Effect of hydrogen ion implantation on cholesterol sensing using enzyme-free LAPONITE®-montmorillonite electrodes

Joshi, Nidhi; Sharma, Abhimanyu; Asokan, K.; Rawat, Kamla; Kanjilal, D.

doi:10.1039/c5ra27523g

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…EIS is an important technique which provides information about the resistance within the electrode material and also the resistance between the electrolyte and the electrode. 41 42,43 After immobilization of antigens over the irradiated electrodes [CagA antigen@ZnO-T/SP-AuE (ϕ = 10 15 ) and CagA antigen@ ZnO-T/SP-AuE (ϕ = 10 16 )] having large diameter showed greater hindrance to electron transfer as compared to the small semicircle of ZnO-T modified SP-electrodes (irradiated). Both CV and EIS are consistent in showing the modification of the electrode by ion irradiation.…”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, after ion beam irradiation with different fluences of N 2 + ions (1 × 10 15 and 1 × 10 16 ) Ret gradually decreases having the lowest Ret of 160 Ω, recorded for ZnO-T/SP-AuE (ϕ = 10 15 ). The modified electrode irradiation with ϕ = 10 15 (low fluence) results in improved charge transfer owing to breakage of H-bonds resulting in creation of active sites enhancing diffusion of Ferricyanide and Ferrocyanide ions toward the surface of modified electrode. , After immobilization of antigens over the irradiated electrodes [CagA antigen@ZnO-T/SP-AuE (ϕ = 10 15 ) and CagA antigen@ZnO-T/SP-AuE (ϕ = 10 16 )] having large diameter showed greater hindrance to electron transfer as compared to the small semicircle of ZnO-T modified SP-electrodes (irradiated). Both CV and EIS are consistent in showing the modification of the electrode by ion irradiation.…”

Section: Resultsmentioning

confidence: 99%

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Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing ofHelicobacter pylori

Chauhan

Gupta

Avasthi

et al. 2018

ACS Appl. Mater. Interfaces

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Helicobacter pylori is a Gram-negative, spiral shaped, microaerophilic bacteria that colonizes human gastric mucosa and causes various gastric diseases. In this work, the utilization of ion irradiated zinc oxide tetrapods (ZnO-T) based biohybrid interface accentuates the development of an electrochemical immunosensor for the fast and sensitive detection of H. pylori. After coating of (ZnO-T) over the surface of screen printed electrode (SP-AuE) through electrodeposition, the ZnO-T/SP-AuE was irradiated with N ion of energy 100 keV. The ion irradiation significantly enhances the conductivity of ZnO-T coated SP-AuE. The revamped SP-AuE is further used for establishing an immunosensor interface based upon immobilization of the CagA antigen on ZnO-T electrodeposited over the surface of SP-AuE. The sensing interface demonstrated good linearity (0.2 ng/mL to 50 ng/mL) and limit of detection (0.2 ng/mL). The ion beam irradiated ZnO-T based immunosensor showed significantly high conductivity and enhanced the analytical properties of the working electrode in terms of the sensitivity, detection limit, and response time. A study on the comparison of irradiated and pristine electrode is performed for amperometric sensing of H. pylori. In addition, the significance of work conducted on ion irradiated ZnO-T based interfaces provides a basis of further development of electrochemical immunosensors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing ofHelicobacter pylori

Chauhan

Gupta

Avasthi

et al. 2018

ACS Appl. Mater. Interfaces

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“…Already published results for non-enzymatic Chol sensors have been quite varied; for instance, Tong et al reported a low linear range of 10-300 nM with an LOD of 1 nM using MWCNT and a molecularly imprinted polymer [40]. Yoshi and coworkers reported a higher linearity range of 1-20 mM (that is, within the range of cholesterol found in blood of 1.81-4.9 mmol/L) but a low sensitivity (8.08 µA mM −1 cm −2 ) using Laponite-Montmorillonite material [41]. As for copper oxide materials, Khaliq et al recently reported Cu 2 O NP/TiO 2 nanotubes with a linear range of 24.4-622 µM, 0.05 µM of LOD, and 6034.04 µA mM −1 cm −2 sensitivity [18].…”

