Effect of hydroxyapatite fillers on the mechanical properties and osteogenesis capacity of bio-based polyurethane composite scaffolds

Du, Jingjing; Zuo, Yi; Lin, Lili; Huang, Di; Niu, Ling; Wei, Yan; Wang, Kaiqun; Qing-fu, Lin; Zou, Qin; Li, Yubao

doi:10.1016/j.jmbbm.2018.08.028

Cited by 27 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Du et al reported that the average pore diameter and porosity of PU scaffold with 40% HA are 500 μm and 57%, respectively. 22 However, in the present work, the PU-32HA sample had an average pore diameter of 125 μm and a porosity of 92%. The reason for these significant differences may be attributed to the role of HA in the pore generation during the PU synthesis process.…”

Section: Field Emission Scanning Electron Microscopycontrasting

confidence: 52%

“…In particular, a significant increase was detected for PU-15HA compared to the PU foam; for the composites of higher HA part, only a modest increase of the porosity was observed with increasing HA content. Du et al 22 reported a 7% difference in the porosity of PU sample containing 20% and 0% HA. However, in the present work the porosity difference between PU-15HA and PU foam was 30%.…”

Section: Physical Characterizationmentioning

confidence: 98%

“…), or mixing of HA with PU foam. 22 In this regard, bio-mineralization of PU foams by immersing synthesized PU foams in the simulated body fluid (SBF) solution has been also reported. 23 To the best of our knowledge, investigation of PU-HA scaffold in which HA exists during in-situ polymerization of PU has not been studied previously.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Three dimensional polyurethane/ hydroxyapatite bioactive scaffolds: The role of hydroxyapatite on pore generation

Nasrollah

Najmoddin

Mohammadi

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

Fabrication of a suitable scaffold with highly interconnected and well-distributed pores for cell proliferation and growth in the field of bone tissue engineering is of high importance. In this study, three-dimensional porous polyurethane (PU) scaffolds, with 0, 15, 25, and 32 wt% hydroxyapatite (HA), were fabricated. In this regard, HA was incorporated into PU constituents prior to starting in-situ polymerization of PU. Porosity and density measurement of the scaffolds revealed that higher amount of HA in the scaffolds led to increasing the former and decreasing the latter quantity. The field emission scanning electron microscopy (FESEM) images revealed that by increasing HA content, the pore size showed a descending trend while the number of pores increased. This would be attributed to the type of interactions between HA and PU, and the role of HA in pore formation. Mechanical test revealed that Young's Modulus of the samples was reduced by increasing scaffold porosity caused by the increase of HA content. Bioactivity tests in the simulated body fluid (SBF) showed the ability of scaffolds forming apatite precipitates. MTT assay showed that by increasing HA content, MG63 osteoblast cell viability increased and FESEM images revealed proper attachment of the cells to the scaffold surface.

show abstract

Section: Field Emission Scanning Electron Microscopycontrasting

confidence: 52%

Section: Physical Characterizationmentioning

confidence: 98%

See 1 more Smart Citation

Three dimensional polyurethane/ hydroxyapatite bioactive scaffolds: The role of hydroxyapatite on pore generation

Nasrollah

Najmoddin

Mohammadi

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…The compression properties of control and decellularized carrot-derived scaffolds ( n = 4, Ø = 10 mm, t = 4 mm) were tested by DMA, after immersion in PBS for 1 week (i.e., Δw plateau). A compression load was applied at a rate of 5% min –1 up to 60% strain, as previously reported for bone tissue engineering ( Du et al, 2018 ). The elastic modulus was calculated as the slope in the 0–20% strain range from the obtained σ–ε curves.…”

