Effect of Hypotonic 0.18% Sodium Hyaluronate Eyedrops on Inflammation of the Ocular Surface in Experimental Dry Eye

Oh, Han Jin; Li, Zhengri; Park, Soo-Hyun; Yoon, Kyung Chul

doi:10.1089/jop.2013.0050

Cited by 23 publications

(17 citation statements)

References 44 publications

(61 reference statements)

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“…Both IL-1b and IL-6 are proinflammatory cytokines that are secreted on the ocular surface in response to various pathogenic stimuli, such as benzalkonium chloride-induced EDE, desiccant stressinduced EDE, and microbial infections, such as Pseudomonas aeruginosa, Onchocerca volvulus, and herpes simplex virus 1. 35,48,49 Interleukin-6, along with other proinflammatory cytokines, has a major role in the pathogenesis of several inflammatory diseases, including DED, graft-versus-host disease, and thyroid-associated ophthalmopathy through the NFjB and MAPK pathways. 50,51 In addition, previous studies have shown that increased expression of IL-1b during ocular surface inflammation induces the loss of corneal epithelial barrier function.…”

Section: Discussionmentioning

confidence: 99%

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Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Lee HS, Choi J-H, Cui L, et al. Anti-inflammatory and antioxidative effects of Camellia japonica on human corneal epithelial cells and experimental dry eye: in vivo and in vitro study. Invest Ophthalmol Vis Sci. 2017;58:119658: -120758: . DOI:10.1167 PURPOSE. To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). METHODS.Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE cells. Dichlorofluorescein diacetate (DCF-DA) and dihydroethidium (DHE) assays were performed. The production of peroxiredoxin (PRX) 1-6 and manganesedependent superoxide dismutase (MnSOD) in HCE cells was assessed using Western blot analysis. Furthermore, eye drops containing 0.001%, 0.01%, or 0.1% CJ extract or a balanced salt solution (BSS) were applied to the EDE. Clinical parameters were measured 7 days after treatment. The levels of inflammatory markers and intracellular reactive oxygen species (ROS) were measured.RESULTS. Treatment with 0.01% and 0.1% CJ extracts decreased apoptosis in HCE cells. In addition, band intensities of PRX 1, 4, and 5, as well as MnSOD, after hydrogen peroxide (H 2 O 2 ) treatment showed a significant improvement after pretreatment with 0.01% and 0.1% CJ extracts. Mice treated with 0.1% CJ extract showed significantly improved clinical parameters when compared to those of the EDE control and BSS groups. A significant decrease in the levels of inflammatory markers and intracellular ROS was observed in the 0.01% and 0.1% CJ extract groups.CONCLUSIONS. Camellia japonica extracts promoted antioxidative protein expression and suppressed apoptosis in HCE cells. Furthermore, CJ extracts improved clinical signs of dry eye and reduced oxidative stress and the expression of inflammatory markers, suggesting that eye drops containing CJ extract could be used as an adjunctive treatment for dry eye.

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Section: Discussionmentioning

confidence: 99%

“…35 Briefly, the conjunctival tissues were collected (five eyes per group), pooled in a lysis buffer containing protease inhibitors, and incubated for 30 minutes. The cell extracts then were centrifuged at 14,000g at 48C for 15 minutes, and the supernatants were stored at À708C until use.…”

Section: Multiplex Immunobead Assay For Inflammatory Cytokines and Chmentioning

confidence: 99%

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Lee

Choi

Cui

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“… 9 In addition, HA has been shown to modulate the inflammatory response. 10 More recently, osmoprotectants erythritol, L-carnitine and glycerine have been added to CMC 11 to reduce the cellular stress level of the ocular surface, 12 , 13 but to date, these ingredients have not been combined with HA.…”

Section: Introductionmentioning

confidence: 99%

Osmoprotectants, carboxymethylcellulose and hyaluronic acid multi-ingredient eye drop: a randomised controlled trial in moderate to severe dry eye

