Effect of <i>trans‐10 cis‐12</i> conjugated linoleic acid on Bovine Oocyte Competence and Fatty Acid Composition

Lapa, M; Marques, C.C.; Alves, Susana P.; Vasques, M.I.; Baptista, M.C.; Carvalhais, I.; Pereira, Martinha; Horta, A.E.M.; Bessa, Rui J. B.; Pereira, Rúben

doi:10.1111/j.1439-0531.2011.01762.x

Cited by 72 publications

(71 citation statements)

References 30 publications

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“…Reports confirm the reduction of lipid accumulation in in vitro embryos (Pereira et al, 2007 and or the decrease in lipogenesis of porcine adipose explants (José et al, 2008) after culture with this CLA isomer. Moreover, the presence of t10,c12 CLA during maturation improved bovine oocyte developmental competence (Lapa et al, 2011). Although these latter results at present have not been confirmed for porcine oocytes, t10,c12 CLA during maturation clearly interfered with LD organization and probably with their composition reflected by a lighter colour tone of their cytoplasm.…”

Section: Discussionmentioning

confidence: 62%

“…Indeed, in vitro-produced bovine embryos cultured with 100 mM t10,c12 CLA were characterized by a reduced lipid accumulation and an improved blastocyst cryosurvival (Pereira et al, 2007 and. Moreover, alterations in the lipid profile of bovine oocytes matured with this CLA isomer were associated with an enhanced blastocyst quality (Lapa et al, 2011). In pig, t10,c12 CLA exerts specific effects on adipocytes by diminishing fat deposition through lipogenesis reduction, lipolysis and fatty acid oxidation increase (Corino et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Prates

Marques

Baptista

et al. 2013

Animal

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Lipid droplets (LD) in porcine oocytes form a dark mass reaching almost all cytoplasm. Herein we investigated changes in fat areas, cytoplasmic tone and LD morphology duringin vitromaturation (IVM) of porcine oocytes cultured with 100 μMtrans-10,cis-12 conjugated linoleic acid (t10,c12 CLA) or 10 μM forskolin at different time periods. Four groups were constituted: control, excipient,t10,c12 CLA and forskolin, with drugs being supplemented during 44 to 48 h and the initial 22 to 24 h in Experiments 1 and 2, respectively. In Experiment 3, forskolin was supplemented for the first 2 h. Matured oocytes were inseminated with frozen-thawed boar semen and cleavage rate recorded. Before and during IVM, samples of oocytes were evaluated for LD, total and fat areas and fat gray value or for meiotic progression. Results showed that forskolin supplementation during 44 to 48 h or 22 to 24 h inhibits oocyte maturation (exp. 1: forskolin = 5.1 ± 8.0%, control = 72.6 ± 5.0%; exp. 2: forskolin = 24.3 ± 7.4%, control = 71.6 ± 5.6%) and cleavage (exp. 1: forskolin = 0.0 ± 0.0%, control = 55.4 ± 4.1%; exp. 2: forskolin = 8.3 ± 3.3%, control = 54.5 ± 3.0%). Forskolin also reduced oocyte and fat areas. In Experiment 3, forskolin negative effect on oocyte maturation and cleavage disappeared, although minor (P⩽ 0.03) LD and oocyte fat areas were identified at 22 to 24 h of IVM. Oocytes supplemented witht10,c12 CLA during 44 to 48 h presented a lighter (P⩽ 0.04) colour tone cytoplasm than those of control and forskolin. In conclusion,t10,c12 CLA and forskolin were capable of modifying the distribution and morphology of cytoplasmic LD during porcine oocyte maturation, thus reducing its lipid content in a time-dependent manner.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Prates

