Effect of Hypoxia on Gene Expression in Cell Populations Involved in Wound Healing

D’Alessandro, Sarah; Magnavacca, Andrea; Perego, Federica; Fumagalli, Marco; Sangiovanni, Enrico; Prato, Maurizio; Dell’Agli, Mario; Parapini, S.

doi:10.1155/2019/2626374

Cited by 25 publications

(25 citation statements)

References 115 publications

(118 reference statements)

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“…The C t cut-off was set to 35. Expression data were normalized to the housekeeping gene RPLP0 (ribosomal protein lateral stalk subunit P0) and elaborated using the ∆∆C t method based on Pfaffl equation [62].…”

Section: Qpcrmentioning

confidence: 99%

Ribes nigrum Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes

et al. 2021

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Ribes nigrum L. (blackcurrant) leaf extracts, due to high levels of flavonols and anthocyanins, have been shown to exhibit beneficial effects in inflammatory diseases. However, whereas their traditional use has been investigated and validated in several models of inflammation and oxidative stress, the possible impact on skin disorders is still largely unknown. The purpose of this work was to elucidate the effects of R. nigrum leaf extract (RNLE) on keratinocyte-derived inflammatory mediators, elicited by a Th1 or Th2 cytokine milieu. HaCaT cells were challenged with TNF-α, either alone or in combination with the costimulatory cytokines IFN-γ or IL-4, and the release of proinflammatory cytokines and mediators (IL-8, IL-6, s-ICAM-1, and TSLP) was evaluated. The results showed that RNLE preferentially interferes with IFN-γ signaling, demonstrating only negligible activity on TNF-α or IL-4. This effect was attributed to flavonols, which might also account for the ability of RNLE to impair TNF-α/IL-4-induced TSLP release in a cAMP-independent manner. These results suggest that RNLE could have an antiallergic effect mediated in keratinocytes via mechanisms beyond histamine involvement. In conclusion, the discovery of RNLE preferential activity against IFN-γ-mediated inflammation suggests potential selectivity against Th1 type response and the possible use in Th1 inflammatory diseases.

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Section: Qpcrmentioning

confidence: 99%

Ribes nigrum Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes

et al. 2021

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“…Many CAs are detected in normal skin [5][6][7], and we showed earlier that the expression of Car4 and Car9 mRNAs are elevated in murine skin wounds compared to normal skin during the hypoxic phase of wound healing [8]. This is thought to be associated with the lack of oxygen in the wound milieu [8,9]. Furthermore, topically administered recombinant CA IV accelerates wound closure [8].…”

Section: Introductionmentioning

confidence: 93%

Carbonic Anhydrase VI in Skin Wound Healing Study on Car6 Knockout Mice

Pemmari

Laakso

Patrikainen

et al. 2020

IJMS

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Carbonic anhydrases (CAs) contribute to tumor cell migration by generating an acidic environment through the conversion of carbon dioxide to bicarbonate and a proton. CA VI is secreted to milk and saliva, and it could contribute to wound closure, as a potential trophic factor, in animals that typically lick their wounds. Our aim was to investigate whether human CA VI improves skin-wound healing in full-thickness skin-wound models. The effect was studied in Car6 −/− knockout mice and wild type littermates. Half of both mice strains were given topically administered, milk-derived CA VI after wounding and eight hours later. The amount of topically given CA VI exceeded the predicted amount of natural saliva-delivered CA VI. The healing was followed for seven days and studied from photographs and histological sections. Our results showed no significant differences between the treatment groups in wound closure, re-epithelization, or granulation tissue formation, nor did the Car6 genotype affect the healing. Our results demonstrate that CA VI does not play a major role in skin-wound healing and also suggest that saliva-derived CA VI is not responsible for the licking-associated improved wound healing in animals.

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“…study showed that the occlusive environment created by the interactive dressing not only decreases the risk of bacterial infection but also provides a moist environment that increases regeneration. [ 37 ]…”

Section: Evolutionary Trend Of Wound Dressingsmentioning

confidence: 99%

Wound Healing: From Passive to Smart Dressings

Farahani

Shafiee

2021

Adv Healthcare Materials

374

219

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The universal increase in the number of patients with nonhealing skin wounds imposes a huge social and economic burden on the patients and healthcare systems. Although, the application of traditional wound dressings contributes to an effective wound healing outcome, yet, the complexity of the healing process remains a major health challenge. Recent advances in materials and fabrication technologies have led to the fabrication of dressings that provide proper conditions for effective wound healing. The 3D‐printed wound dressings, biomolecule‐loaded dressings, as well as smart and flexible bandages are among the recent alternatives that have been developed to accelerate wound healing. Additionally, the new generation of wound dressings contains a variety of microelectronic sensors for real‐time monitoring of the wound environment and is able to apply required actions to support the healing progress. Moreover, advances in manufacturing flexible microelectronic sensors enable the development of the next generation of wound dressing substrates, known as electronic skin, for real‐time monitoring of the whole physiochemical markers in the wound environment in a single platform. The current study reviews the importance of smart wound dressings as an emerging strategy for wound care management and highlights different types of smart dressings for promoting the wound healing process.

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Effect of Hypoxia on Gene Expression in Cell Populations Involved in Wound Healing

Cited by 25 publications

References 115 publications

Ribes nigrum Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes

Ribes nigrum Leaf Extract Preferentially Inhibits IFN-γ-Mediated Inflammation in HaCaT Keratinocytes

Carbonic Anhydrase VI in Skin Wound Healing Study on Car6 Knockout Mice

Wound Healing: From Passive to Smart Dressings

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