Labétoulle

Chiambaretta

Shirlaw

et al. 2017

Eye

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PurposeTo assess the safety and efficacy of an eye drop combining osmoprotectants, carboxymethylcellulose and hyaluronic acid (O/CMC/HA) in reducing symptomatic, moderate to severe dry eye, compared with HA.MethodsIn this investigator-masked, randomised study, patients instilled 1–2 drops/eye of O/CMC/HA or HA (2–6 times/day) for 3 months. Primary endpoint: mean change in Global Ocular Staining Score (GOSS) from baseline at day 35. Noninferiority of O/CMC/HA was tested in the per-protocol population; if achieved, superiority was tested in the intent-to-treat population. Secondary efficacy endpoints: mean change from baseline in GOSS, Ocular Surface Disease Index (OSDI), Schirmer score, tear break-up time (TBUT), corneal/conjunctival staining, conjunctival hyperaemia, symptoms, and patient/investigator assessments.ResultsBaseline characteristics were comparable between groups (n=40 each). O/CMC/HA was noninferior (and not superior) to HA based on similar GOSS reductions from baseline at day 35 and month 3 in both groups (P=0.778, day 35, per-protocol population). Overall, O/CMC/HA and HA provided similar reductions in OSDI, Schirmer score, TBUT, corneal staining and hyperaemia from baseline at 35 days (P≥0.155). More patients reported less severe stinging/burning, sandiness/grittiness, and painful/sore eyes at month 3 with O/CMC/HA (P≤0.039), and more rated the dropper bottle easy to use (87.5%), compared with HA (46.2% P=0.002). Other patient and investigator assessments were similar between groups. O/CMC/HA and HA were well tolerated.ConclusionsO/CMC/HA is noninferior to HA in improving objective signs of dry eye, with potential advantages for subjective symptoms and patient acceptance.

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“…The goblet size density in conjunctiva was high on administration of 0.3% HA and 0.18%HA compared to 0.1% HA. The inflammation on the ocular surfaces decreases with increase in the goblet size [28]. Thus 0.3% HA was most effective when compared to 0.18% and 0.1% HA in the treatment of dry eye disease.…”

Section: Thermosensitive Atelocollagen Punctal Plugmentioning

confidence: 96%

“…Hyaluronic acid based eye drops are coated around the corneal endothelium since it offers a lubricating effect and also offers resistance to dehydration, effectively binds to water and has good biocompatibility [18,19]. Artificial tear eye drops of HA can be used to stabilize the preocular tear film, improve the irregularity on the ocular surface and reduce the symptoms of dry eye [20][21][22]. A study was done to compare the efficacy of preservative free 0.1% HA, 0.18% HA and 0.3% HA artificial tear drops in the mouse model withering stress induced environmental and pharmacological experiment in dry eye disease.…”

Section: Hyaluronic Acid Based Eye Dropsmentioning

confidence: 99%

Recent Trends in Management of Keratoconjunctivitis Sicca (Dry Eye Disease)

et al. 2019

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At the air-water interface, the tear film lipid layer (TFLL), a combination of lipids and proteins plays an important role in surface tension of the tear and is necessary for the physiological hydration of the ocular surface and maintenance of ocular homeostasis. Alteration in lacrimal fluid rheology, differences in lipid constitution or down regulation of particular tear proteins are found in maximum types of ocular surface disease including dry eye disease (DED). Dry eye is a disorder of the tear film due to tear deficiency or excessive tear evaporation, which causes damage to the interpalpebral ocular surface and is associated with symptoms of discomfort. It results in changes on the ocular surface epithelia causing reduced tear quantity and surface sensitivity which leads to inflammation reactions. Managing this inflammation is very helpful in dry eye disease patients. In this article we revise the current understanding of tear film properties, ocular surface and review the effectiveness of topically applied tear supplements, thermo sensitive atelocollagen punctal plug, subtrasal ultrasonic transducers, novel liposome based gelling tear formation and insulin based ophthalmic delivery systems which help in restoring the healthy tear film.

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Effect of Hypotonic 0.18% Sodium Hyaluronate Eyedrops on Inflammation of the Ocular Surface in Experimental Dry Eye

Abstract: Hypotonic 0.18% SH eyedrops are more effective in improving ocular surface irregularity and staining and decreasing inflammatory cytokines, chemokines, and cells on the ocular surface compared with isotonic 0.5% CMC or 0.1% SH eyedrops in the treatment of EDE.

Cited by 23 publications

References 44 publications

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Anti-Inflammatory and Antioxidative Effects of Camellia japonica on Human Corneal Epithelial Cells and Experimental Dry Eye: In Vivo and In Vitro Study

Osmoprotectants, carboxymethylcellulose and hyaluronic acid multi-ingredient eye drop: a randomised controlled trial in moderate to severe dry eye

Recent Trends in Management of Keratoconjunctivitis Sicca (Dry Eye Disease)

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