Effect of Hyperbaric Oxygen on Neutrophil Concentration and Pulmonary Sequestration in Reperfusion Injury

Zamboni, William A.; Wong, Howard P.; Stephenson, Linda L.

doi:10.1001/archsurg.1996.01430190078020

Cited by 57 publications

(37 citation statements)

References 19 publications

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“…In a follow-up study, the pulmonary sequestration of leukocytes was not affected by HBO in the reperfusion period after skeletal muscle ischemia [30]. The ischemic tissue volume in this model was however, small, and consequently the degree of pulmonary leukocyte sequestration was minor [30].…”

Section: Discussionmentioning

confidence: 75%

“…In an animal model, HBO treatment has been shown to reduce the local leukocyte-venular adhesion in ischemia-reperfusion of skeletal muscle [8]. In a follow-up study, the pulmonary sequestration of leukocytes was not affected by HBO in the reperfusion period after skeletal muscle ischemia [30]. The ischemic tissue volume in this model was however, small, and consequently the degree of pulmonary leukocyte sequestration was minor [30].…”

Section: Discussionmentioning

confidence: 81%

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Effects of Hyperbaric Oxygen Treatment on Neutrophil Activation and Pulmonary Sequestration in Intestinal Ischemia- Reperfusion in Rats

Tjärnström¹,

Wikström²,

Bagge

et al. 1999

Eur Surg Res

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Treatment with hyperbaric oxygen (HBO) has shown promising results in some models of ischemia, the major effect being a reduction in the local ischemic damage. The present study investigated the effects of HBO treatment on neutrophil activation and leukosequestration during reperfusion following intestinal ischemia in a rat model. The superior mesenteric artery was clamped for 2 h and subsequently reperfused for 90 min. One group of male Sprague-Dawley rats (n = 9) was given HBO and another group (n = 9) served as controls. Prior to ischemia, leukocytes and erythrocytes were separated, radiolabelled with ¹¹¹ln and ⁵¹Cr, respectively, and reinfused. Leukocyte transit factor, the ratio between the mean passage time of leukocytes and erythrocytes was used to quantitate leukosequestration and the fraction of circulating, spontaneously nitroblue tetrazolium (NBT)-reducing neutrophils was used to measure the degree of neutrophil preactivation. HBO treatment reduced the level of leukocyte pooling significantly, especially in the lungs but also, to a minor degree, in the systemic vascular bed. The percentage of NBT-positive cells increased in all animals after reperfusion, but the increase was significantly reduced by HBO treatment. In conclusion, HBO treatment reduces leukosequestration and neutrophil preactivation following intestinal ischemia-reperfusion.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 81%

Effects of Hyperbaric Oxygen Treatment on Neutrophil Activation and Pulmonary Sequestration in Intestinal Ischemia- Reperfusion in Rats

Tjärnström¹,

Wikström²,

Bagge

et al. 1999

Eur Surg Res

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“…These findings suggest that IR by itself causes the increase of MPO activity in liver and lung and that this increase is accentuated by HO pretreatment of the animals. In a review of the literature, we did not find other studies evaluating the role of HO preconditioning and pulmonary alterations resulting from liver IRI, although an experimental study on rats using HO in a muscle I/R model has shown a reduction in the concentration of pulmonary leukocytes when HO was used 15 . The present results are divergent from those reported in two other studies, in which HO preconditioning exerted a protective effect on liver I/R 10,11 .…”

