Effect of<i>Eclipta prostrata</i>on 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

Xu, Chenshu; Bing-hua, Wei; Fu, Xiaohua; Luo, Mei-Juan; Liu, Shidai; Li, Ruiming; Ren, Bin; Tang, Lei

doi:10.1155/2014/651053

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…The main features of NS are massive proteinuria, hypoproteinemia, hyperlipidemia and varying degrees of edema 5–7 . Clinically, glucocorticoids are commonly used to treat NS 8 . Eclipta prostrata , recorded in the Chinese Pharmacopoeia, is the dry airel part of the Compositae Eclipta prostrata L 9 .…”

Section: Introductionmentioning

confidence: 99%

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

Wang¹,

Huang²,

Li³

et al. 2020

Phytochemical Analysis

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Introduction: The diversity and complexity of components are important reasons for the unstable efficacy and safety of Chinese materia medica (CMM) in quality control. The good and stable quality control may be related to the quality markers with structural composition of multi-components. Objective: In the present study, we take Eclipta prostrata L. as a representative example. The 11 samples were collected from the different areas in China, and the discrepancy in bioactivity and chemical composition of these samples were compared. DOX (doxorubicin hydrochloride)-induced ICR mice were established for in vivo nephrotic syndrome experiments. The biochemical indicators including 24-h urine protein, triglyceride (TG), etc. were measured and the pathological change of kidney tissue was observed. MPC-5 cells damage model was induced to compare the difference of these samples in bio-activity. High-performance liquid chromatography (HPLC) method for 11 EEP (extract of Eclipta prostrata L.) samples were performed to analyse the content of the quality markers. Results: In vivo experiments, EEP could mitigate the content or activity of urine protein, TG, etc. compared with the positive group (TG content was 2.53 ± 0.39 mmol/L, urinary protein quantification on 35th day was 16.79 ± 2.32 mg). In vitro experiments, CCE (coumestans component of Ecliptae) and EEP had equivalent effects on biochemical indicators such as cell viability, etc. According to the HPLC analysis, the content of wedelolactone was 45.88% and demethylwedelolactone was 23.74% in CCE. Conclusion: The CCE with a ratio of 2:1 can be considered as a quality marker of Eclipta prostrata L.. This research may provide a perspective for quality control of CMM.

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Section: Introductionmentioning

confidence: 99%

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

Wang¹,

Huang²,

Li³

et al. 2020

Phytochemical Analysis

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“…In the current study, the prednisolone concentration was decreased in kidney, testes, muscle and fat tissues in DMC pre-treated rats, indicating the effect of DMC in regulating tissue distribution of prednisolone, which may attribute to the reduced adverse drug reactions of glucocorticoid by DMC. Recently reported data from our laboratory reveal that the enzyme activity, gene and protein expression of rat kidney 11β-HSD II are reduced by oral administration of Eclipta prostrata, one of the major constituents of DMC (Xu et al, 2014). The effect of DMC in reducing adverse drug reactions of glucocorticoid may thus be regulated through 11β-HSD enzymes.…”

Section: Discussionmentioning

confidence: 94%

Tissue Distribution of Prednisone/Prednisolone is Affected by TCM Danmo Capsule in Rats

Zhang¹,

Bing-hua²,

Xia³

et al. 2015

International J. of Pharmacology

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A B S T R A C T Danmo Capsule (DMC) is a patent Chinese botanic drug, widely used in combinational therapy with prednisone in China to treat renal diseases. The combined therapy regimen can reduce the dosage of glucocorticoids and relieve symptoms of glucocorticoid related adverse drug effects. Previous study from our laboratory showed that co-administration of the DMC significantly increased the plasma C max and AUC of glucocorticoid in rats. However, the effect of DMC on prednisone/prednisolone tissue distribution remains unclear. Therefore, the purpose of the current research was to study the tissue distribution of prednisone/prednisolone in rats after DMC was co-administered. Rats were treated with control vehicle or DMC (0.432 g kgG 1 ) for 14 days before prednisone acetate (42 mg kgG 1 ) was orally given. Samples from the heart, liver, spleen, lung, kidney, brain, fat, muscle, testes, small intestine and thymus were collected at different time points. The concentrations of prednisone and prednisolone were determined by high-performance liquid chromatography. Compared with prednisone alone group, the prednisone concentrations in rat lung, kidney, heart, muscle, small intestine and fat were increased in DMC treated groups, while those in liver, spleen, brain and thymus were decreased. However, the prednisolone concentrations in liver, lung, small intestine and thymus were increased, while those in kidney, fat, muscle, testes and heart were decreased following DMC pre-treatment. These results indicated that the alteration of prednisone/prednisolone levels in important tissues, when DMC was co-administered, may contribute to the effect of DMC in reducing adverse drug reactions of glucocorticoid and lowering its dosage administered.

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“…In the previous study, we proved that Eclipta prostrata (EP) significantly affected the activity and expression of 11 β -HSD enzymes, which might improve the efficacy and reduce the adverse drug effects of glucocorticoid in patients undergoing combinational therapy [ 18 ]. However, to the best of our knowledge, no further investigation has been performed to show which composition of EP plays a major role and how it works.…”

Section: Introductionmentioning

confidence: 99%

Effect ofLuteolinon 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

Tang

Deng

Shi

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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11Beta-hydroxysteroid dehydrogenase (11β-HSD) enzymes control the glucocorticoid (GC) signaling, which is essential in regulating homeostasis. Our previous study revealed that Eclipta prostrata (EP) affected the activity and expression of 11β-HSD enzymes which might improve the efficacy and reduce the adverse drug effects of glucocorticoid in patients undergoing combinational therapy. However, it is still unclear which composition of EP plays a major role and how it works. In this paper, we chose Luteolin which is one of the main ingredients of EP and evaluated its effect and metabolism in combination with prednisone. The effects of different concentrations of Luteolin extract on prednisone/prednisolone metabolism indicated the enzyme activity of 11β-HSD, so the production rate (pmol/min per mg protein) of metabolites was used to indicate enzyme activity. Furthermore, we explored the influence of Luteolin on gene and protein expressions of 11β-HSD I/II in rat liver and kidney tissue. Our results showed that oral administration of Luteolin significantly increased the gene and protein expressions of hepatic 11β-HSD I and renal 11β-HSD II, which may improve the efficacy and reduce the adverse drug effect of glucocorticoid in clinical application. A potential clinical value of Luteolin would also be indicated in combination therapy with prednisone for the treatment of nephrotic syndrome.

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Effect ofEclipta prostrataon 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

Cited by 3 publications

References 29 publications

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

The combination of HPLC and biological analysis to determine the quality markers and its structural composition of Eclipta prostrata L.

Tissue Distribution of Prednisone/Prednisolone is Affected by TCM Danmo Capsule in Rats

Effect ofLuteolinon 11Beta-Hydroxysteroid Dehydrogenase in Rat Liver and Kidney

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