Effect of N‐acetylarginine, a metabolite accumulated in hyperargininemia, on parameters of oxidative stress in rats: protective role of vitamins and L‐NAME

Abstract

In the present investigation, we initially evaluated the in vitro effect of N-acetylarginine on thiobarbituric acid-reactive substances (TBA-RS), total sulfhydryl content and on the activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the blood, kidney and liver of rats. Results showed that N-acetylarginine, at a concentration of 5.0 μM, decreased the activity of CAT in erythrocytes, enhanced TBA-RS in the renal cortex, decreased CAT and SOD activities in the renal medulla and decreased CAT and increased SOD and GSH-Px activities in the liver of 60-day-old rats. Furthermore, we tested the influence of the antioxidants, trolox and ascorbic acid, as well as of the N(ω) -nitro-L-arginine methyl ester (L-NAME) on the effects elicited by N-acetylarginine on the parameters tested. Antioxidants and L-NAME prevented most of the alterations caused by N-acetylarginine on the oxidative stress parameters evaluated. Data indicate that oxidative stress induction is probably mediated by the generation of NO and/or ONOO(-) and other free radicals because L-NAME and antioxidants prevented the effects caused by N-acetylarginine in the blood, renal tissues and liver of rats. Our findings lend support to a potential therapeutic strategy for this condition, which may include the use of appropriate antioxidants for ameliorating the damage caused by N-acetylarginine.

Untargeted metabolomics of prostate cancer zwitterionic and positively charged compounds in urine

“…The carnitine system has an enzymatic role as a crucial factor in cancer metabolic plasticity, so to survive in the face of adverse environmental conditions [67]. Sasso et al showed that Nacelylarginine can reduce the activity of antioxidant enzymes such as catalase, increasing the oxidative stress possibly related to neoplastic induction in different systems [68]. An abundant supply of amino acids is important to sustain cancer proliferative drive and arginine can be an auxotrophy nutrient for different neoplastic cells [69].…”

Metabolic and Metabo-Clinical Signatures of Type 2 Diabetes, Obesity, Retinopathy, and Dyslipidemia

“…Proline is known to protect mammalian cells against oxidative stress [24], and the novel associations of thioproline and N-methylproline are associated only with T2D; thioproline, which is an antioxidant that protects the body from oxidative stress [25], was decreased. Also novel and associated only with T2D were N-acetylarginine, which affects oxidative stress in rats [26], and dopamine sulfate. Dopamine is of special importance in neuronal redox-homeostasis and viability and is linked to oxidative stress; its degradation results in the production of ROS, and its oxidation can lead to endogenous neurotoxins, while some dopamine derivatives also show antioxidative effects [27].…”

Loss of Chloroplast GNAT Acetyltransferases Results in Distinct Metabolic Phenotypes in Arabidopsis

“…Very little to nothing is known about the physiological role and prevalence of free acetylated arginine and proline in plants, which were strikingly decreased in gnat2 and gnat7 plants, respectively ( Table 2 ). In mammals, N α -acetyl- l -arginine has been shown to accumulate when the urea cycle is impaired, and some studies have implicated N α -acetyl- l -arginine in oxidative stress, although the mechanism behind this remains elusive ( Sasso et al. 2014 , Chaleckis et al.…”