EFFECT OF <i>N</i>‐ACETYL‐<scp>l</scp>‐CYSTEINE ON LUNG ISCHAEMIA REPERFUSION INJURY IN A PORCINE EXPERIMENTAL MODEL

Chamogeorgakis, Themistokles; Kostopanagiotou, Georgia; Kalimeris, Constantine A.; Kabouroglou, George I.; Kourtesis, Antonios; Routsi, C.; Dima, Cleanthi; Toumpoulis, Ioannis K.

doi:10.1111/j.1445-2197.2007.04357.x

Cited by 3 publications

(2 citation statements)

References 33 publications

(62 reference statements)

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“…Previous studies have shed some light on several potential therapeutic strategies including the use of aprotinin [4], N-acetyl-L-cysteine [16], hypothermia [17], and inhalational nitric oxide [18]. However, the effectiveness of these treatment modalities is still uncertain.…”

Section: Introductionmentioning

confidence: 99%

Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model

et al. 2011

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BackgroundThis study tested the hypothesis that autologous transplantation of adipose-derived mesenchymal stem cells (ADMSCs) can effectively attenuate acute pulmonary ischemia-reperfusion (IR) injury.MethodsAdult male Sprague-Dawley (SD) rats (n = 24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus intravenous transplantation of 1.5 × 106 autologous ADMSCs at 1h, 6h, and 24h following IR injury). The duration of ischemia was 30 minutes, followed by 72 hours of reperfusion prior to sacrificing the animals. Blood samples were collected and lungs were harvested for analysis.ResultsBlood gas analysis showed that oxygen saturation (%) was remarkably lower, whereas right ventricular systolic pressure was notably higher in group 2 than in group 3 (all p < 0.03). Histological scoring of lung parenchymal damage was notably higher in group 2 than in group 3 (all p < 0.001). Real time-PCR demonstrated remarkably higher expressions of oxidative stress, as well as inflammatory and apoptotic biomarkers in group 2 compared with group 3 (all p < 0.005). Western blot showed that vascular cell adhesion molecule (VCAM)-1, intercellular adhesion molecule (ICAM)-1, oxidative stress, tumor necrosis factor-α and nuclear factor-κB were remarkably higher, whereas NAD(P)H quinone oxidoreductase 1 and heme oxygenase-1 activities were lower in group 2 compared to those in group 3 (all p < 0.004). Immunofluorescent staining demonstrated notably higher number of CD68+ cells, but significantly fewer CD31+ and vWF+ cells in group 2 than in group 3.ConclusionADMSC therapy minimized lung damage after IR injury in a rodent model through suppressing oxidative stress and inflammatory reaction.

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Section: Introductionmentioning

confidence: 99%

Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model

et al. 2011

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“…[33][34][35] One randomized, double-blind, placebo-controlled clinical trial found that NAC did not lead to improvement in clinical results or biochemical markers, with only lower IL-6 levels without significant difference. 36 Another study, however, found that NAC decreased oxidative stress in patients undergoing coronary artery bypass grafting using cardiopulmonary bypass.…”

Section: Discussionmentioning

confidence: 99%

Prevention of primary graft dysfunction in lung transplantation by N-acetylcysteine after prolonged cold ischemia

İnci

Erne

Arni

et al. 2010

The Journal of Heart and Lung Transplantation

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Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats

Saad

Filho

et al. 2013

Journal of Surgical Research

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EFFECT OF N‐ACETYL‐l‐CYSTEINE ON LUNG ISCHAEMIA REPERFUSION INJURY IN A PORCINE EXPERIMENTAL MODEL

Abstract: N-acetyl-L-cysteine when administered directly in the pulmonary artery showed no significant change in haemodynamic and functional lung parameters during ischaemia reperfusion; it does, however, have an indirect surfactant-protective effect.

Cited by 3 publications

References 33 publications

Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model

Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model

Prevention of primary graft dysfunction in lung transplantation by N-acetylcysteine after prolonged cold ischemia

Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats

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