Section: Discussionmentioning

confidence: 81%

Hyperbaric oxygen therapy aggravates liver reperfusion injury in rats

et al. 2008

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Purpose:To evaluate the effects of hyperbaric oxygen (HO) therapy in the protection against liver ischemia/reperfusion injury. Methods: Thirty-two male Wistar rats were divided into four groups of eight animals each: group A -laparotomy and liver manipulation, group B -liver ischemia and reperfusion, group C -HO pretreatment for 60 min followed by liver ischemia and reperfusion, and group D -pretreatment with ambient air at 2.5 absolute atmospheres for 60 min followed by liver ischemia and reperfusion. Plasma was assayed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Intra-arterial blood pressure was monitored continuously. Myeloperoxidase activity in the liver and lung was assessed 30 min after reperfusion. Results: Plasma AST, ALT and LDH increased after reperfusion in all animals. Plasma ALT values and myeloperoxidase activity in the liver parenchyma were higher in HO-pretreated animals than in groups A, B and D. HO had a negative hemodynamic effect during liver reperfusion. Conclusion: Liver preconditioning with hyperbaric oxygen therapy aggravated liver ischemia/reperfusion injury in rats as demonstrated by plasma ALT and liver myeloperoxidase activity. Key words: Reperfusion Injury. Liver. Hyperbaric Oxygenation. Rats. RESUMOObjetivo: Avaliar os efeitos da oxigenoterapia hiperbárica (OH) como método preventivo da lesão de isquemia e reperfusão (LIR) do fígado. Métodos: Trinta e dois ratos machos Wistar foram distribuídos em quatro grupos de oito animais cada: Alaparotomia e manipulação hepática, B -isquemia e reperfusão hepática, C -pré-tratamento com OH por 60 minutos seguido de isquemia e reperfusão hepática e D -pré-tratamento com ar ambiente a 2,5 atmosferas absolutas por 60 minuto e isquemia e reperfusão hepática. Dosagens seriadas de AST, ALT e DHL foram realizadas. A pressão intra arterial foi monitorizada continuamente. O grau de infiltração leucocitária no fígado e pulmões foi inferido pela dosagem de mieloperoxidade tecidual. Resultados: O nível sérico de AST, ALT e DHL aumentou em todos animais. Os animais expostos a OH apresentaram níveis de ALT e infiltração leucocitária hepática maior que os demais. A OH apresentou efeitos hemodinâmicos negativos durante a reperfusão hepática. Conclusão: O pré-condicionamento hepático por oxigenoteraia hiperbárica agrava a lesão de isquemia e reperfusão hepática em ratos. Descritores: Traumatismo por Reperfusão. Fígado. Oxigenação Hiperbárica. Ratos

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“…NF-B can be activated by different initiators, and hypoxemia is one of the causes [8] . Arterial hypoxemia occurs in more than 60% of patients during the first 48 h of an attack, and adult respiratory distress syndrome may occur in up to 20% of patients.…”

mentioning

confidence: 99%

Hyperbaric Oxygen Reduces Inflammatory Response in Acute Pancreatitis by Inhibiting NF-κB Activation

et al. 2009

Eur Surg Res

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Aim: The mechanism of hyperbaric oxygen (HBO) therapy for acute pancreatitis has not been fully clarified yet. The main purpose of this study was to investigate the effect of HBO on nuclear factor κB (NF-κB) activation and the inflammatory response in rats with acute necrotizing pancreatitis (ANP). Methods: A total of 120 male Sprague-Dawley rats were randomly divided into 3 groups (40 in each): control, ANP and ANP + HBO. ANP rat models were established by a retrograde injection of 5% sodium taurocholate into the pancreatic duct. HBO treatment was performed at 2.5-fold absolute atmospheric pressure in 90% oxygen for 1, 3, 5, and 7 h. The activation of NF-κB and its inhibitor IκBα in peripheral blood neutrophilic granulocytes was measured by electrophoretic mobility shift assay and Western blot, respectively. The inflammatory cytokines [interleukin (IL)-2, IL-6, tumor necrosis factor-α (TNF-α), and intercellular adhesion molecule 1] in the blood were measured by enzyme-linked immunosorbent assay. Results: The blood levels of inflammatory cytokines and NF-κB activation were significantly increased in ANP rats compared to control rats, but IκBα activation was suppressed. The levels of the elevated inflammatory cytokines were positively correlated with the changes in NF-κB activation. After HBO treatment, the blood levels of inflammatory cytokines and NF-κB activation were significantly decreased in the ANP + HBO group in a time-dependent manner, but IκBα activation was increased. Conclusion: Our findings suggest that acute pancreatitis is associated with the upregulation of cytokines in blood as well as upregulation of NF-κB levels and downregulation of IκBα activation in peripheral blood neutrophilic granulocytes. In contrast, HBO plays a role in acute pancreatitis treatment by normalizing these changes.

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Effect of Hyperbaric Oxygen on Neutrophil Concentration and Pulmonary Sequestration in Reperfusion Injury

Cited by 57 publications

References 19 publications

Effects of Hyperbaric Oxygen Treatment on Neutrophil Activation and Pulmonary Sequestration in Intestinal Ischemia- Reperfusion in Rats

Effects of Hyperbaric Oxygen Treatment on Neutrophil Activation and Pulmonary Sequestration in Intestinal Ischemia- Reperfusion in Rats

Hyperbaric oxygen therapy aggravates liver reperfusion injury in rats

Hyperbaric Oxygen Reduces Inflammatory Response in Acute Pancreatitis by Inhibiting NF-κB Activation

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