2014
DOI: 10.1111/aab.12110
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Effect of Dionaea muscipula extract and plumbagin on maceration of potato tissue by Pectobacterium atrosepticum

Abstract: Pectobacterium atrosepticum (Pba) is a plant pathogen that causes major crop losses. Dionaea muscipula extracts and their antibacterial constituent, plumbagin, inhibit Pba growth in vitro. However, this effect is reduced when the extracts are added to bacterial cultures present on potato tubers or suspended in potato tuber filtrate (PF). To explain this, we examined the response mechanism of Pba cells to Dionaea extract and plumbagin and compared it with the effect of a bactericidal peptide -CAMEL. The additio… Show more

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Cited by 7 publications
(3 citation statements)
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“…The source of ChPL were 8-week-old Dionaea muscipula plants cultured in vitro according to a previously published procedure (Szpitter et al, 2014).…”
Section: Methodsmentioning
confidence: 99%
“…The source of ChPL were 8-week-old Dionaea muscipula plants cultured in vitro according to a previously published procedure (Szpitter et al, 2014).…”
Section: Methodsmentioning
confidence: 99%
“…After 24 h incubation at 28°C (48 h at 21°C for Cms), the lowest concentration of caffeine inhibiting bacterial growth was designated as the minimum inhibitory concentration (MIC). The minimum bactericidal concentration (MBC) was defined as the lowest concentration of caffeine that eliminated 99.9% of bacterial cells within 24 h (Thornsberry, 1991;Szpitter et al, 2014). Experiments were performed in triplicate.…”
Section: Methodsmentioning
confidence: 99%
“…Effect of caffeine on DNA replication, RNA and protein biosynthesis in bacterial cells. The procedures of Wegrzyn et al (1991) and Szpitter et al (2014) were followed. Briefly, Dsol, Rsol, Pba, Pcc, Cms, Pst, Xcc suspensions were adjusted to OD 580 = 0.1.…”
Section: Methodsmentioning
confidence: 99%