Effect of <i>Jiawei Fengshining</i> on Synovial Cell Apoptosis and TGF-<i>β</i>1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis

Dong, Xiaorong; Gao, Yuan; Ding, Lina; Zeng, Fujia; Ding, Dou

doi:10.1155/2019/8614034

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…Except for the control group ( n = 8), each rat was injected with 0.2 mL the mixture under the plantar fascia of the left hind foot to get the CIA rat model. The injection volume is 0.1 mL/mouse, and each animal is injected once [ 16 , 17 ]. On the 7th day after the inflammation, the foot and plantar volume were measured.…”

Section: Methodsmentioning

confidence: 99%

The protective effect of gentisic acid on rheumatoid arthritis via the RAF/ERK signaling pathway

et al. 2022

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Background RAF and ERK pathways are known to be activated in human rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS), which play an important role in the pathogenesis and destruction of RA. Gentisic acid (GA) was a natural product derived from plants, which has been reported can attenuate pressure overload‐induced cardiac hypertrophy and fibrosis in mice through inhibition of the ERK1/2 pathway. Whether GA can inhibit the occurrence and development of RA through RAF/ERK signaling pathway has not been reported. The purpose of this study is to determine whether GA may have a certain therapeutic effect on RA-FLS. Method Bovine type II collagen was used to establish a rat model of rheumatism. Enzyme-linked immunosorbent assay was used to detect inflammatory factors, anti-inflammatory mediators, and rheumatoid factor. Hematoxylin and eosin and TUNEL staining were used to detect the effect of GA on histochemical with rheumatoid arthritis. RAF, ERK, and p-ERK expressions in synovial tissue were measured by western blot and immunohistochemical. Besides, human rheumatoid arthritis fibroblast-like synoviocytes cell line MH7A was used to investigate the biological behavior influenced by GA. Apoptosis assay was performed to detect apoptosis of GA on MH7A cells. Transwell invasion assay was performed to detect the ability of cell migration. Result The result showed that GA could reduce joint swelling and inflammation. At the same time, it can also promote the apoptosis of synovial cells and down-regulate the RAF/ERK pathway. Conclusion GA may ameliorate inflammatory factors’ abnormality, synovial hyperplasia, and apoptosis of synovium via inhibiting the RAF/ERK signaling pathway.

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Section: Methodsmentioning

confidence: 99%

The protective effect of gentisic acid on rheumatoid arthritis via the RAF/ERK signaling pathway

et al. 2022

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“…It not only causes great sufering for patients and family members but also brings heavy fnancial burden for individuals and society [5]. Drugs that reduce joint pain or retard the progression of disease include nonsteroidal antiinfammatory drugs (NSAIDs), disease-modifying antirheumatic drugs (DMARDs), and steroids and biological response modifers [6]. However, these drugs can produce serious side efects, including renal toxicity, gastrointestinal toxicity, hepatic damage, and myelosuppression [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology and Molecular Docking Explore the Mechanism of Mubiezi‐Yinyanghuo Herb Pair in the Treatment of Rheumatoid Arthritis

Meng,

Tao,

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Objective. Our previous studies have shown that the Mubiezi-Yinyanghuo (MBZ-YYH) herb pair inhibits rheumatoid arthritis (RA) cell proliferation and glycolysis, promising results with an obscure mechanism of action. Methods. Therefore, it is necessary to explore the main components of MBZ-YYH and unravel the potential mechanism in RA based on network pharmacology and molecular docking methods. Components and targets of MBZ-YYH were retrieved from the TCMSP. Relevant targets of RA were searched in GeneCards, therapeutic target database (TTD), and DisGeNET databases; the common targets of the MBZ-YYH compounds and RA were obtained by comparison; and a component-target interaction network was established by Cytoscape 3.9.1. Gene ontology (GO) analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis were performed through the David database. Molecular docking was performed by PyMoL2.3.0 and AutoDock Vina1.1.2 software. Results. 7 active ingredients and 58 putatively identified target genes were screened from MBZ, and 16 effective components of YYH and 230 potential targets were identified. There were 29 mutual targets between the two herbs and RA. Through the PPI network, 9 hub targets which contain JUN, CASP3, PPARG, PTGS2, GSK3B, CASP8, HMOX1, ICAM1, and HK2 were screened out. GO term enrichment analysis indicated that positive regulation of the apoptotic process, response to drugs, and response to hypoxia were significantly enriched. Based on KEGG analysis, it was mainly associated with the IL-17 signaling pathway, the TNF signaling pathway, and the p53 signaling pathway. The docking analysis revealed that the effective components showed strong binding activity with the receptors. Conclusion. The effects of the MBZ-YYH herb pair on RA were coordinated by the interaction of diverse components, which may be through the IL-17 signaling pathway and the TNF signaling pathway, which target GSK3B, HK2, caspase 3, and caspase 8, inhibiting the proliferation and glycolysis of rheumatoid arthritis fibroblast-like synovial cells (RA-FLS) and tending towards an increasing efficacy and decreasing toxicity effect on RA.

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Gaultheria leucocarpa Blume Ericaceae

Mustaqim¹,

Setiawan

2020

Ethnobotany of Mountain Regions

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Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-β1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis

Cited by 7 publications

References 38 publications

The protective effect of gentisic acid on rheumatoid arthritis via the RAF/ERK signaling pathway

The protective effect of gentisic acid on rheumatoid arthritis via the RAF/ERK signaling pathway

Network Pharmacology and Molecular Docking Explore the Mechanism of Mubiezi‐Yinyanghuo Herb Pair in the Treatment of Rheumatoid Arthritis

Gaultheria leucocarpa Blume Ericaceae

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