Effect of inhibin immunisation using different synthetic peptide fragments of the bovine αc-subunit on plasma anti-inhibin titres, plasma FSH concentrations and the incidence of multiple ovulation in heifers

Bleach, E.C.L.; Muttukrishna, S; Cunningham, F. J.; Knight, P. G.; Glencross, R. G.

doi:10.1016/0378-4320(95)01436-5

Cited by 18 publications

(11 citation statements)

References 15 publications

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“…The results confirm and extend previous evidence (Knight et al 1991b, Fray et al 1994, Bleach et al 1996 suggesting that the long-term stimulatory effect of IMM on follicle development may not be due exclusively to neutralization of the negative feedback action of inhibin on pituitary FSH. This contrasts with the acute increase in FSH secretion observed after passive immunization against inhibin (Mann et al 1989, 1993 where the associated increase in follicular development can be directly attributed to the FSH rise.…”

Section: Discussionsupporting

confidence: 89%

Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A, inhibin A and follistatin

Tannetta

Feist²,

Bleach³

et al. 1998

Journal of Endocrinology

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Active immunization of ewes against inhibin (IMM) consistently increases ovulation rate but this response is not always accompanied by the expected rise in plasma FSH. Inhibin-related molecules also have local auto/paracrine effects within the ovary and the ovulatory response to IMM could be due to neutralization of one of these effects, independent of changing FSH levels. To investigate this, ovaries were collected from long-term IMM (n=6) and control (CON; n=8) ewes killed 48 h after progestagen withdrawal (late follicular phase) and all follicles _3 mm were recovered to determine intrafollicular levels of inhibin A, activin A and follistatin by specific two-site immunoassay and oestradiol and testosterone by radioimmunoassay. Blood samples were collected to assess plasma FSH, oestradiol and inhibin antibody titres. Although plasma FSH levels were similar in IMM and CON ewes, IMM ewes had 3-fold more follicles _3 mm (P<0·0001) and 3-fold more oestrogenic follicles (P<0·001) than CON ewes. Compared with CON ewes, follicles from IMM ewes had much higher concentrations of activin A (6-fold; P<0·001) and inhibin A (3-fold; P<0·001) but only slightly more follistatin (1·4-fold; not significant). The activin A:follistatin ratio in follicles from IMM ewes (1:1) was significantly higher (P<0·001) than in follicles from CON ewes (0·3:1). Levels of inhibin antibody measured in follicular fluid (FF) from IMM ewes were similar to plasma levels. Given that activin A has been shown previously to up-regulate FSH receptors and aromatase activity in rat granulosa cells, the increase in intrafollicular activin A, unaccompanied by a rise in the concentration of its binding protein (follistatin), could explain how long-term IMM enhances follicle development and ovulation rate without necessarily promoting a sustained increase in FSH secretion.

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Section: Discussionsupporting

confidence: 89%

Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A, inhibin A and follistatin

Tannetta

Feist²,

Bleach³

et al. 1998

Journal of Endocrinology

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“…Considering the FSH profile in the present and previous [32] studies, a persistence of increased FSH levels is likely to be involved in the maintenance or stimulation of growth of large follicles. On the other hand, recent results obtained from active immunization [3,24] or repeated injections of inhibin antiserum [5,6] suggest that ovarian response to inhibin immunization is not only mediated by the rise in circulating FSH levels but also by neutralization of local actions of inhibin on follicular growth. The mature form of inhibin (32 kDa) enhanced thecal androgen production [11,35] while suppressing estradiol production in granulosa cells [36] in vitro, and precursors of α-inhibin seem to act as a competitor of FSH receptors in granulosa cells [30].…”

Section: Discussionmentioning

confidence: 98%

Ovarian Response and FSH Profile in Cows Following Injection of Various Doses of Inhibin Antiserum.

