Effect of Agave americana L. on the human, and Aspergillus oryzae S2 α-amylase inhibitions

Abstract

Among phenolic compounds, Agave americana L. extract contained puerarin (38.4%) and p-coumaric acid (12.29%) (pCa). From the Lineweaver-Burk plots, pCa and puerarin demonstrated a competitive and a non competitive inhibitions towards human α-amylase activity, respectively. PCa exhibited a higher human inhibitory activity with an IC of 98.8 μM which was about 2.3 times than acarbose. Puerarin (IC = 3.87 μM) and pCa (IC = 10.16 μM) also showed an excellent inhibition for Aspergillus oryzae S2 α-amylase activity. The inhibitions of the described biocatalysts compounds towards both amylases were significantly decreased when they were pre-incubated with starch. The binding modes of these compounds were evaluated in silico. The binding efficiency order of these molecules in terms of polar contact numbers for both enzymes was in agreement with the in vitro studies. These findings provided a rational reason to establish the isolated compounds capability as therapeutic target for hyperglycaemia modulation and antifungal therapy.

A Review of the Effects of Puerarin on Glucose and Lipid Metabolism in Metabolic Syndrome: Mechanisms and Opportunities

“…In the study of Zhao and Zhou, GLUT4 transposition under insulin stimulation and puerarin intervention was observed by an immunofluorescence method. Their result confirmed that the transposition obstacle did happen in the free fatty acid (FFA)-induced IR model of 3T3-L1 lipocytes and that puerarin showed a promoting effect on GLUT4 transposition [ 25 ].…”

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

“…Puerarin and apigenin isolated from this species showed greater inhibitory effects against A. oryzae α-amylase, while p-coumaric acid proved a major inhibitor to human α-amylase at IC 50 : 98.8 µM, with an inhibition rate 2.3 times higher than acarbose. Thus, isolated flavonoids may be used as therapeutic agents for postprandial glycemia [52,53].…”

Extraction of Inulin from Andean Plants: An Approach to Non-Traditional Crops of Ecuador

“…Free fructose was determined by directly measuring the extract, and total fructose was determined by measuring the hydrolyzed extract. Once the inulin content was measured, inulin yield in the extract was determined with Equation (3).…”

“…A variety of Ecuadorian endemic plants were traditionally used due to their medicinal properties, among which jicama (Smallanthus sonchifolius) and cabuya (Agave americana) [1][2][3]. From these Andean species, some autochthonous products have been obtained, for example, "Tshawar mishki" (also known as "agua miel" or honey water), jicama syrup and cabuya syrup [4][5][6].…”