Section: Evaluation Of Cu/cu 2 O As Cholesterol Electrochemical Sensormentioning

confidence: 99%

Copper nanoparticles suitable for bifunctional cholesterol oxidation reaction: harvesting energy and sensor

Espinosa-Lagunes

Cruz

Vega-Azamar

et al. 2022

Mater Renew Sustain Energy

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This study reports the performance of simple low-cost synthesized bifunctional Cu/Cu2O nanoparticles (NPs) used as a catalyst for energy-harvesting applications through of a microfluidic fuel cell (µFC), and further, as cholesterol (Chol) sensor. TEM characterization of the NPs showed spheres between 4 and 10 nm, while XRD and XPS analysis confirmed the composition and preferential crystallographic plane of Cu/Cu2O. In addition, 25.26 m2 g−1 surface area was obtained, which is greater than those commercial materials. NPs showed high activity toward the cholesterol oxidation reaction when were used as a sensor, obtaining a linear interval between 0.5 and 1 mM and 850 µA mM−1 mg−1 of sensitivity and 8.9 µM limit of quantification LOQ. These values are comparable to results previously reported. Moreover, Cu/Cu2O NPs were used as anode in a µFC with 0.96 V of cell voltage and 6.5 mA cm−2 and 1.03 mW cm−2 of current and power density, respectively. This performance is the highest currently reported for cholesterol application as an alternative fuel, and the first one reported for a microfluidic fuel cell system as far as is known. Results showed that the obtained Cu-based NPs presented an excellent performance for the dual application both µFC and sensor, which has potential applications in biomedicine and as an alternative energy source.

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“…Joshi et al studied the effect of hydrogen ion implantation on the synthetic nanoclay electrode for non-enzymatic sensing of cholesterol. The developed LAPONITE ® -montmorillonite/indium tin oxide (L-MMT/ITO) film displays about a 20% increase in sensitivity for cholesterol detection at the ion fluence of ~1013 ions per cm 2 [ 48 ]. The performance of the sensor is illustrated by schematic as shown in Figure 5 .…”

Section: Nanomaterials For Non-enzymatic Cholesterol Biosensormentioning

confidence: 99%

Emerging Trends in Non-Enzymatic Cholesterol Biosensors: Challenges and Advancements

2022

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The development of a highly sensitive and selective non-enzymatic electrochemical biosensor for precise and accurate determination of multiple disease biomarkers has always been challenging and demanding. The synthesis of novel materials has provided opportunities to fabricate dependable biosensors. In this perspective, we have presented and discussed recent challenges and technological advancements in the development of non-enzymatic cholesterol electrochemical biosensors and recent research trends in the utilization of functional nanomaterials. This review gives an insight into the electrochemically active nanomaterials having potential applications in cholesterol biosensing, including metal/metal oxide, mesoporous metal sulfide, conductive polymers, and carbon materials. Moreover, we have discussed the current strategies for the design of electrode material and key challenges for the construction of an efficient cholesterol biosensor. In addition, we have also described the current issues related to sensitivity and selectivity in cholesterol biosensing.

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Effect of hydrogen ion implantation on cholesterol sensing using enzyme-free LAPONITE®-montmorillonite electrodes

Abstract: Electrochemical profiling of LAPONITE®-montmorillonite/indium tin oxide (L-MMT/ITO) electrodes irradiated with 20 keV H2+ ion beam with variable fluence ranging from 1012 to 1016 ions per cm2.

Cited by 10 publications

References 27 publications

Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing ofHelicobacter pylori

Zinc Oxide Tetrapods Based Biohybrid Interface for Voltammetric Sensing ofHelicobacter pylori

Copper nanoparticles suitable for bifunctional cholesterol oxidation reaction: harvesting energy and sensor

Emerging Trends in Non-Enzymatic Cholesterol Biosensors: Challenges and Advancements

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