Effect of in vitro inorganic lead on dopamine release from superfused rat striatal synaptosomes

Minnema, Daniel J.; Greenland, Robert D.; Michaelson, I.Arthur

doi:10.1016/0041-008x(86)90148-1

Cited by 71 publications

(20 citation statements)

References 30 publications

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“…Lead has been shown to alter the turnover or release DA (Minnema et al, 1986;Cory-Slechta, 1995;Kala and Jadhav, 1995a) and glutamate (Braga et al, 1999;Lasley and Gilbert, 2002), both of which are required for the amphetamine-induced activation of striatal cFOS (Berretta et al, 1992;Kiba and Jayaraman, 1994;Liu et al, 1994). It has been reported that exposure to 50 ppm lead for 90 days resulted in a decrease in DA release in the nucleus accumbens (Kala and Jadhav, 1995a).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have shown that lead exposure alters the turnover or release of many classical neurotransmitters, including glutamate, acetylcholine, GABA, and DA (Minnema et al, 1986;Shao and Suszkiw, 1991;Cory-Slechta, 1995;Kala and Jadhav, 1995a,b;Braga et al, 1999;Lasley and Gilbert, 2002). The present study focuses on the effects of in vivo lead exposure on the midbrain DA system.…”

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confidence: 99%

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Inorganic Lead Exposure in the Rat Activates Striatal cFOS Expression at Lower Blood Levels and Inhibits Amphetamine-Induced cFOS Expression at Higher Blood Levels

Lewis

Pitts

2004

J Pharmacol Exp Ther

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The impact of inorganic lead exposure on dopamine (DA) neurotransmission in the basal ganglia was examined. Amphetamine (AMPH)-induced cFOS immunoreactivity (cFOS-IR) in the striatum was determined after a 3-week exposure to lead acetate (0, 50, or 250 ppm). On the 21st day of lead exposure, rats were challenged with AMPH (4 mg/kg i.p.) or saline vehicle (Veh) and were assayed for presence of cFOS-IR. In the untreated control (Con) group, AMPH challenge (Con/AMPH) increased cFOS-IR expression by approximately 35-fold over Veh challenge (Con/Veh) (P Ͻ 0.01). In the Pb50/Veh group, cFOS-IR expression was approximately 7-fold greater than in the Con/Veh group (P Ͻ 0.05). Given that there was negligible cFOS-IR expression in the Con/Veh group, this indicates that the Pb50 exposure induced cFOS expression. The increase in cFOS-IR in the Pb50/AMPH was also significant (P Ͻ 0.01), but it was not different from the Con/AMPH (P Ͼ 0.20). Neither the Pb250/Veh group nor the Pb250/AMPH group had a significant increase in cFOS-IR relative to Con/Veh (P Ͼ 0.20). These results indicate that chronic 50 ppm lead exposure induced a low but statistically significantly level of cFOS gene activation and that it did not affect the AMPH-induced cFOS activation. However, chronic 250 ppm lead exposure inhibited AMPHinduced activation of cFOS in the striatum by about 89%. Therefore, lead is capable of both activating cFOS expression at low levels of exposure (mean blood lead level 21.6 Ϯ 1.9 g/dl) and inhibiting AMPH-induced cFOS expression at higher levels of exposure (mean blood lead level 47.4 Ϯ 2.6 g/dl).

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Inorganic Lead Exposure in the Rat Activates Striatal cFOS Expression at Lower Blood Levels and Inhibits Amphetamine-Induced cFOS Expression at Higher Blood Levels

Lewis

Pitts

2004

J Pharmacol Exp Ther

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“…Additional evidence that lead can inhibit calcium channels comes from studies on excitable cells from a variety of species: rat striatal synaptosomes (84); neurons in the pond snail (85,86); in neuroblastoma cells (87); in the abdominal ganglion of a marine mollusk (88)(89)(90); and in cultures of dorsal root ganglion cells of the rat (91). Not all findings are identical in detail, for example, irreversible effects of lead have been noted.…”

Section: Leadmentioning

confidence: 99%

Molecular and Ionic Mimicry of Toxic Metals

Clarkson¹

1993

Annu. Rev. Pharmacol. Toxicol.

181

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“…Neurotransmission during the prenatal/early childhood period is a signaling process which determines synaptic and neuronal pruning (37). Lead interferes with this process by both inhibiting depolarization by blocking Ca++ channels and by stimulating neurotransmitter release in the absence of an environmental cue (38)(39)(40)(41)(42). Moreover, lead, cadmium, arsenic and manganese have all been demonstrated to inhibit depolarization-evoked neurotransmitter release.…”

Section: Early Life Programming Of Neurodevelopmentmentioning

confidence: 99%

The Challenge Posed to Children's Health by Mixtures of Toxic Waste: The Tar Creek Superfund Site as a Case-Study

Shine²,

Wright

2007

Pediatric Clinics of North America

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Effect of in vitro inorganic lead on dopamine release from superfused rat striatal synaptosomes

Cited by 71 publications

References 30 publications

Inorganic Lead Exposure in the Rat Activates Striatal cFOS Expression at Lower Blood Levels and Inhibits Amphetamine-Induced cFOS Expression at Higher Blood Levels

Inorganic Lead Exposure in the Rat Activates Striatal cFOS Expression at Lower Blood Levels and Inhibits Amphetamine-Induced cFOS Expression at Higher Blood Levels

Molecular and Ionic Mimicry of Toxic Metals

The Challenge Posed to Children's Health by Mixtures of Toxic Waste: The Tar Creek Superfund Site as a Case-Study

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