Effect of Carica papaya Extract toward Incised Wound Healing Process in Mice (Mus musculus) Clinically and Histologically

Abstract

Wound healing entails a sequence of complex biological processes, which is a protective function of the body that focuses on a quick recovery. Reducing wound healing time is crucial in a wound as it lowers the chance of infection and decreases complications and costs. Papaya extract was obtained by a maceration method. It contains papain enzyme, flavonoid, saponin, and alkaloid, which act as an anti-inflammatory, astringent substance, vasodilator, antioxidant, analgesic, with antifungal, and antibacterial properties, and increase the collagen synthesis. This study aimed to assess the effect of Carica papaya extract application toward incised wound healing process in mice (Mus musculus) clinically and histologically. This experimental laboratory study was performed using 24 mice weighing between 30 and 40 grams and 12 and 14 weeks of age as experimental animals. Each group was incised along 5 mm at the labial gingiva under both of mandible anterior teeth with a depth reaching alveolar bone. Papaya extract was applied topically in the morning and evening for 14 days. The clinical result was obtained by assessing the length of wound closure measured every day for 14 days. Based on the statistic test result, it showed that the papaya extract has a significant effect (p ≤ 0.05) toward the healing process of an incised oral wound in mice. Histologically at day 14, 75% concentration papaya extract showed perfect epithelial layer and fibrillation.

Beneficial Role of Carica papaya Extracts and Phytochemicals on Oxidative Stress and Related Diseases: A Mini Review

“…The concepts were further supported by in vivo studies evaluating different parts of Carica papaya on incised, burned, and diabetic wounds respectively. The recent findings show that Carica papaya fruit and seed extracts demonstrated dose-dependent increment in hydroxyproline, fibrillation, epithelial thickness, shortened wound contraction, and epithelialization time [ 69 , 70 , 71 , 72 ].…”

“…Cysteine endopeptidases including papain and chymopapain showed wound healing activity that can be attributed to their proteolytic wound debridement and antibacterial effects [ 63 , 64 , 70 , 72 ]. This was established by an in vivo study using papain-based wound cleanser.…”

Effect of a Gel Based on Ipomoea batatas (Purple Sweet Potato) on Dermal Wound Healing in Mice

“…The faster healing time reduces the possibility of complications in the injury, these in turn reduce the costs that may be involved in prolonging the treatment. 21 In the histopathological analysis of the groups that received the purple sweet potato gels, an active recovery of the stratum corneum, granular, spinous and basal cells is shown. These later will be in charge of replacing the dead epidermal cells.…”

The New Challenge of Green Cosmetics: Natural Food Ingredients for Cosmetic Formulations

“…Caffeic acid reduces skin erythema via inhibitory action towards NF-κB and AP-1 signaling [ 205 ]. Cysteine endopeptidases and chymopapain have proteolytic wound-debridement and antibacterial effects [ 206 , 207 , 208 , 209 ].…”