Effect of in ovo feeding of probiotic, prebiotic and synbiotic to Broiler embryos on growth performance, Mucin-2 gene expression and gut colonization of microbiota

Abstract

Supplementation of antibiotics in poultry diet was banned in several countries due to development of antibiotic resistance. In ovo feeding of prebiotics, probiotics and synbiotics have gained more attention recently. The present study was carried out in 2018 to investigate the effect of in-ovo feeding of probiotic, prebiotic and symbiotic on growth performance and gut microbiome of broiler chicken. On 18th day of incubation, 600 eggs were randomly divided into five treatments each with four replicates of 30 eggs each and were injected with different bio-active compounds, viz. 0.2 ml of Lactobacillus acidophilus 3×107 cfu, 0.5% Mannan-oligosaccharide (MOS), synbiotic (0.1 ml each of Lactobacillus acidophilus 3×107 cfu and 0.5% MOS) along with injected and non-injected controls. After hatch, 400 chicks were sorted out as per treatment with four replicates of 20 chicks each. Birds were reared under deep litter system and fed with experimental diet ad lib. In ovo feeding of Lactobacillus acidophilus, MOS either separately or in combination significantly improved hatch weight, fifth week body weight and gain. However, hatchability, cumulative feed intake, cumulative FCR and cumulative livability were not affected. Improved colonization of Lactobacillus acidophilus and suppressed colonization of Salmonella, Escherichia coli and Staphylococcus in all intra-amniotic groups was noticed. Ileal Mucin-2 gene was significantly up-regulated in the order of MOS, L. acidophilus and synbiotic injected broilers. The results concluded that the in ovo delivery of Lactobacillus acidophilus and MOS either separately or in combination had beneficial effect on growth and gut health of broiler chicken.

Effect of different synbiotic administration methods on growth, carcass characteristics, ileum histomorphometry, and blood biochemistry of Cobb-500 broilers

“…In ovo feeding with Lactobacillus acidophilus and mannan-oligosaccharide, or their combination, significantly enhanced hatch weight without influencing hatchability or livability [ 36 ]. Hatchability was negatively influenced by in ovo synbiotic [ 37 ], which supports the present study findings.…”

Probiotics and vitamins modulate the cecal microbiota of laying hens submitted to induced molting

“…Reduced levels of Bacteroides and Alistipes and increased levels of Lactobacillus are all signs that resveratrol has helped reestablish a balance to the gut microbiota ( Ding et al, 2021 ). Particularly, prebiotics like mannan oligosaccharide will also promote L. acidophilus colonization and inhibit the colonization of SE , E. coli and Staphylococcus aureus with therapeutic doses ( Swapnil et al, 2021 ).…”