Effect of IL-4 on peripheral blood lymphocyte proliferation: implication in immunopathogenesis of H. pylori infection

Fan, Xue‐Gong; Yakoob, Javed; Fan, X. G.; Keeling, P. W. N.

doi:10.1016/0165-2478(95)02440-9

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…IL-4 inhibits the inflammatory action in pathological conditions such as H. pylori , which triggers its plasma and gastric levels [ 29 , 47 ]. Conversely, some studies advocated that IL-4 has no effect on the prompted blood lymphocytes during H. pylori infection [ 30 , 31 ]. In the present study, we reported that immunization of the BALB/c mice with H. pylori -related peptides augments the IL-4 production after H. pylori infection challenge.…”

Section: Discussionmentioning

confidence: 99%

“…Another study observed that human gastric epithelium cells produce IL-4 in response to H. pylori infection [ 30 ]. On the other hand, the increased IL-4 production in the study conducted by Fan et al did not suppress proliferation of peripheral blood lymphocytes in H. pylori infection [ 31 ]. Another study reported that IL-4 suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α and IL-1β, by the gastric mononuclear cells in patients with H. pylori infection [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice

AlEraky

Abuohashish

Bugshan

et al. 2022

IJMS

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Helicobacter pylori (H. pylori) has been identified as a group-1 definite carcinogen. As of yet, there is no available vaccine for this microorganism. Our study aimed to identify antigenic peptides in H. pylori using an in silico proteomic approach, and to evaluate their effectiveness as potential vaccine candidates. Four different peptide sequences were prioritized using the reverse vaccinology, namely, CagA1, CagA2, VacA, and SabA. Peptides emulsified with Freunde’s adjuvant were used to immunize BALB/C mice. Subcutaneously immunized mice were challenged by oral administration of H. pylori. IgG, IgA, IL4, and IL17 were detected in mice sera. Histopathology of the dissected stomach of vaccinated and control mice were assessed using H&E stain. IgG was significantly higher in mice vaccinated with SabA. IL-4 was significantly increased in CagA1, CagA2, VacA, and SabA vaccinated mice compared to the adjuvant group. Additionally, histopathological examination of gastric tissue showed a protective effect in the vaccinated groups compared to adjuvant and PBS groups. Our findings indicate a promising effect of the tested epitopes, particularly the SabA antigen, to induce an immune response against H. pylori.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice

AlEraky

Abuohashish

Bugshan

et al. 2022

IJMS

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Telomerase activity of peripheral blood lymphocytes in patients with chronic hepatitis B

Fan¹,

Huang²,

Tang³

et al. 2000

Immunology Letters

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Effect of IL‐12 on T‐cell immune responses in patients with chronic HCV infection

Fan

Tang²,

Yi³

et al. 2000

APMIS

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As the host's immune responses may determine the outcome of hepatitis C virus (HCV) infection, and interleukin (IL)‐12 plays an essential role in host defense against infectious diseases, we studied the antigen‐specific and non‐specific cellular immune responses in patients with chronic HCV infection. A proliferative response to phytohemagglutinin (PHA) was found in all 20 patients. Of the 20, 8 (40%) displayed a lymphocyte proliferation in response to HCV antigen c22, 2 (10%) to c33, 6 (30%) to c100–3, and 1 (5%) to NS5. The addition of rhIL‐12 to cultures of peripheral blood mononuclear cells (PBMC) stimulated with PHA significantly enhanced the proliferative responses in normal controls as well as in HCV‐infected subjects. The increased proliferation was also observed in HCV‐infected patients when PBMC were co‐cultured with HCV antigens c22 and c 100–3 in the presence of rhIL‐12. The production of interferon γ (IFNγ), IL‐2, IL‐4 and IL‐10 was observed in 7 (58.3%), 5 (41.7%,), 3 (25.0%) and 5 (41.7%) HCV‐infected individuals stimulated with c22, and in 4 (33.3%), 2 (16.7%), 2 (16.7%) and 2 (16.7%) with c100–3, respectively. All HCV‐infected individuals had increased production of cytokines IFNγ, IL‐2, IL‐4 and IL‐10 in supernatants of PBMC after stimulation with PHA. IL‐12 significantly augmented Th1 cytokine production in HCV‐infected individuals stimulated with PHA and with HCV antigens. In conclusion, deficient cellular immune responses are present in HCV‐infected patients and IL‐12 can enhance the immune responses in these patients.

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Effect of IL-4 on peripheral blood lymphocyte proliferation: implication in immunopathogenesis of H. pylori infection

Cited by 5 publications

References 16 publications

Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice

Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Helicobacter pylori Infection in BALB/c Mice

Telomerase activity of peripheral blood lymphocytes in patients with chronic hepatitis B

Effect of IL‐12 on T‐cell immune responses in patients with chronic HCV infection

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