Effect ofFasciola giganticaexcretory secretory antigen on rat hematological indices

Abstract

The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37℃, and centrifuging the supernatant at 12,000 g at 4℃ for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats.

Consumption Hazards of Cattle Liver Infected With Fasciola Spp.: Ii. Experimental Study on Rats

“…Rats fed on heavily infected livers showed the start of necrosis and hydropic degeneration of some hepatocytes, caused by the waste product effects of Fasciola worms or their excretory products on the infected cattle liver. This coincides with Ganga et al (2006) as the metabolites discharged by the parasite in situ may be a cause of pathological lesions in the liver and anemia. The role of amino acid (proline) secreted by Fasciola proved to be toxic to the host and cause anemia by destroying red blood cells (Ganga et al, 2006;Adarosy et al, 2013).…”

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

“…The developing F. gigantica juveniles and the adults have been considered non-haematophagous and tissue feeding parasites yet cause severe anaemia in the host suffering from acute and/or chronic disease (Ganga et al 2004b). Thus, pathogenesis of anaemia in fasciolosis has therefore been debatable.…”

Cloning and characterization of a novel cathepsin B-like cysteine proteinase from Angiostrongylus cantonensis

“…Proteolytic enzymes are essential components of biological systems and play key roles in the development of parasitic diseases (Cho et al 1999;Ganga et al 2006). Releasing as excretory-secretory antigens, the production of proteolytic enzymes has been reported for various helminths (McKerrow et al 1990;Na et al 2006;Ma et al 2007;Zheng et al 2011).…”