Effect of Hypertonic Saline Dextran Solution on Human Neutrophil Activation.

Human Polymorphonuclear Cell Death after Exposure to Resuscitation Fluids In Vitro

“…We have previously shown that the choice of resuscitation fluid affects production of reactive oxygen species (ROS) by neutrophils 16,21 and their priming. 22 Both increased ROS generation 23 and priming 24 in turn can alter the rate of PMN apoptosis. Therefore, the present study was performed to confirm whether the lifespan of the PMN is altered by exposure to resuscitation fluids.…”

“…11,16,22 The proapoptotic effect of DEX could then involve, or was mediated by, the formation of oxygen-derived free radicals. These reactive oxygen species have the potential to induce apoptosis, and direct addition of peroxynitrite to neutrophils has been shown to results in a rapid induction of apoptotic cell death.…”

Combat Casualty Care Research: From Bench to the Battlefield

“…Natural colloids (albumin), however, did not excite the neutrophils, and exposure to hypertonic saline (HTS) caused suppression of neutrophil functions. Interestingly, when HTS is combined with dextran, the suppressive properties of HTS overcome the stimulatory properties of dextran [26]. Substitution of racemic lactate (D and L‐isomer) with the L‐isomer of lactate, or ketone bodies ( β ‐hydroxybutyrate), can attenuate the neutrophil activation and alter the expression of leukocyte genes known to be involved in inflammation, cell migration, and apoptosis [27].…”