Effect of hydroxyapatite-coated nanofibrous membrane on the responses of human periodontal ligament fibroblast

Abstract

In this study, hydroxyapatite-coated biopolymer nanofibrous membrane was synthesized by mineralizing the electrospun polycaprolactone nanofiber. The effect of the hydroxyapatite-coated nanofibrous membrane was mainly investigated on the proliferation and differentiation of human periodontal ligament fibroblast, which is necessary for periodontal tissue regeneration. Scanning electron microscopy revealed favorable cell attachment and spreading appearance on 1 d and MTS assay showed increased cell proliferation on the hydroxyapatite-coated nanofiber membrane during 14 d. From 7 to 14 d, alkaline phosphatase activity of the hydroxyapatite-coated nanofiber was significantly increased when compared to that of control group. Hydroxyapatite-coated nanofibrous membrane showed prominent mineral formation on day 14. As a result, it was clarified that the developed hydroxyapatite-coated nanofibrous membrane had favorable effects on the proliferation and differentiation of human periodontal ligament fibroblast and might be a good candidate material for periodontal tissue regeneration.

Mineralization Content Alters Osteogenic Responses of Bone Marrow Stromal Cells on Hydroxyapatite/Polycaprolactone Composite Nanofiber Scaffolds

“…With regards to the modest increase in ALP activity on polymer-mineral composite scaffolds, other studies using different cell populations (hFOB or human MSCs) also found a similar trend of small increases in ALP activity on mineralized scaffolds [17,18,66,67]. Since these studies used different cell populations and a variety of mineralization levels but yielded similar results, this nominal increase in ALP activity through three weeks on synthetic HAp/PCL composite nanofiber scaffolds can be considered an acceptable and expectable result for in vitro studies.…”

Electrospun fibrous web of collagen–apatite precipitated nanocomposite for bone regeneration

“…However, owing to the limitation of the current study, any general conclusions cannot be drawn regarding this, particularly as to the impact of the nanocomposite composition on the expression of ALP by the osteoblastic cells with respect to the pure collagen. Although showed some down-regulation in ALP at day 7, the HA-collagen nanofiber stimulated cells to express ALP at a significant incremental rate at day 14, and this may be beneficial for the later mineralization and maturation [26,27]. At least however, the currently developed nanofibrous web constituted of collagen and HA was proven to support good cell adhesion and growth and to stimulate osteoblastic differentiation, and thus is considered to have some potential to be useful in the bone regeneration field.…”

Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study

“…nHA has been incorporated in PCL by electrospinning in several studies in vitro [ 63 ] and in vivo in a calvarial defect mouse model where association with HA significantly improved bone healing induced by PCL [ 64 ]. The HA-coated PCL membrane has favorable effects on proliferation and differentiation of human periodontal ligament cells and might be a candidate material for periodontal tissue regeneration [ 65 ]. Similarly, the properties of PCL have also been enhanced by the use of silica [ 66 ], and other pretreatments enhancing mineralization would be of interest for bone tissue mineralization and regeneration as demonstrated for cellulose-based porous matrix [ 67 ].…”