Effect of <i>Tagetes minuta</i> oil on larval morphology of <i>Plutella xylostella</i> through scanning electron microscopy and mechanism of action by enzyme assay

Dolma, Shudh Kirti; Jayaram, Ch.; Chauhan, Nandita; Reddy, S. G. Eswara

doi:10.1080/15569543.2021.1988980

Cited by 8 publications

(9 citation statements)

References 36 publications

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“…The AChE activities in A. craccivora fed with bean leaf discs treated with different concentrations of LEE, BEE, and SO showed significant inhibition compared to control aphids. Present results were confirmed with the findings of Dolma et al [ 52 ], who reported that Tagetes minuta oil showed an inhibitory effect on AChE in P. xylostella . In a similar study, essential oils and synthesized nano-emulsions from Basilicum ocimum L., Cuminum cyminum L., Origanum marjorana L., and Matricaria chamomilla L. showed decreased activity of AChE in A. craccivora [ 53 ].…”

Section: Discussionsupporting

confidence: 93%

“…Results conform with the findings of Phankaen et al [ 54 ], who reported that caffeine from Coffea arabica extract inhibits GST and carboxylesterase (CarE) in Tribolium castaneum . Similarly, a previous study also showed that Tagetes minuta oil inhibited GST in P. xylostella [ 52 ].…”

Section: Discussionmentioning

confidence: 60%

“…Four different concentrations of LEE, BEE, and SO (0.25%, 0.5%, 1%, and 2%) were chosen for enzyme activity [ 52 ] according to the residual toxicity assay described above. The nymphs of A. craccivora of each concentration after 48 h of treatment were homogenized and centrifuged at 15,000 rpm at 4 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

2022

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Aphid, Aphis craccivora Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss. Indiscriminate and repeated use of insecticides for control of aphid leads to the development of resistance, and is harmful to the environment, non-target organisms, etc. Plant-based extracts/seed oils (SO) are the best alternatives to insecticides. Insecticidal activities of Triadica sebifera have not been reported against A. craccivora and other insect pests to date. In the current study, the main objective was to study the insecticidal activities of leaf/bark extracts/fractions, seed oil, isolated compounds, and their combinations against A. craccivora. Results showed that, among the extracts, ethanolic bark extract 80% (LC50 = 5115.98 mg/L) was more effective against A. craccivora. Among fractions, the n-hexane fraction of leaves (LC50 = 425.73 mg/L) and the ethyl acetate fraction of bark (LC50 = 813.45 mg/L) were promising. Among compounds, gallic acid was the most effective (LC50 = 1303.68 mg/L) compared to shikimic acid and quercetin. SO (LC50 = 850.94 mg/L) was superior compared to extracts/fractions/compounds. All the combinations showed toxicity and synergistic activity. Leaf/bark extracts and SO significantly inhibited the AChE and GST activity in A. craccivora. Based on field bio-efficacy, the leaf extract/SO or their combinations can be recommended for the control of aphids.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 60%

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Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

2022

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“…Subsequently, GST catalyzes the synthetic pesticides usually later in the phase I metabolic process (Kranthi, 2005). The current findings were supported by the results of Dolma et al (2021), stating that T. minuta oil inhibited the AChE and GST activity against P. xylostella. Similarly, the essential oil from Artemisia maritima inhibited the GST activity against C. chinensis and C. maculatus, as reported previously (Chauhan et al, 2022).…”

Section: Discussionsupporting

confidence: 84%

Toxicity and synergistic activity of compounds from essential oils and their effect on detoxification enzymes against Planococcus lilacinus

2022

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Mealybug, Planococcus lilacinus Cockerell, is a primary surface-feeding insect pest of fruit and flowering plants and also transmits plant viruses, resulting in economic crop loss. Inappropriate and recurrent use of pesticides for mealybug control results in resistance building and deleterious effects on humans and the environment. Essential oils are the most excellent choice for insecticides. Insecticidal activities of pure compounds of essential oils against P. lilacinus are not reported. The present study aims to study the insecticidal activities of some pure active compounds and their binary mixture’s action by topical application against P. lilacinus. Results showed that the pure compounds of L-limonene, β-myrcene, and ocimene revealed toxicity (each at LD50 = 0.37 µg/insect) after 96 h. The binary mixtures of geraniol + L-menthol and L-limonene + geraniol exhibited synergistic effects (each at LD50 = 0.03 µg/insect) after 96 h. The monoterpenes of ocimene and β-myrcene at the higher concentration of 5,000 ppm substantially inhibited the detoxification enzyme activities of AChE (0.93 and 0.78 mU/mg, respectively) and GST (2.19 and 7.29 nmol/min/ml, respectively) in P. lilacinus after 48 h. SEM analysis reported the significant anomalies on the morphology of abdominal cuticle, setae, and thoracic leg after 96-h treatment with ocimene at 1,250 ppm against P. lilacinus. Based on the results, the tested pure compounds and their combinations can be suggested for the control of mealybugs.

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“…Present results con rmed with the ndings of Dolma et al 32 who reported Tagetes minuta oil showed inhibitory effect on AChE in P. xylostella. In a similar study, essential oils and synthesized nano-emulsion from Basilicumocimum L., Cuminum cyminum L., Origanum marjorana L. and Matricaria chamomilla L. showed decreased activity of AChE in A. craccivora 33 . Similarly for the GST assay, all the concentrations of LEE, BEE and SO signi cantly inhibit the GST activity as compared to control.…”

Section: Discussionmentioning

confidence: 75%

Insecticidal and Enzyme Inhibition Activities of Leaf/bark Extracts, Fractions, Seed Oil and Isolated Compounds From Triadica Sebifera (L.) Small Against Aphis Craccivora Koch

Dolma

Singh

Reddy

2021

Preprint

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Aphid, Aphis craccivora Koch (Hemiptera: Aphididae) is a major sap-sucking insect pest of leguminous crops and also transmit plant viruses leading to economic yield loss. Insecticidal activities of T. sebifera have not been reported against A. craccivora and other insect pests till date. In this study, residual toxicity of leaf/bark extracts, seed oil, fractions, isolated compounds and its combinations; synergistic activity of leaf/ bark extract with seed oil and its enzyme inhibition activities against A. craccivora was studied. Among extracts, ethanolic bark extract 80% (LC50=5115.98 mg L-1) was more effective against A. craccivora than other extracts. Among fractions, n-hexane fraction of leaf (LC50=425.73 mg L-1) and ethyl acetate fraction of bark (LC50=813.45mg L-1) was more effective. Among compounds, gallic acid was most effective (LC50=1303.68 mg L-1) than shikimic acid and quercetin (LC50=1725.09 and 1855.93 mg L-1, respectively). Seed oil (LC50=850.94 mg L-1) is more superior than extracts/fractions/compounds. All the combinations showed toxicity and synergistic activity. Leaf/bark extracts and seed oil (0.25-2 mg L-1) significantly inhibits the AChE and GST activity in A. craccivora. Based on field bio-efficacy studies, the leaf extract/seed oil or their combinations can be recommended for development of botanical formulation for the control of aphid.

show abstract

Effect of Tagetes minuta oil on larval morphology of Plutella xylostella through scanning electron microscopy and mechanism of action by enzyme assay

Cited by 8 publications

References 36 publications

Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

Toxicity and synergistic activity of compounds from essential oils and their effect on detoxification enzymes against Planococcus lilacinus

Insecticidal and Enzyme Inhibition Activities of Leaf/bark Extracts, Fractions, Seed Oil and Isolated Compounds From Triadica Sebifera (L.) Small Against Aphis Craccivora Koch

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