Effect of in ovo injection of cadmium on chicken embryo heart

Pawlak, Krzysztof; Dugan, Magorzta; Wojtysiak, Dorota; Lis, Marcin; Niedzioka, Jerzy

doi:10.5897/ajar12.2116

Cited by 10 publications

(1 citation statement)

References 31 publications

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“…Cadmium chloride (SIGMA-ALDRICH), was used as source of cadmium in the study. A sublethal dose of cadmium (Dżugan et al, 2011) was prepared in sterilized 0.7% avian saline (Pawlak et al, 2013) in such a way that each 0.05ml contained 1.5 µg of cadmium.…”

Section: Chemical Used and Dose Preparationmentioning

confidence: 99%

Protection Against Cadmium-Induced Abnormalities and Hepatotoxicity in ovo by Allium sativum

Andleeb¹,

Shaukat²,

Ara³

2018

Punjab Univ. J. Zool.

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| Cadmium (Cd), a toxic heavy metal, is a potential carcinogen, mutagen and teratogen through bioaccumulation in tissues. Aim of present study was to analyze various developmental abnormalities by a sub-lethal dose of cadmium chloride and protective role of garlic (Allium sativum), to minimize the intensity of these toxicities. For this purpose, fertilized eggs of Gallus domesticus were randomly divided into four groups of forty eggs each. Control group was intact and untreated. Eggs of one group were injected with a sub-lethal dose of cadmium chloride (1.5 µg/egg) in albumin on 7 th day of incubation. In another group, eggs were treated with cadmium chloride and fresh garlic juice (0.2 µg / egg) after a short interval of 10 minutes. Antidote group was injected with fresh garlic juice (0.2 µg / egg). Eggs of each group were incubated at 37 ± 0.5 °C and at 50-60% relative humidity till hatching. Natural hatching was obtained as 92.5, 32.5, 72.5, and 87.5% in control, dose, dose+antidote, and anitodote groups respectively with delayed hatching in some cases in dose and dose+antidote groups. Chicks and dead embryos were studied for morphological and morphometric analysis. Liver tissues were dissected out from each group for histological analysis. Cadmium chloride induced significant developmental abnormalities such as reduced body weight and crown rump length, exencephaly, ablepharia, crossed beak, gastroschisis, crooked toes, non-alcoholic fatty liver and pyknosis. Fresh garlic juice has successfully attenuated these toxicities and increased the body weight, crown rump length. Results of present study determine that garlic is a potential antidote to ameliorate the cadmium induced teratogenic and hepatotoxic defects in developing Gallus domesticus.

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Section: Chemical Used and Dose Preparationmentioning

confidence: 99%

Protection Against Cadmium-Induced Abnormalities and Hepatotoxicity in ovo by Allium sativum

Andleeb¹,

Shaukat²,

Ara³

2018

Punjab Univ. J. Zool.

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Cardiotoxicity of Cadmium and Its Effects on Heart Efficiency During Early and Late Chick Embryogenesis

Ali,

Awadalla,

Hamed

et al. 2024

Cardiovasc Toxicol

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Cadmium (Cd) is a dangerous heavy metal that is non-degradable in the environment. Many organs can accumulate Cd and adversely affect organ function and health. Cd is considered as a teratogenic and embryotoxic agent. This study aims to evaluate the teratogenicity of Cd at concentrations lesser than the permissible and its effects on the heart during chick embryogenesis. Fertilized eggs of the chick Gallus domesticus were divided into; control, saline injected and four experimental groups injected with single doses of 5, 25, 50 or 75 µM of CdCl2. Histological observations of the heart before hatching and the cardiomyocytes after hatching were recorded. Morphometric measurements of heart chambers were achieved at 3, 4 and 6 days of incubation. Electrocardiograph and respiratory rate were recorded at tenth day. Different cardiac problems had been brought on by Cd. In comparison to controls, the heart looked much larger, and in certain cases, growth retardation was seen. Degeneration in heart walls and malformations of dorsal aorta were noticed. Morphometrically, the width and wall thickness of heart chambers showed significant changes. Heart beats and respiratory rate significantly decreased compared to control. The cardiotoxic effect of Cd on heart compartments structure and function was dose dependent. One of Cd toxicity is its ability to induce cellular oxidative stress. The heart in particular is sensitive to oxidative stress. Cardiac oxidative stress might intensify heart failure and promote disease progression. Calcium is one of the components that is needed for normal heart work. Cd might interfere with calcium metabolism by removing it from the body.

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Cadmium induced cardiac toxicology in developing Japanese quail (Coturnix japonica): Histopathological damages, oxidative stress and myocardial muscle fiber formation disorder

Zheng

Zhang

et al. 2021

Comparative Biochemistry and Physiology Part C: Toxicology &

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Effect of in ovo injection of cadmium on chicken embryo heart

Cited by 10 publications

References 31 publications

Protection Against Cadmium-Induced Abnormalities and Hepatotoxicity in ovo by Allium sativum

Protection Against Cadmium-Induced Abnormalities and Hepatotoxicity in ovo by Allium sativum

Cardiotoxicity of Cadmium and Its Effects on Heart Efficiency During Early and Late Chick Embryogenesis

Cadmium induced cardiac toxicology in developing Japanese quail (Coturnix japonica): Histopathological damages, oxidative stress and myocardial muscle fiber formation disorder

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