Effect of hypoxia and glucose deprivation on ATP level, adenylate energy charge and [Ca2+]0-dependent and independent release of [3H]dopamine in rat striatal slices

Milusheva, E.; Dóda, M; Baranyi, Mária; Vizi, E.S.

doi:10.1016/0197-0186(95)00129-8

Cited by 70 publications

(30 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 3 H]DA release experiments from slices After the 1 h washout period, the basal [ 3 H]DA efflux amounted to 0.551 Ϯ 0.040 FR% (n ϭ 10), similar to that found in our previous study (Milusheva et al, 1996). The same electrical field stimulation parameters were used as to evoke [ 3 H]GABA release.…”

supporting

confidence: 53%

See 1 more Smart Citation

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Köfalvi¹,

Rodrigues²,

Ledent³

et al. 2005

J. Neurosci.

219

194

View full text Add to dashboard Cite

supporting

confidence: 53%

“…The experiments were performed as described previously (Milusheva et al, 1996;Köfalvi et al, 2000), with slight modifications. Briefly, slices were incubated for 30 min at 37°C in the presence of After termination of the 1 h presuperfusion period, 3 min samples were collected for tritium assay (sample collection period).…”

Section: Release Experimentsmentioning

confidence: 99%

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Köfalvi¹,

Rodrigues²,

Ledent³

et al. 2005

J. Neurosci.

219

194

View full text Add to dashboard Cite

“…As seen in this and many other reports (McIlwain, 1952;Thomas, 1957;Kass and Lipton, 1982;Fredholm et al, 1984;Whittingham et al, 1989;Paschen and Djuricic, 1995;Milusheva et al, 1996), brain slices have ϳ40 -60% lower ATP and TAN levels than the in vivo brain (Supplemental References). Our results suggest that this is attributable to the loss of adenine nucleotides and/or their metabolites, especially in hippocampal tissue, during slice preparation.…”

Section: Temperature Dependence Of the Atp/amp Ratio And Ampk Activitymentioning

confidence: 50%

Intracellular ATP Influences Synaptic Plasticity in Area CA1 of Rat Hippocampus via Metabolism to Adenosine and Activity-Dependent Activation of Adenosine A₁Receptors

Nedden¹,

Hawley²,

Pentland³

et al. 2011

J. Neurosci.

View full text Add to dashboard Cite

The extent to which brain slices reflect the energetic status of the in vivo brain has been a subject of debate. We addressed this issue to investigate the recovery of energetic parameters and adenine nucleotides in rat hippocampal slices and the influence this has on synaptic transmission and plasticity. We show that, although adenine nucleotide levels recover appreciably within 10 min of incubation, it takes 3 h for a full recovery of the energy charge (to Ն0.93) and that incubation of brain slices at 34°C results in a significantly higher ATP/AMP ratio and a threefold lower activity of AMP-activated protein kinase compared with slices incubated at room temperature. Supplementation of artificial CSF with D-ribose and adenine (Rib/Ade) increased the total adenine nucleotide pool of brain slices, which, when corrected for the influence of the dead cut edges, closely approached in vivo values. Rib/Ade did not affect basal synaptic transmission or paired-pulse facilitation but did inhibit long-term potentiation (LTP) induced by tetanic or weak theta-burst stimulation. This decrease in LTP was reversed by strong theta-burst stimulation or antagonizing the inhibitory adenosine A 1 receptor suggesting that the elevated tissue ATP levels had resulted in greater activity-dependent adenosine release during LTP induction. This was confirmed by direct measurement of adenosine release with adenosine biosensors. These observations provide new insight into the recovery of adenine nucleotides after slice preparation, the sources of loss of such compounds in brain slices, the means by which to restore them, and the functional consequences of doing so.

show abstract

“…It is noteworthy that the levels of ATP measured in the brain cortex under in vivo conditions (Hisanaga et al 1986;Onodera et al 1986) were considerably higher than those obtained in slice preparations. In hippocampal (Paschen and Djuricic 1995) and neocortical slices (present study), ATP levels were slightly lower than in striatal slices (Milusheva et al 1996). Finally, the ATP content determined in cultured neuronal cells has been reported to exceed these values two-to threefold (Mills et al 1995) and even higher values were found in cortical astrocytes (Fitzpatrick et al 1988).…”

