Effect of in ovo glyphosate injection on embryonic development, serum biochemistry, antioxidant status and histopathological changes in newly hatched chicks

Fathi, Mohamed; Abdelghani, Ezaldeen; Shen, Dan; Ren, Xin; Dai, Pengyuan; Li, Zhaojan; Tang, Qian; Li, Yansen; Li, Chunmei

doi:10.1111/jpn.13181

Cited by 17 publications

(19 citation statements)

References 52 publications

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“…In parallel, we observed increased lipid damage in brain tissue, yet antioxidant enzyme activities were not altered. However, it must be noted that the abovementioned experimental studies using in ovo injections, researchers applied larger GBH doses compared to those in our experiment (Fathi et al, 2019 ca 10mg/kg, >10x higher than in our study; Paganelli et al, 2010 ca 50mg/kg, i.e. >50x our study), which may explain the different responses.…”

Section: Discussioncontrasting

confidence: 62%

“…Our results support those of previous studies of poultry suggesting that maternal transfer of GBHs to offspring substantially influences offspring via the altering of cell oxidative status. Studies with chicken embryos experimentally exposed to GBHs have shown teratogenic effects on early embryonic development, lower hatchability, and altered serum chemistry (Fathi et al, 2019; Paganelli et al, 2010). Recently, decreased antioxidant enzymes activities (GP, SOD, and CAT) and increased damage to lipids, liver tissue, and kidney tissue post-hatching have been detected (Fathi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Studies with chicken embryos experimentally exposed to GBHs have shown teratogenic effects on early embryonic development, lower hatchability, and altered serum chemistry (Fathi et al, 2019; Paganelli et al, 2010). Recently, decreased antioxidant enzymes activities (GP, SOD, and CAT) and increased damage to lipids, liver tissue, and kidney tissue post-hatching have been detected (Fathi et al, 2019). In parallel, we observed increased lipid damage in brain tissue, yet antioxidant enzyme activities were not altered.…”

Section: Discussionmentioning

confidence: 99%

“…The importance of poultry in food production also calls for more attention on the effects of GBHs in birds. In the two available studies of poultry and GBH-related maternal effects, a direct injection of a relatively high concentration of Roundup (10 mg/kg glyphosate) was found to decrease hatchability, induce oxidative stress and cause damage to lipids in the exposed chicks, as compared to the control group (Fathi et al, 2019), potentially via the disruption of retinoid acid signaling (Paganelli et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Parental effects via glyphosate-based herbicides in a bird model?

Ruuskanen

Rainio

Uusitalo

et al. 2019

Preprint

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Controversial glyphosate-based herbicides (GBHs) are the most frequently used herbicides across the globe. In an increasing number of studies, researchers have identified GBH residues in soil, water, crops, and food products exposing non-target organisms to health risks; these organisms include wildlife, livestock, and humans. However, GBH-related parental effects are poorly understood. In the case of birds, GBHs may be transferred directly from mother to developing offspring (i.e. direct effects) via eggs, or they may indirectly influence offspring performance by altered maternal condition or resource allocation to eggs, for example. We experimentally exposed a parental generation of Japanese quails (Coturnix japonica) to GBHs or respective controls, recorded egg quality and glyphosate residues in eggs, and studied embryonic development and oxidative biomarkers. Glyphosate accumulated in eggs (ca 0.76 kg/mg). Embryonic development tended to be lower in eggs of GBH-exposed parents compared to control parents. Embryonic brain tissue from GBH-exposed parents tended to express more lipid damage. Given that we detected no differences in egg quality (egg, yolk, or shell mass, or egg hormone concentration) across the treatment groups, our results suggest these are likely direct effects of GBHs on offspring rather than indirect effects via altered maternal allocation of resources or hormonal signals.CapsuleExperimental, long-term parental exposure to GBHs tends to hinder offspring embryonic development and increase embryonic oxidative damage to lipids in a bird model.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Parental effects via glyphosate-based herbicides in a bird model?

Ruuskanen

Rainio

Uusitalo

et al. 2019

Preprint

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show abstract

“…The importance of poultry in food production also calls for more attention on residues and the effects of GBHs in birds. In the two available studies of poultry and GBH-related maternal effects, a direct injection of a relatively high concentration of RoundUp (10 mg/kg glyphosate) in eggs was found to decrease hatchability, induce oxidative stress and cause damage to lipids in the exposed chicks, as compared to the control group 23,24 , potentially via the disruption of retinoid acid signaling 12 .…”

mentioning

confidence: 99%

Effects of parental exposure to glyphosate-based herbicides on embryonic development and oxidative status: a long-term experiment in a bird model

Ruuskanen

Rainio

Uusitalo

et al. 2020

Sci Rep

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Controversial glyphosate-based herbicides (GBHs) are the most frequently used herbicides globally. GBH residues are detected in soil, water, crops, and food products, potentially exposing non-target organisms to health risks; these organisms include wildlife, livestock, and humans. However, the potential for GBH-related parental effects are poorly understood. In the case of birds, GBHs may be transferred directly from mothers to eggs, or they may indirectly influence offspring performance by altered maternal resource allocation to eggs. We experimentally exposed a parental generation of Japanese quails (Coturnix japonica) to GBHs (200 mg/kg feed) or respective controls. Glyphosate residues were found in eggs (ca 0.76 kg/mg). Embryonic development tended to be poorer in the eggs of GBH-exposed parents (76% of eggs showed normal development) compared to control parents (89% normal eggs). Embryonic brain tissue from GBH-exposed parents tended to express more lipid damage (20% higher), yet other biomarkers showed no apparent differences. We detected no differences in egg quality (egg, yolk, or shell mass, egg hormone concentration) across the treatment groups. Given this is the first long-term study testing parental effects of GBHs with birds, more studies are needed characterizing GBH-associated changes in maternal allocation and for example epigenetic programming.

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Investigating adverse effects of chronic dietary exposure to herbicide glyphosate on zootechnical characteristics and clinical, biochemical and immunological blood parameters in broiler chickens

Yildirim,

Laptev,

Tiurina

et al. 2023

Vet Res Commun

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Although the herbicide glyphosate is widely used globally and considered safe, more evidence of its adverse effects on animals and humans is accumulating. The present investigation was aimed at evaluating the impact of different glyphosate concentrations on zootechnical characteristics and clinical, biochemical and immunological blood parameters in Ross 308 broiler chickens. Four groups were employed, including untreated control and three experimental groups fed diets enriched with glyphosate at doses of 10, 20 and 100 ppm that conformed to 0.5, 1 and 5 maximum residue limits, respectively. The results showed that glyphosate is a stress factor triggering a multifaceted effect on important blood parameters (e.g., white blood cell and phagocytic counts), which was shown for the first time in the experiments involving productive meat-type poultry. It was first revealed that glyphosate-induced changes in blood parameters may be related to a negative impact on the zootechnical characteristics including the digestive tract organ development and body weight gain. The study findings suggested that exposure to glyphosate in the feedstuffs can adversely affect the physiological condition and productivity of broilers.

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Effect of in ovo glyphosate injection on embryonic development, serum biochemistry, antioxidant status and histopathological changes in newly hatched chicks

Cited by 17 publications

References 52 publications

Parental effects via glyphosate-based herbicides in a bird model?

Parental effects via glyphosate-based herbicides in a bird model?

Effects of parental exposure to glyphosate-based herbicides on embryonic development and oxidative status: a long-term experiment in a bird model

Investigating adverse effects of chronic dietary exposure to herbicide glyphosate on zootechnical characteristics and clinical, biochemical and immunological blood parameters in broiler chickens

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