studies have revealed that HuR is closely related to the occurrence and development of inflammatory diseases and tumors, such as cell division, cell senescence, immune cell activation, and other life activities 22,23 . HuR regulates its stability and translation efficiency by specifically binding to target mRNA, thereby affecting the expression level of target genes in cells 24,25 . In the present study, we proved the interaction between circ0104103/LACTB and HuR by performing RNA pull‐down and RIP assays.…”

Section: Discussionmentioning

confidence: 51%

“… 22 , 23 HuR regulates its stability and translation efficiency by specifically binding to target mRNA, thereby affecting the expression level of target genes in cells. 24 , 25 In the present study, we proved the interaction between circ0104103/LACTB and HuR by performing RNA pull‐down and RIP assays. Rescue experiments demonstrated that HuR facilitates its stability by binding to LACTB mRNA and thus regulating its protein level expression in CRC cells.…”

Section: Discussionmentioning

confidence: 60%

“…22,23 HuR regulates its stability and translation efficiency by specifically binding to target mRNA, thereby affecting the expression level of target genes in cells. 24,25 In the present study, we proved the interaction between circ0104103/LACTB and HuR by performing RNA Recently, an increasing number of studies have shown that cir-cRNAs function as competitive inhibitors of microRNAs (miRNAs) and then regulate their expression to participate in various cancer biological processes. 26 miRNAs are a large class of short (~22 nt) noncoding RNAs that are closely associated with cancer progression.…”

Section: Tumor-suppressing Functions Of Circ0104103 Are Dependent On ...mentioning

confidence: 54%

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Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR‐373‐5p

Tan

Sun

et al. 2023

Cancer Science

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Emerging evidence has suggested that circular RNAs (circRNAs) have vital functions during the initiation and progression of various diseases. However, circRNA potential mechanisms in colorectal cancer (CRC) are largely unknown. Here, we sought to investigate the role and underlying regulatory mechanism of circ0104103 in CRC. circ0104103 was validated by quantitative RT‐PCR (qRT‐PCR) and Sanger sequencing. Gain‐ and loss‐of‐function assays in cell lines and mouse xenograft models were utilized to investigate the effects of circ0104103 in CRC. RNA pull‐down assays, RNA immunoprecipitation assays, bioinformatics analyses, RNA FISH, and luciferase reporter assays were used to elucidate the potential mechanism of circ0104103 in CRC. We identified circ0104103, which is strongly downregulated in CRC tissues and cell lines. Functional studies revealed that circ0104103 inhibited CRC cell growth, migration, and invasion both in vitro and in vivo. Mechanistically, circ0104103 binds to HuR, a functional RNA‐binding protein commonly expressed in CRC. HuR binds to the 3′UTR of LACTB mRNA to facilitate stabilization and increase its expression. Moreover, circ0104103 was verified as a competing endogenous RNA (ceRNA) via negative regulation of miR‐373‐5p to increase LACTB expression, resulting in inhibiting the occurrence and progression of CRC. Taken together, our study revealed that circ0104103 acts as a tumor suppressor and may be a novel biomarker and therapeutic target in CRC.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 60%

“…22,23 HuR regulates its stability and translation efficiency by specifically binding to target mRNA, thereby affecting the expression level of target genes in cells. 24,25 In the present study, we proved the interaction between circ0104103/LACTB and HuR by performing RNA Recently, an increasing number of studies have shown that cir-cRNAs function as competitive inhibitors of microRNAs (miRNAs) and then regulate their expression to participate in various cancer biological processes. 26 miRNAs are a large class of short (~22 nt) noncoding RNAs that are closely associated with cancer progression.…”

Section: Tumor-suppressing Functions Of Circ0104103 Are Dependent On ...mentioning

confidence: 54%

See 1 more Smart Citation

Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR‐373‐5p

Tan

Sun

et al. 2023

Cancer Science

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“…demonstrated that knockdown of HuR could restrain the bone metastasis and osteolysis in mice with metastatic breast cancer (Lee et al., 2017 ). Another study revealed that proper exercise modulates HuR expression in aging skeletal muscle, thereby stabilizing various mRNAs and delaying skeletal muscle atrophy (Cammas et al., 2014 ; Wang et al., 2019 ). Moreover, our recent study identified HuR as an essential osteoporosis‐related RBP (Huai et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

HuR‐positive stress granules: Potential targets for age‐related osteoporosis

Huai,

Wang,

Mao

et al. 2024

Aging Cell

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Aging impairs osteoblast function and bone turnover, resulting in age‐related bone degeneration. Stress granules (SGs) are membrane‐less organelles that assemble in response to stress via the recruitment of RNA‐binding proteins (RBPs), and have emerged as a novel mechanism in age‐related diseases. Here, we identified HuR as a bone‐related RBP that aggregated into SGs and facilitated osteogenesis during aging. HuR‐positive SG formation increased during osteoblast differentiation, and HuR overexpression mitigated the reduction in SG formation observed in senescent osteoblasts. Moreover, HuR positively regulated the mRNA stability and expression of its target β‐catenin by binding and recruiting β‐catenin into SGs. As a potential therapeutic target, HuR activator apigenin (API) enhanced its expression and thus aided osteoblasts differentiation. API treatment increased HuR nuclear export, enhanced the recruitment of β‐catenin into HuR‐positive SGs, facilitated β‐catenin nuclear translocation, and contributed osteogenesis. Our findings highlight the roles of HuR and its SGs in promoting osteogenesis during skeletal aging and lay the groundwork for novel therapeutic strategies against age‐related skeletal disorders.

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A novel CARM1–HuR axis involved in muscle differentiation and plasticity misregulated in spinal muscular atrophy

Ravel‐Chapuis

Haghandish

Daneshvar

et al. 2021

Human Molecular Genetics

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Spinal muscular atrophy (SMA) is characterized by the loss of alpha motor neurons in the spinal cord and a progressive muscle weakness and atrophy. SMA is caused by loss-of-function mutations and/or deletions in the survival of motor neuron (SMN) gene. The role of SMN in motor neurons has been extensively studied, but its function and the consequences of its loss in muscle has also emerged as a key aspect of SMA pathology. In this study, we explore the molecular mechanisms involved in muscle defects in SMA. First, we show in C2C12 myoblasts, that arginine methylation by CARM1 controls myogenic differentiation. More specifically, the methylation of HuR on K217 regulates HuR levels and subcellular localization during myogenic differentiation, and the formation of myotubes. Furthermore, we demonstrate that SMN and HuR interact in C2C12 myoblasts. Interestingly, the SMA-causing E134K point mutation within the SMN Tudor domain, and CARM1 depletion, modulate the SMN-HuR interaction. In addition, using the Smn2B/− mouse model, we report that CARM1 levels are markedly increased in SMA muscles and that HuR fails to properly respond to muscle denervation, thereby affecting the regulation of its mRNA targets. Altogether, our results show a novel CARM1-HuR axis in the regulation of muscle differentiation and plasticity as well as in the aberrant regulation of this axis caused by the absence of SMN in SMA muscle. With the recent developments of therapeutics targeting motor neurons, this study further indicates the need for more global therapeutic approaches for SMA.

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Effect of Inhibiting p38 on HuR Involving in β-AChR Post-transcriptional Mechanisms in Denervated Skeletal Muscle

Cited by 3 publications

References 20 publications

Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR‐373‐5p

Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR‐373‐5p

HuR‐positive stress granules: Potential targets for age‐related osteoporosis

A novel CARM1–HuR axis involved in muscle differentiation and plasticity misregulated in spinal muscular atrophy

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