Effect of hydrothermal pretreated bamboo lignin on cellulose saccharification for bioethanol production

Yang, Haiyan; Jin, Yan; Shi, Zhengjun; Wang, Dawei; Zhao, Ping; Yang, Jing

doi:10.1016/j.indcrop.2020.112865

Cited by 37 publications

(6 citation statements)

References 33 publications

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“…Yang et al [ 40 ] reported that lignin with higher negative zeta potentials generated greater repulsion of enzymes, resulting in improved enzymatic digestibility by reducing nonproductive binding. Hence, the zeta potentials of different lignin samples (SL and MWL) were also measured to compare their different effects on enzymatic hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

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Section: Resultsmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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“…However, the relatively low solid-liquid ratio decreased the concentration of xylose and glucose (Potumarthi R et al, 2014). Compared with the hydrothermal pretreatment, surfactant-assisted hydrothermal pretreatment achieved the higher cellulose conversion rates at 1:8 solid-liquid ratio (Charnnok B et al, 2020;Yang H et al, 2020). The concentration of furfural and 5-HMF increased and reached maximum on SL6 (Fig.…”

Section: Resultsmentioning

confidence: 97%

Surfactant-assisted hydrothermal pretreatment for co- production of reducing sugars and carbon quantum dots from lignocellulosic biomass

Jiang,

Lu,

Bie

et al. 2023

Preprint

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A facile and environmentally friendly strategy was proposed for co-production of reducing sugars and carbon quantum dots (CQDs) from lignocellulosic biomass (LCB) by one-pot surfactant-assisted hydrothermal pretreatment. Compared with hydrothermal pretreatment, the addition of surfactants (Tween 40) significantly increased the sugars concentration and effective quantum yield (QY). The highest reducing sugars concentration and QY of CQDs was up to 22.47 g/L and 1.9% respectively. After surfactant-assisted pretreatment, the crystallinity index of LCB residue increased and then decreased with the addition amount of Tween 40. Moreover, the conversion rate of cellulose and hemicellulose reached 95.1% and 95.4% respectively, 122.2% and 41.1% higher than that without Tween 40. It was also found that surfactant-assisted hydrothermal pretreatment had the same improvement effect on increasing the sugar production of other LCB. The results showed that surfactant-assisted hydrothermal pretreatment was a promising and universal method for developing biomass-based products.

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“…According to the study by Yang et al, lignin with higher negative zeta potentials showed greater interaction with enzymes, which might be due to the higher electrostatic forces. 54 2D-HSQC and 31 P NMR were performed to study the chemical structure of different lignin fractions in DAP-BR. Lignin has been identified as an aromatic polymer assembled from phenylpropane units, which are linked by ether (C-O-C) and carbon-carbon (C-C) bonds to form a complex phenolic compound.…”

Section: Characterization Of Lignin Fractionsmentioning

confidence: 99%

“…According to the study by Yang et al , lignin with higher negative zeta potentials showed greater interaction with enzymes, which might be due to the higher electrostatic forces. 54…”

Section: Influence Of Milk Protein Pre-adsorption On Physicochemical ...mentioning

confidence: 99%

Evaluating the mechanism of milk protein as an efficient lignin blocker for boosting the enzymatic hydrolysis of lignocellulosic substrates

et al. 2022

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Effect of hydrothermal pretreated bamboo lignin on cellulose saccharification for bioethanol production

Cited by 37 publications

References 33 publications

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Surfactant-assisted hydrothermal pretreatment for co- production of reducing sugars and carbon quantum dots from lignocellulosic biomass

Evaluating the mechanism of milk protein as an efficient lignin blocker for boosting the enzymatic hydrolysis of lignocellulosic substrates

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