Marques

Baptista

et al. 2013

Animal

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“…Although it has been previously shown that the supplementation of maturation medium with linoleic acid improves blastocyst quality (23), it was also indicated that such supplementation reduces the proportion of oocytes developing to the metaphase 2 stage and thus the rate of embryos developing to the blastocyst stage. This adverse effect of linoleic acid is attributed to it increasing the concentration of prostaglandin E2 and the level of intracellular cAMP and reducing the phosphorylation of mitogen-activated protein kinases in the medium (24).…”

Section: Discussionmentioning

confidence: 99%

The effect of oleic and linoleic acids on in vitro bovineembryonic development and embryo quality

Karaşahi̇n¹,

Arıkan²

2015

Turk J Vet Anim Sci

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IntroductionIt has been proven that the morphological and physiological quality of in vivo-derived embryos is better than that of in vitro-derived embryos (1,2). One of the major reasons for the relative inadequacy of in vitro embryonic development is the generation of reactive oxygen species (ROS) (2). ROS generation occurs as a result of the reduction of free oxygen during the metabolism of molecular oxygen required for embryonic development. The major ROS produced by the embryo itself are the superoxide anion radical, hydrogen peroxide, and the hydroxyl radical. When released at a basal level, ROS have a regulatory effect on embryonic metabolism, embryonic development, and embryo implantation (3).Oxidative stress-induced damage to the embryo arises from excessively released ROS, and these radicals pass the cell membrane, causing structural changes in cellular molecules such as lipids, proteins, and nucleic acids, which, in turn, result in mitochondrial alterations, blockage of cellular development in the embryo, excessive consumption of ATP, and apoptosis (3-5). Eventually, embryonic development is inhibited and the implantation of the embryo is negatively affected (3).Against the oxidative stress and damages caused by ROS, in vivo antioxidant defense systems, which protect the embryo, exist in both the embryo and the female reproductive tract that it resides in. These antioxidant systems include, among others, glutathione, taurine, hypotaurine, ascorbate, pyruvate, vitamin E, glutathione peroxidase, superoxide dismutase, and catalase (3). Under in vitro conditions, as the embryo depends only on the antioxidant systems it is itself equipped with against oxidative stress, additional preventive measures need to be taken (6-8). However, the elimination of oxidative stress and oxidative stress factors from the embryo culture medium is both an inevitable and complex problem. The selection of antioxidants and antioxidant doses for the supplementation of culture media can be challenging, as excessive antioxidant doses in the medium may cause negative impacts (6,9).Previous research has shown that fatty acids incorporated into in vitro culture media exhibit positive effects on oocyte maturation, fertilization, and embryonic development (10,11). The feeding of ruminants on rations of high fat content produces positive impacts on reproductive performance and enables high blastocyst

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“…Most information regarding lipid structural analysis from oocytes and embryos has been obtained by staining methods, such as Nile Red, which quantify cytoplasmic lipids in general and gas chromatography (GC) that quantify by fatty acid (FA) residues extracted from the total sample lipid content (BARCELÓ-FIMBRES;SEIDEL JR., 2011;KIM et al, 2001;LAPA et al, 2011;MATORRAS et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

Gonçalves

Ferreira

Orlandi

et al. 2014

Braz. J. Vet. Res. Anim. Sci.

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It was reported the potential of MALDI-MS for the characterization of lipid species present in a single equine embryo, and studied some lipid structures detected by collision induced dissociation (CID) experiments. In the positive ion mode spectrum, it were observed mostly protonated and sodiated species of sphingomyelins (SM), phosphatidylcholines (PC) and triacylglycerols (TAG). In the negative ion mode, it were observed phosphatidylethanolamines (PE) and phosphatidylinositols (PI). MS/MS spectrum of most intense lipid ions was performed to show MALDI-MS/MS structural information potential.

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Effect of trans‐10 cis‐12 conjugated linoleic acid on Bovine Oocyte Competence and Fatty Acid Composition

Cited by 72 publications

References 30 publications

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

The effect of oleic and linoleic acids on in vitro bovineembryonic development and embryo quality

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

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