Effect ofMusca domesticmaggot polypeptide extract on HUVEC dysfunction induced by early-activated macrophages

Abstract

Context: Musca domestica Linn. maggot is a traditional Chinese medicine. In our previous studies, Musca domestica maggot protein-enriched fraction and polypeptide extract (molecular weight 530 kD) were found to reverse endothelial cell dysfunction in atherosclerotic lesions. Objective: This study determines whether and how M. domestica maggot polypeptide extract affects the dysfunction of human umbilical vein endothelial cells (HUVEC) induced by macrophages (M'). Materials and methods: HUVEC and early-activated THP-1 M' (incubated with LPS of 1 mg/ml for 2 h) were co-cultured in a Transwell system. The effects of Musca domestica maggot polypeptide extract (with increasing concentrations, i.e., 1.0, 2.5, 5.0, 10.0, 20.0, and 40.0 mg/ml) on the proliferation and migration HUVEC and their secretion of vascular endothelial growth factor (VEGF) were determined by flow cytometry, modified Boyden chamber assay, and enzyme-linked immunosorbent assay (ELISA) after incubation for 24 h. Results: Musca domestica maggot polypeptide extract decreased the proliferation of HUVEC in a concentration-dependent manner, with a 50% effective concentration (EC 50 ) of 22.16 ± 1.48 mg/ml, and effectively inhibited HUVEC migration (EC 50 ¼ 35.15 ± 2.03 mg/ml) and VEGF secretion (EC 50 ¼ 28.64 ± 1.29 mg/ml). Discussion and conclusion: Musca domestica maggot polypeptide extract can inhibit the dysfunction of HUVEC induced by early-activated THP-1 M'.

Terapia Larval con Musca Domestica en el Tratamiento de la úlcera Leishmánicaen un Modelo Murino

“…En el caso de M. domestica hasta la fecha se han detectado ES con actividad antibacterial, antimicrobial, antioxidante, mitogénica, con efecto hepático protector o antitumoral. Otras investigaciones indican que existe actividad biológica de péptidos muy complejas como cecropinas, defensinas y atacinas en las larvas de M. domestica (Andersen et al, 2010;Jin et al, 2010;Wang et al, 2012;Zhu et al, 2013;Pinheiro et al, 2015;Chu et al, 2016), pero se desconoce si existen ES con potencial actividad anti-Leishmania. Por otro lado, en las investigaciones de la TL sobre úlceras leishmánicas en modelos murinos, se ha trabajado no solo con diferentes especies de moscas, sino también con varias especies de Leishmania como L. amazonensis, L. panamensis, L. major y L. tropica, observándose la efectividad de cicatrización y anti-Leishmania de L. sericata sobre L. amazonensis, L. panamensis, L. major y L. tropica.…”

Comparison of the antioxidant activities of nonfumigated and sulphur-fumigated Chrysanthemum morifolium cv. Hang-ju induced by oxidative stress

“…Cell growth and the sensitivity of HUVECs to the two HJ extracts were assessed. The HUVECs were preincubated with varying concentrations of NHJ (50, 100 and 200 lg/mL) and SHJ (50, 100 and 200 lg/mL) for 24 h and then treated with ox-LDL (20 lg/mL) for another 2 h. The cells were collected to test for intracellular ROS, MDA and SOD production as well as cell apoptosis (Yu et al 2010;Chu et al 2015).…”