Effect of
            <i>Bradyrhizobium</i>
            photosynthesis on stem nodulation of
            <i>Aeschynomene sensitiva</i>

Giraud, Éric; Hannibal, Laure; Fardoux, Joël; Vermeglio, André; Dreyfus, B

doi:10.1073/pnas.250484097

Cited by 90 publications

(79 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…strain ORS278 (ref. 5). This strain forms nitrogen-fixing nodules on the stems of the plant Aeschynomene sensitiva, a tropical legume that grows in water logged soils 6 .…”

Section: Phototrophymentioning

confidence: 99%

Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris

et al. 2003

View full text Add to dashboard Cite

“…strain ORS278 (ref. 5). This strain forms nitrogen-fixing nodules on the stems of the plant Aeschynomene sensitiva, a tropical legume that grows in water logged soils 6 .…”

Section: Phototrophymentioning

confidence: 99%

Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris

et al. 2003

View full text Add to dashboard Cite

“…The 4.7-kb SalI lacZ-Km r cassette of pKOK5 was then inserted into the SalI site designed in the overlapping sequences of the up.pucBA.R and dw.pucBA.F primers (in italics). These two constructions were introduced and delivered by conjugation into the BTAi1 strain as previously described (13). Double recombinants were selected on sucrose and confirmed by PCR.…”

Section: Methodsmentioning

confidence: 99%

“…After growth, the cells under the illuminated area were resuspended in 3 ml of water and ␤-galactosidase activity was measured as previously described (13).…”

mentioning

confidence: 99%

Control of Peripheral Light-Harvesting Complex Synthesis by a Bacteriophytochrome in the Aerobic Photosynthetic Bacterium Bradyrhizobium Strain BTAi1

et al. 2008

Self Cite

View full text Add to dashboard Cite

The recent sequence analysis of the photosynthetic and plant-symbiotic Bradyrhizobium sp. strain BTAi1 revealed the unexpected presence of a pucBA operon encoding the apoproteins of peripheral light-harvesting (LH) complexes. This pucBA operon is found close to a bacteriophytochrome gene (BphP3 B BTAi1 ) and a two-component transcriptional regulator gene (TF BTAi1 gene). In this study, we show that BphP3 B BTAi1 acts as a bona fide bacteriophytochrome and controls, according to light conditions, the expression of the pucBA operon found in its vicinity. This light regulatory pathway is very similar to the one previously described for chromo-BphP4 Rp in Rhodopseudomonas palustris and conducts the synthesis of a peripheral LH complex. This LH complex presents a single absorption band at low temperature, centered at 803 nm. Fluorescence emission analysis of intact cells indicates that this peripheral LH complex does not act as an efficient light antenna. One putative function of this LH complex could be to evacuate excess light energy in order to protect Bradyrhizobium strain BTAi1, an aerobic anoxygenic photosynthetic bacterium, against photooxidative damage during photosynthesis.Anoxygenic phototrophic bacteria have the ability to transform light energy into biochemical amenable energy for their growth and motion. The collection of light and its transformation into chemical energy are mediated by the so-called photosynthetic apparatus. This complex system is composed of three multimeric transmembrane protein complexes: the lightharvesting (LH) complexes, the photochemical reaction center (RC), and the cytochrome bc 1 complex located in the intracytoplasmic membrane. Light collected by the peripheral LH complexes is transferred first to the LH1 complex, which absorbs at around 870 nm, and then to the RC, where a charge separation occurs. This initiates a cyclic electron transfer between the RC and cytochrome bc 1 via electron carrier proteins in the periplasmic space and quinone molecules in the membrane. This cyclic electron transfer is coupled to the translocation of protons and to the formation of a proton motive force across the inner membrane, ultimately used for ATP synthesis. To optimize light collection, various peripheral LH complexes, coded by different pucBA genes, are expressed according to environmental conditions. In most cases, peripheral LH complexes absorb at 800 and 850 nm and are designated LH2. However, other peripheral LH complexes, which differ by their absorption properties and carotenoid content, have been described. At low light intensities and/or low temperatures, Rhodopseudomonas acidophila synthesizes an LH complex, designated LH3, which absorbs at 800 and 820 nm (4, 8). An atypical LH complex (LH4), presenting a single band around 805 nm, is synthesized by Rhodopseudomonas palustris cells when grown at a low light intensity (16).In general, the photosynthetic activity of anoxygenic phototrophic bacteria takes place under anaerobic or semiaerobic conditions, since the synthesis of the ph...

show abstract

“…Bacterial Strains and Growth Conditions-Bradyrhizobium strain ORS278 was grown in a modified YM agar medium at 35°C for 7 days (19).…”

Section: Methodsmentioning

confidence: 99%

“…The 4.7-kb SalI lacZ-kmR cassette of pKOK5 was then inserted into the SalI site designed in the overlapping sequences of upgvpA.R and dwgvpA.F primers (in italic). These two constructions were introduced and delivered by conjugation into ORS278 strain as described (19).…”

Section: Methodsmentioning

confidence: 99%

A Singular Bacteriophytochrome Acquired by Lateral Gene Transfer

Jaubert

Lavergne

Fardoux

et al. 2007

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Bacteriophytochromes are phytochrome-like proteins that mediate photosensory responses in various bacteria according to their light environment. The genome of the photosynthetic and plant-symbiotic Bradyrhizobium sp. strain ORS278 revealed the presence of a genomic island acquired by lateral transfer harboring a bacteriophytochrome gene, BrBphP3.ORS278, and genes involved in the synthesis of phycocyanobilin and gas vesicles. The corresponding protein BrBphP3.ORS278 is phylogenetically distant from the other (bacterio)phytochromes described thus far and displays a series of unusual properties. It binds phycocyanobilin as a chromophore, a unique feature for a bacteriophytochrome. Moreover, its C-terminal region is short and displays no homology with any known functional domain. Its darkadapted state absorbs maximally around 610 nm, an unusually short wavelength for (bacterio)phytochromes. This form is designated as Po for orange-absorbing form. Upon illumination, a photo-reversible switch occurs between the Po form and a red (670 nm)-absorbing form (Pr), which rapidly backreacts in the dark. Because of this instability, illumination results in a mixture of the Po and Pr states in proportions that depend on the intensity. These uncommon features suggest that BrBphP3.ORS278 could be fitted to measure light intensity rather than color.

show abstract

Effect of Bradyrhizobium photosynthesis on stem nodulation of Aeschynomene sensitiva

Cited by 90 publications

References 39 publications

Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris

Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris

Control of Peripheral Light-Harvesting Complex Synthesis by a Bacteriophytochrome in the Aerobic Photosynthetic Bacterium Bradyrhizobium Strain BTAi1

A Singular Bacteriophytochrome Acquired by Lateral Gene Transfer

Contact Info

Product

Resources

About