Effect of hypoxia/reoxygenation on the contractility of the isolated bovine digital vein

Risso, Arnaldo; Comerma-Steffensen, Simón; Rojas, Jorge; Ascanio, Elías; Hahn, Michael G.; Reggio, M.; Zerpa, Héctor

doi:10.1016/j.rvsc.2010.11.007

Cited by 2 publications

(4 citation statements)

References 26 publications

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“…If lesions were macroscopically evident, then the distal forelimb was discard. The skin was then reflected over the dorsal area of the digits to reveal the second common digital vein (Risso et al., ) that was dissected and removed, placed in ice‐cold modified Krebs Henseleit (Krebs solution) and transported to the laboratory. The veins were carefully cleared of connective tissue and cut into rings of 4–5 mm in length and used on the same day.…”

Section: Methodsmentioning

confidence: 99%

“…Eight vessel rings could be assessed simultaneously within the course of the experiments. Bovine digital veins rings were stretched to 2 g of basal tension, and an equilibration period of 1 hr was allowed prior to the administration of the selected agent (Risso et al., ). The Krebs solution was changed every 15–30 min during the equilibration period.…”

Section: Methodsmentioning

confidence: 99%

“…Risso et al. () reported an endothelium‐independent hypoxic‐venoconstriction in the isolated bovine digital vein. The participation of the Rho kinase pathway in the 5‐HT enhanced contraction after an in vitro experimental model of long‐term endothelial denudation was reported in the bovine digital vein (Punzi, Belloli, Gogny, Desfontis, & Mallem, ).…”

Section: Introductionmentioning

confidence: 99%

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Endothelium‐dependent relaxation mechanisms involve nitric oxide and prostanoids in the isolated bovine digital vein

Comerma-Steffensen

Risso

Ascanio-Evanoff

et al. 2019

Vet Pharm & Therapeutics

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Endothelial dysfunction contributes to the development of ungulate's laminitis. Although extensively studied in equines, the endothelial function is not fully examined in bovine digital veins (BDVs). BDVs were studied under isometric conditions to describe the acetylcholine (ACh) endothelium‐dependent relaxation. Concentration–response curves were constructed to phenylephrine, ACh, and sodium nitroprusside (SNP). Relaxation responses were evaluated using either phenylephrine or depolarizing high‐potassium Krebs solution (DKS) as precontraction agents. Endothelium denudation and incubation with L‐NAME (300 μM), indomethacin (10 μM) or both were used to explore endothelial‐mediated mechanisms. Endothelium denudation did not modify phenylephrine and SNP responses, however, significantly (p < 0.05) converted a relaxation (63.2 ± 5%) response to ACh into a contraction (30.3±9%). The ACh‐evoked relaxation was significantly (p < 0.05) reduced in the presence of indomethacin (37.5 ± 6%) and L‐NAME (6.40 ± 2%). The presence of both inhibitors abolished the ACh‐evoked relaxation. Although DKS caused a higher precontraction than phenylephrine, ACh‐evoked relaxation (22.4 ± 3.4%) was still observed and was reduced by the combination of inhibitors (7.0 ± 1.0%). The ACh endothelium‐dependent relaxation in BDVs is essentially mediated by nitric oxide and endothelium‐derived prostanoids. The BDV endothelium function is a dynamic component in the control of the bovine digital blood flow, particularly under endothelial dysfunction conditions when venoconstriction might lead to postcapillary resistance increase.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endothelium‐dependent relaxation mechanisms involve nitric oxide and prostanoids in the isolated bovine digital vein

Comerma-Steffensen

Risso

Ascanio-Evanoff

et al. 2019

Vet Pharm & Therapeutics

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“…The neuro‐humoral systems participating in the physiology and pathophysiology of digital vessel tone regulation have been extensively characterized in the horse (Bailey, Marr, & Elliott, ; Katz & Bailey, ). By contrast, the systems operating in the digital vessels of cattle have been investigated only in a limited number of studies (Belloli, Badino, Arioli, Odore, & Re, ; Comerma et al., ; Elmes & Eyre, ; Punzi, Belloli, Gogny, Desfontis, & Mallem, ; Risso et al., ).…”

Section: Introductionmentioning

confidence: 99%

A comparative study between responses of isolated bovine and equine digital arteries to vasoactive mediators

Zizzadoro

Caruso

Punzi

et al. 2017

Vet Pharm & Therapeutics

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Hemodynamic perturbations, partly resulting from abnormal vasoconstriction of digital vessels, have been implicated in the pathogenesis of bovine and equine laminitis.This study compared the responsiveness of isolated bovine (BDA) and equine (EDA) digital arteries to pharmacological agents that stimulate receptor systems involved in the regulation of normal vessel tone. The role of the endothelium and the short-and longer-term effects of an experimentally induced endothelial damage were also evaluated. Species-related differences were found in the vessel reactivity to all of the receptor agonists tested. In intact BDA, as compared to intact EDA, norepinephrine was a more effective vasoconstrictor, 5-hydroxytryptamine a more effective but less potent vasoconstrictor, isoproterenol a less effective vasodilator and carbamylcholine a less potent vasodilator. In BDA, but not in EDA, the contractile responses to norepinephrine and 5-hydroxytryptamine were enhanced immediately after endothelium removal. However, the contractile reactivity of denuded BDA returned to basal values following overnight incubation. The differences suggest species specificity for the pathophysiology of digital vasomotor tone and function in horses and cattle.

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Effect of hypoxia/reoxygenation on the contractility of the isolated bovine digital vein

Cited by 2 publications

References 26 publications

Endothelium‐dependent relaxation mechanisms involve nitric oxide and prostanoids in the isolated bovine digital vein

Endothelium‐dependent relaxation mechanisms involve nitric oxide and prostanoids in the isolated bovine digital vein

A comparative study between responses of isolated bovine and equine digital arteries to vasoactive mediators

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