Section: Discussionmentioning

confidence: 80%

Changes by short-term hypoxia in the membrane properties of pyramidal cells and the levels of purine and pyrimidine nucleotides in slices of rat neocortex; effects of agonists and antagonists of ATP-dependent potassium channels

Pissarek

Arriba

Schäfer

et al. 1998

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

In a first series of experiments, intracellular recordings were made from pyramidal cells in layers II-III of the rat primary somatosensory cortex. Superfusion of the brain slice preparations with hypoxic medium (replacement of 95%O2-5%CO2 with 95%N2-5%CO2) for up to 30 min led to a time-dependent depolarization (HD) without a major change in input resistance. Short periods of hypoxia (5 min) induced reproducible depolarizations which were concentration-dependently depressed by an agonist of ATP-dependent potassium (K(ATP)) channels, diazoxide (3-300 microM). The effect of 30 but not 300 microM diazoxide was reversed by washout. Tolbutamide (300 microM), an antagonist of K(ATP) channels, did not alter the HD when given alone. It did, however, abolish the inhibitory effect of diazoxide (30 microM) on the HD. Neither diazoxide (3-300 microM) nor tolbutamide (300 microM) influenced the membrane potential or the apparent input resistance of the neocortical pyramidal cells. Current-voltage (I-V) curves constructed at a membrane potential of -90 mV by injecting both de- and hyperpolarizing current pulses were not altered by diazoxide (30 microM) or tolbutamide (300 microM). Moreover, normoxic and hypoxic I-V curves did not cross each other, excluding a reversal of the HD at any membrane potential between -130 and -50 mV. The hypoxia-induced change of the I-V relation was the same both in the absence and presence of tolbutamide (300 microM). In a second series of experiments, nucleoside di- and triphosphates separated with anion exchange HPLC were measured in the neocortical slices. After 5 min of hypoxia, levels of nucleoside triphosphates declined by 29% (GTP), 34% (ATP), 44% (UTP) and 58% (CTP). By contrast, the levels of nucleoside diphosphates either did not change (UDP) or increased by 13% (GDP) and 40% (ADP). In slices subjected to 30 min of hypoxia the triphosphate levels continued to decrease, while the levels of GDP and ADP returned to control values. The tri- to diphosphate ratios progressively declined for ATP/ADP and GTP/GDP, but not for UTP/UDP when the duration of hypoxia was increased from 5 to 30 min. Hence, the rapid fall in the ratios of nucleoside tri- to diphosphates without the induction of a potassium current failed to indicate an allosteric regulation of a plasmalemmal K(ATP) channel by purine and pyrimidine nucleotides. Diazoxide had no effect on neocortical pyramidal neurons and was effective only in combination with a hypoxic stimulus; it is suggested that both plasmalemmal and mitochondrial K(ATP) channels are involved under these conditions. The hypoxic depolarization may be due to blockade of K+,Na+-ATPase by limitation of energy supplying substrate.

show abstract

Effect of hypoxia and glucose deprivation on ATP level, adenylate energy charge and [Ca2+]0-dependent and independent release of [3H]dopamine in rat striatal slices

Cited by 70 publications

References 41 publications

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Intracellular ATP Influences Synaptic Plasticity in Area CA1 of Rat Hippocampus via Metabolism to Adenosine and Activity-Dependent Activation of Adenosine A₁Receptors

Changes by short-term hypoxia in the membrane properties of pyramidal cells and the levels of purine and pyrimidine nucleotides in slices of rat neocortex; effects of agonists and antagonists of ATP-dependent potassium channels

Contact Info

Product

Resources

About

Effect of hypoxia and glucose deprivation on ATP level, adenylate energy charge and [Ca2+]0-dependent and independent release of [3H]dopamine in rat striatal slices

Cited by 70 publications

References 41 publications

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis

Intracellular ATP Influences Synaptic Plasticity in Area CA1 of Rat Hippocampus via Metabolism to Adenosine and Activity-Dependent Activation of Adenosine A1Receptors

Changes by short-term hypoxia in the membrane properties of pyramidal cells and the levels of purine and pyrimidine nucleotides in slices of rat neocortex; effects of agonists and antagonists of ATP-dependent potassium channels

Contact Info

Product

Resources

About

Intracellular ATP Influences Synaptic Plasticity in Area CA1 of Rat Hippocampus via Metabolism to Adenosine and Activity-Dependent Activation of Adenosine A₁Receptors