Effect of<i>Salvia mirzayanii</i>on the immune system and induction of apoptosis in peripheral blood lymphocytes

Amirghofran, Zahra; Bahmani, Maryam; Azadmehr, Abbas; Javidnia, Katayoun; Ramazani, Mohammad; Ziaei, Akram

doi:10.1080/14786410802267502

Cited by 12 publications

(6 citation statements)

References 17 publications

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“…The induction of apoptosis by the extract was additionally proven by the typical DNA laddering in internucleosomal DNA fragmentation analysis of mitogen-stimulated human lymphocytes. 8 Salvia mirzayanii methanol extract was also injected to mice to assay the effects on the humoral immunity as measured by thickness of the foot pad after challenge. Outcomes revealed improvement of the response at both primary and secondary exposure to the antigen after administration of lower concentrations of extract.…”

Section: Immunomodulatory Effectsmentioning

confidence: 99%

“…After a dose of 100 mg/kg, the effect was decreased. 8 Because of the fact that teuclatriol and spathulenol, isolated in an activity-guided approach from the extract, showed considerable antiproliferative effects on human peripheral lymphocytes with IC 50 values of 74.2 + 5.4 and 85.4 + 11.08 mg/mL, respectively, the immunomodulation by SM was attributed to these compounds. 19,21…”

Section: Immunomodulatory Effectsmentioning

confidence: 99%

“…7 Folk practitioners and local healers usually have reported the effectiveness of SM in diabetes, spasms, gastrointestinal disorders, infections, and inflammations. 8,9 In addition to these applications, SM is still used as an astringent and tonic in Iranian folk medicine.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical and Pharmacological Aspects of Salvia mirzayanii Rech. f. & Esfand

Zarshenas

Krenn

2014

J Evid Based Complementary Altern Med

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Salvia mirzayanii Rech. f. & Esfand is an endemic herbaceous plant belonging to the Lamiaceae family. The plant grows in the center and south of Iran and is broadly used in folk medicine. This review focuses on phytochemical and pharmacological data of S mirzayanii. Databases such as PubMed, Scopus, Web of Science, Scientific Information Database (Iranian database involving English and Persian articles), and Google Scholar were searched for terms "Salvia mirzayanii," "Iranian sage," "Marv-e-Talkh," and "Moor-e-Talkh" in both Persian and English up to December 10, 2013. In line with the ethnopharmacological uses, antioxidant, immunomodulatory, anti-inflammatory, antimicrobial, and insecticidal activities of different preparations of S mirzayanii have been shown in recent studies. The antimicrobial activity against human pathogenic strains has mainly been attributed to the essential oil of S mirzayanii, which in numerous analyses has been investigated either in plants of different origin or prepared by differing extraction methods. Despite the studies on antioxidant or antimicrobial activities, profound research on the toxicity or clinical properties of the herb is missing.

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Section: Immunomodulatory Effectsmentioning

confidence: 99%

Section: Immunomodulatory Effectsmentioning

confidence: 99%

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Phytochemical and Pharmacological Aspects of Salvia mirzayanii Rech. f. & Esfand

Zarshenas

Krenn

2014

J Evid Based Complementary Altern Med

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“…mirzayanii (Marv-e-Talkh in Persian) and S. macrosiphon (Marvak in Persian) are two well-known endemic species of the Salvia genus in Iran. S. mirzayanii has been used in Iranian folk medicine especially in Hormozgan and Kerman provinces for the treatment of diabetes, spasms, gastrointestinal disorders, infections, and inflammations (18). Local healers in Iran still use S. mirzayanii as a tonic and astringent.…”

