Effect of <i>In Vitro</i> Culture of Mammalian Embryos on the Architecture of the Zona Pellucida

Silva, C P; Silva, V; Kommineni, Karpura; Keefe, David L.

doi:10.1086/bblv193n2p235

Cited by 15 publications

(14 citation statements)

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“…Those issues include the zona hardening because of suboptimal culture conditions in vitro [24], natural zona thickness [7] and zona thickening following cryopreservation [25,26]. It has been reported in literature that implantation rate per embryo transfer for blastocsyst transfers was 23-25% [27].…”

Section: Discussionmentioning

confidence: 99%

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

Kutlu¹,

Atvar²,

Vanlioglu³

2010

J Assist Reprod Genet

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Purpose Laser assisted zona thinning is a technique used for facilitating embryo's hatching process and is commonly used for the embryos of poor prognosis patients with advanced maternal age, repeated implantation failures, poor embryo quality or thick zona pellucida. The aim of this study was to investigate the effect of zona thinning on both good or poor prognosis patients of two different age groups. Methods We investigated two different age groups (group 1: <35 years and group 2: ≥35 years ) and compared the effect of assisted zona thinning (groups 1A and 2A) versus not-thinned controls (groups 1B and 2B) in both groups.Results The clinical pregnancy rates were 57% and 56% in groups 1A and 1B (p=0.86), 43% and 38% in groups 2A and 2B (p=0.59) respectively. Conclusions Our results suggest that laser assisted zona thinning of day 3 embryos has no beneficial effect on clinical pregnancy and implantation outcomes.

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Section: Discussionmentioning

confidence: 99%

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

Kutlu¹,

Atvar²,

Vanlioglu³

2010

J Assist Reprod Genet

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“…However, the in vitro culture environment may induce structural changes in the ZP, making it thicker, more compact, less porous, and more resistant to hatching, accordingly hampering further development (Silva et al 1997;Fong et al 2001). Therefore, removing ZP could in fact be beneficial in species where hatching is difficult, such as pigs (Vejlsted et al 2006).…”

Section: Cloning-based Methods: Tc and Hmcmentioning

confidence: 99%

Genetically modified pigs for biomedical research

Luo

Lin

Bolund

et al. 2012

J of Inher Metab Disea

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During the last two decades, pigs have been used to develop some of the most important large animal models for biomedical research. Advances in pig genome research, genetic modification (GM) of primary pig cells and pig cloning by nuclear transfer, have facilitated the generation of GM pigs for xenotransplantation and various human diseases. This review summarizes the key technologies used for generating GM pigs, including pronuclear microinjection, sperm‐mediated gene transfer, somatic cell nuclear transfer by traditional cloning, and somatic cell nuclear transfer by handmade cloning. Broadly used genetic engineering tools for porcine cells are also discussed. We also summarize the GM pig models that have been generated for xenotransplantation and human disease processes, including neurodegenerative diseases, cardiovascular diseases, eye diseases, bone diseases, cancers and epidermal skin diseases, diabetes mellitus, cystic fibrosis, and inherited metabolic diseases. Thus, this review provides an overview of the progress in GM pig research over the last two decades and perspectives for future development.

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“…The inner layer showed the highest birefringence with radially arranged filaments and the outer layer had a moderate birefringence due to the tangential orientation of its filaments which decreased the light to a lower degree. The medium layer presents birefringence due to irregular position of its filaments (Rama Raju et al, 2007;Silva et al, 1997). Figures 1 and 2 demonstrate the differences between high birefringence (positive value +3.5) and low birefringence (negative value, -8,7).…”

Section: Birefrigence Of the Zona Pellucidamentioning

confidence: 99%

Zona pellucida birefringence evaluation

Kreuscher¹,

Rosa²,

Neto³

et al. 2013

JBRA Assisted Reproduction

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RESUmOObjetivo: Este trabalho teve como objetivo fazer uma revisão bibliográfica dos trabalhos que abrangem assuntos sobre a birrefringência da zona pelúcida. materiais e métodos: Levantamento bibliográfico em revistas científicas tais como: Fertility Sterility; Human Reproduction, Reproduction BioMedicine Online; Revista Brasileira de Análises Clínicas; Revista Brasileira de Ginecologia e Obstetrícia; o manual de procedimentos da Red Latinoamericana de Reproducción Asistida; entre outros. A variação de data de busca nos artigos selecionados foi de dez anos (2001 a 2011). Revisão de Literatura:oócito são uma prioridade para a execução da técnica de Reproduçao Humana Assistida. A birrefringência da zona com o maior potencial de desenvolvimento para se tornar um embrião com maiores chances de desenvolvimento, sendo que a camada interna da zona pelúcida pode ser proposta como marcador de qualidade do oócito. Discussão: Algumas pesquisas concluíram que oócitos com alta birrefringência tem maiores chances de fertilização e que a birrefringência da zona pelúcida diminui à medida que as fases de maturação oocitária ocorrem. Conclusão: Esta revisão mostrou um possível potencial benéfico da luz polarizada na avaliação da birrefringência da zona pelúcida, podendo portando ser utilizada como preditor da qualidade oocitária. Palavras-chave: infertilidade, FIV, ICSI, birrefringên-cia, zona pelúcida.

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Effect of In Vitro Culture of Mammalian Embryos on the Architecture of the Zona Pellucida

Cited by 15 publications

References 0 publications

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

Genetically modified pigs for biomedical research

Zona pellucida birefringence evaluation

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