Section: Methodsmentioning

confidence: 99%

Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Negrini

Toffoletto

Altomare

2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Decellularized tissues are a valid alternative as tissue engineering scaffolds, thanks to the three-dimensional structure that mimics native tissues to be regenerated and the biomimetic microenvironment for cells and tissues growth. Despite decellularized animal tissues have long been used, plant tissue decellularized scaffolds might overcome availability issues, high costs and ethical concerns related to the use of animal sources. The wide range of features covered by different plants offers a unique opportunity for the development of tissue-specific scaffolds, depending on the morphological, physical and mechanical peculiarities of each plant. Herein, three different plant tissues (i.e., apple, carrot, and celery) were decellularized and, according to their peculiar properties (i.e., porosity, mechanical properties), addressed to regeneration of adipose tissue, bone tissue and tendons, respectively. Decellularized apple, carrot and celery maintained their porous structure, with pores ranging from 70 to 420 µm, depending on the plant source, and were stable in PBS at 37 • C up to 7 weeks. Different mechanical properties (i.e., E apple = 4 kPa, E carrot = 43 kPa, E celery = 590 kPa) were measured and no indirect cytotoxic effects were demonstrated in vitro after plants decellularization. After coating with poly-L-lysine, apples supported 3T3-L1 preadipocytes adhesion, proliferation and adipogenic differentiation; carrots supported MC3T3-E1 pre-osteoblasts adhesion, proliferation and osteogenic differentiation; celery supported L929 cells adhesion, proliferation and guided anisotropic cells orientation. The versatile features of decellularized plant tissues and their potential for the regeneration of different tissues are proved in this work.

show abstract

“…In the lack of PCL crystals at higher temperature (Figure 8(b)), as expected, PU2000 still exhibits higher tensile strength due to its high HBI (C = O) . However, higher contents of HA cause that PU2000S-1 and PU2000-I-1 show higher tensile strength than the nanocomposites containing 0.5 wt% HA nanoparticles (Du et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Synthesis, characterization, crystallinity, mechanical properties, and shape memory behavior of polyurethane/hydroxyapatite nanocomposites

Marand

Rezaei

Babaie

et al. 2020

Journal of Intelligent Material Systems and Structures

View full text Add to dashboard Cite

Herein, polycaprolactone diols with diverse molecular weights were synthesized by ring-opening method. Then, polyurethanes were synthesized through two-step pre-polymerization method by polyaddition of hydroxyl and –NCO groups. Afterward, a set of polyurethanes/hydroxyapatite nanocomposites were synthesized through solution casting as well as in situ polycondensation methods. The exact nominal molecular weights of the synthesized polycaprolactones were determined by proton nuclear magnetic resonance (hydrogen-1 nuclear magnetic resonance). Hydrogen bonding index of ester and urethane carbonyl groups (HBI(C = O)) of samples was determined through Fourier-transform infrared spectroscopy. Results showed that the incorporating of the hydroxyapatite nanoparticles has reduced HBI(C = O). X-ray diffraction patterns and differential scanning calorimetry thermographs confirmed the barrierity and nucleation performance of hydroxyapatite nanoparticles, and the variation of phase mixing degree of polyurethane’s hard and soft segments has altered the crystals size and degree of crystalline in polyurethane/hydroxyapatite nanocomposites. Field emission scanning electron microscope images showed that hydroxyapatite nanoparticles have been uniformly dispersed through in situ polymerization method. Mechanical properties were studied in the terms of HBI(C = O), hydroxyapatite nanoparticles content, and degree of crystallinity. Two different programming procedures were used to evaluate shape fixity and recovery ratios of samples at room temperature and 60°C.

show abstract

Effect of hydroxyapatite fillers on the mechanical properties and osteogenesis capacity of bio-based polyurethane composite scaffolds

Cited by 27 publications

References 38 publications

Three dimensional polyurethane/ hydroxyapatite bioactive scaffolds: The role of hydroxyapatite on pore generation

Three dimensional polyurethane/ hydroxyapatite bioactive scaffolds: The role of hydroxyapatite on pore generation

Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Synthesis, characterization, crystallinity, mechanical properties, and shape memory behavior of polyurethane/hydroxyapatite nanocomposites

Contact Info

Product

Resources

About