Effect of <i>PPARGC1A</i> on the development and metabolism of early rabbit embryos in vitro

Zhang, Guomin; Guo, Yanliang; Deng, Mingtian; Wan, Yongjie; Deng, Kaiping; Xiao, Shen‐Hua; Meng, Fanjuan; Wang, Zhen; Lei, Zhihai

doi:10.1002/mrd.23269

Cited by 7 publications

(3 citation statements)

References 53 publications

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“…Among them, novel_circ_004282 and novel_circ_002121 were speculated to play important roles in regulating oxidative myofibers by PPP3CA and NFATC1 expression [ 52 ]. As a transcriptional coactivator, PGC1α is a downstream effector of AMPK signaling, has been found to regulate mitochondria biogenesis and the transformation of myofiber type [ 53 – 55 ]. In the current study, we found that circPTPN4 decreases mtDNA content and suppresses mitochondria functions.…”

Section: Discussionmentioning

confidence: 99%

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

Cai

Zhou

et al. 2022

J Animal Sci Biotechnol

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Background Circular RNAs (circRNAs) are a novel class of endogenous ncRNA, which widely exist in the transcriptomes of different species and tissues. Recent studies indicate important roles for circRNAs in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the specific role of circRNAs in myogenesis is still poorly understood. In this study, we attempted to systematically identify the circRNAs involved in myogenesis and analyze the biological functions of circRNAs in chicken skeletal muscle development. Results In total, 532 circRNAs were identified as being differentially expressed between pectoralis major (PEM) and soleus (SOL) in 7-week-old Xinghua chicken. Among them, a novel circRNA (novel_circ_002621), generated by PTPN4 gene, was named circPTPN4 and identified. circPTPN4 is highly expressed in skeletal muscle, and its expression level is upregulated during myoblast differentiation. circPTPN4 facilitates the proliferation and differentiation of myoblast. Moreover, circPTPN4 suppresses mitochondria biogenesis and activates fast-twitch muscle phenotype. Mechanistically, circPTPN4 can function as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus participating in AMPK signaling. Conclusions circPTPN4 functions as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus promoting the proliferation and differentiation of myoblast, as well as activating fast-twitch muscle phenotype.

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Section: Discussionmentioning

confidence: 99%

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

Cai

Zhou

et al. 2022

J Animal Sci Biotechnol

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“…PPARGC1A could increase the expression of transcription factor A mitochondrial ( TFAM ) to regulate mtDNA transcription and replication by stimulating a series of nuclear transcription factors, such as nuclear respiratory factor 1 ( NRF1 ) and nuclear respiratory factor 2 ( NRF2 ) [ 30 , 31 , 32 ]. Moreover, as a transcriptional coactivator, PPARGC1A has been found to be widely involved in a series of biological processes by regulating mitochondrial biogenesis and energy metabolism [ 3 , 33 , 34 , 35 ]. In this study, we found PPARGC1A increased mtDNA content and mitochondrial membrane potential to facilitate mitochondrial biogenesis.…”

Section: Discussionmentioning

confidence: 99%

PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p

Cai

Kong

et al. 2022

IJMS

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Meat production performance is one of the most important factors in determining the economic value of poultry. Myofiber is the basic unit of skeletal muscle, and its physical and chemical properties determine the meat quality of livestock and poultry to a certain extent. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) as a transcriptional coactivator has been found to be widely involved in a series of biological processes. However, PPARGC1A is still poorly understood in chickens. In this manuscript, we reported that PPARGC1A was highly expressed in slow-twitch myofibers. PPARGC1A facilitated mitochondrial biogenesis and regulated skeletal muscle metabolism by mediating the flux of glycolysis and the TCA cycle. Gain- and loss-of-function analyses revealed that PPARGC1A promoted intramuscular fatty acid oxidation, drove the transformation of fast-twitch to slow-twitch myofibers, and increased chicken skeletal muscle mass. Mechanistically, the expression level of PPARGC1A is regulated by miR-193b-3p. Our findings help to understand the genetic regulation of skeletal muscle development and provide a molecular basis for further research on the antagonism of skeletal muscle development and fat deposition in chickens.

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“…Mitochondrial biogenesis is regulated at the transcriptional, translational, and post-translational levels [17], and peroxisome proliferator-activated receptor γ co-activator 1α (PGC1α) is a key regulator of mitochondrial biogenesis [18]. In addition, NRF2, NRF1, and downstream TFAM are the main regulators of mitochondrial biosynthesis [19,20].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos

Kageyama

Ito

Shirasuna

et al. 2021

Journal of Reproduction and Development

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The number of mitochondria in blastocysts is a potential marker of embryo quality. However, the molecular mechanisms governing the mitochondrial number in embryos are unclear. This study was conducted to investigate the effect of reduced mitochondrial reactive oxygen species (ROS) levels on mitochondrial biogenesis in porcine embryos. Oocytes were collected from gilt ovaries and activated to generate over 4 cell-stage embryos at day 2 after activation. These embryos were cultured in media containing either 0.1 µM MitoTEMPOL (MitoT), 0.5 µM Mitoquinol (MitoQ), or vehicle (ethanol) for 5 days to determine the rate of development to the blastocyst stage. The mitochondrial number in blastocysts was evaluated by real-time polymerase chain reaction (PCR). Five days after activation, the embryos (early morula stage) were subjected to immunostaining to determine the expression levels of NRF2 in the nucleus. In addition, the expression levels of PGC1α and TFAM in the embryos were examined by reverse transcription PCR. One day of incubation with the antioxidants reduced the ROS content in the embryos but did not affect the rate of development to the blastocyst stage. Blastocysts developed in medium containing MitoT had lower mitochondrial DNA copy numbers and ATP content, whereas MitoQ showed similar but insignificantly trends. Treatment of embryos with either MitoT or MitoQ decreased the expression levels of NRF2 in the nucleus and levels of PGC1α and TFAM. These findings indicate that reductions in mitochondrial ROS levels are associated with low mitochondrial biogenesis in embryos.

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Effect of PPARGC1A on the development and metabolism of early rabbit embryos in vitro

Cited by 7 publications

References 53 publications

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p

Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos

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