“…Then, 60 leaves (10 leavesˆ2 accessionsˆ3 treatments) were randomly collected to evaluate the stomatal characteristics, both frequency (stomata mm´2) and size (width and length), following the technique reported by Camposeo et al [18] using light microscopy (Nikon Instruments Europe, Florence, Italy) and the image analysis software ARKON (version Arkon 32, Nikon Instruments, Florence, Italy). At the same time, on fully expanded, healthy, well light-exposed leaves [19], stomatal conductance (mmol H 2 O m´2¨s´1), transpiration rate (T, mmol H 2 O m´2¨s´1; data not shown) and net assimilation rate (A, µmol CO 2 m´2¨s´1) using the open system gas exchange analyzer LCA-4 (Leaf chamber Analyzer, ADC Ltd., Hoddeson, UK) with PLC (Parkinson Leaf chamber) B-002 (Analytical Development Company, Hoddesdon, Herts, UK), were determined under saturated photosynthetically active radiation (PAR > 1700 µmol m´2¨s´1).…”