Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C

Komarevtsev, S. K.; Timorshina, Svetlana; Leontieva, Maria R.; Shabunin, S. V.; Лобакова, Е. С.; Osmolovskiy, A. A.

doi:10.1134/s0003683821030078

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…The immobilised cells of Aspergillus ochraceus VKM-F4104D mycelium demonstrated increased stability and were able to synthesise fibrinolytic protease at the maximum level for 10 or more days. In addition, it was shown that immobilised mycelial cells of the producer during cultivation can be used repeatedly (up to five cycles) [147].…”

Section: Streptomyces Radiopugnans_vitsd8mentioning

confidence: 99%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis. Numerous methodologies including the use of anti-coagulants, anti-platelet drugs, surgical operations and fibrinolytic enzymes are employed for the dissolution of fibrin clots and hence ameliorate thrombosis. Microbial fibrinolytic enzymes have attracted much more attention in the management of cardiovascular disorders than typical anti-thrombotic strategies because of the undesirable after-effects and high expense of the latter. Fibrinolytic enzymes such as plasminogen activators and plasmin-like proteins hydrolyse thrombi with high efficacy with no significant after-effects and can be cost effectively produced on a large scale with a short generation time. However, the hunt for novel fibrinolytic enzymes necessitates complex purification stages, physiochemical and structural-functional attributes, which provide an insight into their mechanism of action. Besides, strain improvement and molecular technologies such as cloning, overexpression and the construction of genetically modified strains for the enhanced production of fibrinolytic enzymes significantly improve their thrombolytic potential. In addition, the unconventional applicability of some fibrinolytic enzymes paves their way for protein hydrolysis in addition to fibrin/thrombi, blood pressure regulation, anti-microbials, detergent additives for blood stain removal, preventing dental caries, anti-inflammatory and mucolytic expectorant agents. Therefore, this review article encompasses the production, biochemical/structure-function properties, thrombolytic potential and other surplus applications of microbial fibrinolytic enzymes.

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Section: Streptomyces Radiopugnans_vitsd8mentioning

confidence: 99%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

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Section: Introductionmentioning

confidence: 99%

“…On the other hand, the adaption of Aspergillus to shifting environments lead to the formation of a particular set of proteins ( Day and Quinn, 2019 ; Wang et al, 2022b ). A. ochraceus has been an important source of industrial enzymes like protease ( El-Khonezy et al, 2021 ; Komarevtsev et al, 2021 ; Zhu et al, 2021 ), esterase ( Romero-Borbón et al, 2018 ) and tannase ( Aracri et al, 2019 ). Several medicinal metabolites, such as an intermediate for the synthesis of desogestrel and eplerenone, are characterized from A. ochraceus ( Wang X. et al, 2020 ; Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The Secondary Metabolites and Biosynthetic Diversity From Aspergillus ochraceus

Chen

et al. 2022

Front. Chem.

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Aspergillus ochraceus, generally known as a food spoilage fungus, is the representative species in Aspergillus section Circumdati. A. ochraceus strains are widely distributed in nature, and usually isolated from cereal, coffee, fruit, and beverage. Increasing cases suggest A. ochraceus acts as human and animal pathogens due to producing the mycotoxins. However, in terms of benefits to mankind, A. ochraceus is the potential source of industrial enzymes, and has excellent capability to produce diverse structural products, including polyketides, nonribosomal peptides, diketopiperazine alkaloids, benzodiazepine alkaloids, pyrazines, bis-indolyl benzenoids, nitrobenzoyl sesquiterpenoids, and steroids. This review outlines recent discovery, chemical structure, biosynthetic pathway, and bio-activity of the natural compounds from A. ochraceus.

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Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C

Cited by 2 publications

References 19 publications

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

The Secondary Metabolites and Biosynthetic Diversity From Aspergillus ochraceus

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