Effect of Ginkgo biloba Extract on Rehabilitation Treatment in Patients with Sepsis

Abstract

Ginkgo biloba extract (GBE), as a natural herbal medicine, is thought to play a positive role in the rehabilitation of sepsis. This article aimed to explore the application effect of GBE in the rehabilitation treatment of patients with sepsis. Firstly, the system applicability test and chromatographic conditions were used to determine the content of GBE, the content was determined after solution preparation, and the precision of GBE was tested after sample collection to analyze the composition of GBE. 70 patients with sepsis admitted to hospital were retrospectively analyzed. The patients were divided into Controls (conventional treatment, n = 35) and GBE group (GBE treatment, n = 35). Through treatment, the levels of procalcitonin (PCT), interleukin-33 (IL-33), sequential organ failure assessment (SOFA), cTn1, CK-MB, IL-6, and TNF-α were compared between the two groups. H9C2 cells were cultured and treated. Normal group was cultured in DMEM medium having FBS without treatment. H2O2 group was treated with DMEM medium having FBS with 100 μmol/L H2O2 solution. H2O2 + GBE group was treated with 100 μmol/L H2O2 solution and 15 μmol/L ginkgetin solution. Western blot was used to detect the protein expression of Caspase-3, Caspase-6, Bax/Bcl-2, and FOXO1. It revealed the RSD of quercetin, kaempferol, and isorhamnetin in GBE was 0.51%, 0.92%, and 1.32%, respectively, less than 2.0%. Sample content determination indicated that the dried products met the requirements. PCT and SOFA, and the level of IL-33 in GBE group was clearly higher as against Controls, and cTnI, CK-MB, IL-6, and TNF-α in the Controls were clearly higher as against the GBE group (P < 0.05). In vitro cell experiments suggested that the Bax/Bcl-2 ratio and the expression of Caspase-3, Caspase-6, and FOXO1 proteins were gradually increased in Normal, H2O2, and H2O2 + GBE groups. The H2O2 group was clearly superior as against the Normal, and the H2O2 + GBE group was clearly superior as against the H2O2 group (P < 0.05). As against the Normal, the expressions of Caspase-3 and Caspase-6, and the ratio of Bax to Bcl-2 was clearly decreased in the H2O2 + GBE group (P < 0.05). In conclusion, GBE can promote the recovery process of patients by reducing inflammatory response, inhibiting oxidative stress, and improving immune function.