Section: Introductionmentioning

confidence: 99%

Antidepressant Effects of the Aqueous and Hydroalcoholic Extracts of Salvia mirzayanii and Salvia macrosiphon in Male Mice

et al. 2019

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Objectives: As some species of the Salvia family have been shown to exert antidepressant-like activity, the aim of this study was to investigate the antidepressant effects of the aqueous and hydroalcoholic extracts of Salvia mirzayanii and Salvia macrosiphon, two endemic species of salvia in Iran. Methods: In total, 148 eight-week-old male albino mice (25-35 g) were used to carry out the forced swimming test. The effects of different doses of the aqueous (100-1800 mg/kg) and hydroalcoholic extracts (75-900 mg/kg) of Salvia mirzayanii and Salvia macrosiphon on immobility, climbing, and swimming behaviors were examined. Fluoxetine and imipramine were used as control drugs. The effects of extracts on locomotor activity were also evaluated. Results: High doses of the aqueous extracts of both plants and hydroalcoholic extract of Salvia mirzayanii produced a significant reduction in immobility and increase in swimming compared to the control group. The hydroalcoholic extract of Salvia macrosiphon did not show any significant effect on immobility and swimming. Only some doses of hydroalcoholic extract of Salvia mirzayanii and aqueous extract of Salvia macrosiphon showed a significant increase in climbing behavior. The aqueous and hydroalcoholic extract of both plants caused a decrease in spontaneous activity. Conclusions: The findings of this study indicated that the aqueous and hydroalcoholic extracts of Salvia mirzayanii and the aqueous extract of Salvia macrosiphon had antidepressant-like activity. In addition, the hydroalcoholic extract of Salvia mirzayanii and the aqueous extracts of Salvia macrosiphon were more effective fractions of these plants. These endemic Iranian Salvia species may have potential therapeutic effects for depression.

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“…Various concentrations of the extract of this herb showed an inhibitory effect on the proliferation of lymphocytes. This inhibitory effect was due mainly to the ability of the extract to induce apoptosis in activated lymphocytes (Amirghofran et al ., 2010). In the present study, bioassay guided fractionation of Salvia mirzayanii was performed to identify the active compound/s for this effect.…”

Section: Introductionmentioning

confidence: 99%

Identification of spathulenol in Salvia mirzayanii and the immunomodulatory effects

Ziaei

Ramezani

Wright

et al. 2010

Phytotherapy Research

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The methanol extract of Salvia mirzayanii has shown an immunomodulatory effect on peripheral blood lymphocytes. Bioassay-guided fractionation using a lymphocyte proliferation assay on Salvia mirzayanii was performed in order to purify and identify the active compounds. Fractionation of the methanol extract and purification of the components using normal column chromatography and preparative thin layer chromatography resulted in identification of the bioactive compound, spathulenol, with an immunoinhibitory effect. Identification of this compound was performed by 1D and 2D NMR methods and HRMS. Treatment of activated lymphocytes with a concentrated fraction containing 62% of spathulenol (SP) showed a decrease in the proliferation of lymphocytes with an IC(50) of 85.4 ± 11.08 µg/mL. Flow cytometry analysis using annexin V and propidium iodide staining of the stimulated peripheral blood lymphocytes in the presence of SP demonstrated a dose dependent increase in the percentage of apoptotic cells (IC(50) ; 77.2 ± 5.31 µg/mL). No significant increase in caspase 3 activity in a 20 h treatment of stimulated lymphocytes compared with the control was observed. In conclusion, this study identified the possible activity of spathulenol as one of the immunomodulatory compounds present in Salvia mirzayanii. SP showed the capacity to inhibit proliferation in the lymphocytes and to induce apoptosis in these cells possibly through a caspase-3 independent pathway.

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Effect ofSalvia mirzayaniion the immune system and induction of apoptosis in peripheral blood lymphocytes

Cited by 12 publications

References 17 publications

Phytochemical and Pharmacological Aspects of Salvia mirzayanii Rech. f. & Esfand

Phytochemical and Pharmacological Aspects of Salvia mirzayanii Rech. f. & Esfand

Antidepressant Effects of the Aqueous and Hydroalcoholic Extracts of Salvia mirzayanii and Salvia macrosiphon in Male Mice

Identification of spathulenol in Salvia mirzayanii and the immunomodulatory effects

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