Effect of Hypertonic Sodium Chloride on Polyribosomes and Protein Synthesis of Kidneys of Rats

Abstract

The effects of a single intraperitoneal administration of hypertonic NaCl(5.3 ml of 7.55% NaCYlOO g body wt) on polyribosomes and in vitro protein synthesis of kidneys of rats were investigated. The results revealed that there was marked disaggregation of kidney polyribosomes of rat (fasted or nonfasted) that received a single dose of hypertonic NaCl solution 30 min prior to sacrifice in comparison to control rats that received isotonic NaCl solution. In vitro [ 14C]leucine incorporation into protein was significantly decreased using either postmitochondrial supernatant or microsomes from kidneys of hypertonic NaCltreated rats compared with control rats. Administration of a single dose of hypertonic NaCl to rats produced a significant increase in the activity of renal RNase in comparison to that of the control group. Assay of renal acid phosphatase in the particulate and soluble fractions revealed no changes in the activities in the control and experimental rats. 258