Akagi¹,

Kaneko

Nakanishi³

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Dose effect of inhibin antiserum on ovarian response and hormonal profiles were investigated. On day 12 of the estrous cycle (day 0=estrus), 14 of 19 cows were given a single i.v. injection of 25 ml (n=4), 37.5 ml (n=5) or 50 ml (n=5) antiserum against inhibin produced in a castrated male goat. The other 5 animals were given 50 ml castrated male goat serum (control serum). The animals in each group received a single i.m. injection of 0.5 mg prostaglandin F2α analogue (PG) 48 hr following the serum injection. The population of follicles and ovulation rate (estimated by the number of corpora lutea) were examined by ultrasonography. Administration of inhibin antiserum consistently resulted in a significant (p<0.01) increase in plasma concentrations of follicle-stimulating hormone (FSH) in inhibin-neutralized groups, although the increased FSH levels were sustained longer in 50-ml group than in the 25-and 37.5-ml groups. Levels in the circulating inhibin antibody titer were positively correlated with dosage of inhibin antiserum. A large number of antral follicles (≥ 4 mm in diameter) developed similarly after hypersecretion of FSH in all neutralized groups, coupled with a rise in plasma estradiol levels, while the number of large follicles (≥ 10 mm in diameter) on estrus showed a dose-dependent increase. Multiple ovulation (2 to 4) was recorded in all animals after injection of 50 ml inhibin antiserum, however all cows in the 25-ml group experienced only one ovulation and injection of 37.5 ml resulted in a variable number of ovulations (1 to 5). These results demonstrated that administration of inhibin antiserum on day 12, followed by injection of PG, was able to induce hypersecretion of FSH and subsequently multiple ovulations. The number of large follicles on estrus day and ovulations were affected by dosage of inhibin antiserum and were correlated with persistence of increased FSH levels or circulating antibody levels. -KEY WORDS: cattle, follicular growth, FSH, inhibin (passive immunization), ovulation rate.J. Vet. Med. Sci. 59(12): 1129-1135 inhibin binding or subsequent ovulation rate has not been fully demonstrated. In this study, using various doses of inhibin antiserum, we determined changes in plasma FSH levels and the number of ovulation or large follicles, to examine the relationship between FSH profile and ovarian response. MATERIALS AND METHODSExperimental design: Eight Japanese black, nine Japanese brown and two Japanese black × Japanese brown cattle, clinically normal with regular estrous cycle, were used. Animals were randomly divided into four groups. On day 12 of the estrous cycle (day 0=estrus), 14 of 19 cows were given a single i.v. injection of 25 ml (n=4; 2 Japanese black and 2 Japanese brown), 37.5 ml (n=5; 2 Japanese black and 3 Japanese brown) or 50 ml (n=5; 2 Japanese black, 2 Japanese brown and 1 Japanese black × Japanese brown) of inhibin antiserum. The other 5 animals (2 Japanese Black, 2 Japanese Brown and 1 Japanese black × Japanese brown) were given 50 ml castrated male goa...

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“…This action, timed to coincide with PGF2α injection in the present study, enlarged the pool of recruitable follicles, and this gave rise to a greater ovulation rate in the immunized goats. Previous results obtained from active immunization [47] or repeated injections of inhibin antiserum [8,48] have indicated a possibility that ovarian response to inhibin immunization is not only mediated by the rise in circulating FSH levels but is also mediated by the direct effect of immunization on follicular growth probably through local effects of inhibins in the ovary. Regarding LH levels, there was no marked change in the basal LH concentrations between the immunized and control goats.…”

Section: Discussionmentioning

confidence: 99%

Immunization of Goats against Inhibin Increased Follicular Development and Ovulation Rate

Sasaki

Medan

Watanabe

et al. 2006

Journal of Reproduction and Development

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Abstract. In the present study, two experiments were conducted to induce superovulation in goats using passive and active immunization against inhibin. In the first experiment, two groups of goats were given an intravenous injection of either 10 ml normal goat serum (control; n=6) or inhibin antiserum developed against [Tyro 30 ]-inhibin α (1-30) (passively immunized; n=6) 48 h before treatment with PGF2α. In the second experiment, two groups of goats were immunized with inhibin vaccine (actively immunized; n=5) or Freund's adjuvant (control; n=5) followed by three booster immunizations at 4 week intervals. Blood samples were collected for determination of FSH, LH, estradiol-17β, and progesterone. Ultrasonography was used to determine ovarian activity at PGF2α injection and ovulation rate one week after estrus. In both experiments, there was a significant increase in plasma FSH concentration compared with the controls. However, the pattern of the FSH levels was different between the passively and actively immunized goats. The numbers of follicles in passively and actively immunized goats (22.4 ± 2.3 and 18.6 ± 2.1, respectively) were significantly greater than those in the controls (2.6 ± 0.4 and 2.3 ± 0.4, respectively). In addition, the ovulation rate was greater in the immunized animals compared with the controls. Therefore, either passive or active immunization against inhibin could be used to induce superovulation in goats. Key words: Goats, Inhibin, Ovulation rate, Passive and active immunization (J. Reprod. Dev. 52: [543][544][545][546][547][548][549][550] 2006) nhibin is a heterodimeric glycoprotein hormone that selectively inhibits secretion of follicle stimulating hormone (FSH) from the pituitary gland [1]. A negative relationship between plasma concentrations of FSH and inhibin has been established in several mammalian species [2,3]. The number of follicles that develop to ovulatory size depends on both the amount of FSH and the time of exposure to FSH [4,5]. The release of FSH by the pituitary is in turn controlled by synergistic action of two of the major products of ovulatory follicles, inhibin and estradiol [6,7]. Previous findings [8][9][10] that immunoneutralization of endogenous inhibin produced a significant elevation of peripheral FSH offer evidence that inhibin is an important factor in the inhibitory regulation of FSH secretion in domestic animals as well as laboratory animals [11][12][13]. On the other hand, ultrasonographic observation of the ovary

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Effect of inhibin immunisation using different synthetic peptide fragments of the bovine αc-subunit on plasma anti-inhibin titres, plasma FSH concentrations and the incidence of multiple ovulation in heifers

Cited by 18 publications

References 15 publications

Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A, inhibin A and follistatin

Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A, inhibin A and follistatin

Ovarian Response and FSH Profile in Cows Following Injection of Various Doses of Inhibin Antiserum.

Immunization of Goats against Inhibin Increased Follicular Development and Ovulation Rate

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