Effect of hydraulic residence time on biological sulphate reduction and elemental sulphur recovery in a single-stage hybrid linear flow channel reactor

Marais, T.S.; Huddy, Robert J.; Harrison, S T L; Hille, Robert P. van

doi:10.1016/j.bej.2020.107717

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…This was attributed to partial sulphide oxidation at the surface, whereby hydroxyl ions are released during the formation of elemental sulphur. These findings were consistent with previous investigations (Marais et al, 2020a;Marais et al, 2020b;Marais et al, 2020c).…”

Section: Effect Of Temperature On Biological Sulphate Reduction Perfo...supporting

confidence: 94%

“…A 2 L lactate-fed reactor had been operated continuously for 1,137 days since the initial laboratory-scale demonstration (Marais et al, 2020a;Marais et al, 2020b). The 8 L lactate-fed and 2 L acetate-fed reactors were operated for approximately 488 and 524 days prior to the current investigation, respectively (Marais et al, 2020c).…”

Section: Evaluating the Effect Of Temperature On Process Performancementioning

confidence: 99%

“…The harvested sulphur biofilms were collected, dried at 60 °C overnight, prior to elemental analysis. The selection of the 2 day HRT and biofilm disruption interval was informed by previous optimisation experiments (Marais et al, 2020b). These showed that, at 30 °C, complete biofilm recovery occurred within 24 h of disruption and that if the disruption interval was extended beyond 3 HRTs the biofilm became so thick that oxygen mass transfer was impeded to the point where sulphide oxidation became limiting and a significant increase in aqueous sulphide concentration was detected in the effluent.…”

Section: Evaluating the Effect Of Temperature On Process Performancementioning

confidence: 99%

“…The process relies on the formation of niche microenvironments comprising an microaerobic zone at the air-liquid interface and an anoxic zone within the bulk volume of the LFCR. Under ideal conditions the synergistic interaction between sulphatereducing (SRB) and sulphide-oxidising bacterial (SOB) consortia can facilitate a linear biotransformation of sulphate to elemental sulphur (Marais et al, 2020b). In the hybrid LFCR, SRB in the anoxic bulk volume reduce sulphate in the presence of a suitable carbon substrate and electron donor to hydrogen sulphide (HS − ).…”

Section: Introductionmentioning

confidence: 99%

“…Previous investigations have reported on the proof-ofconcept, development and characterisation of the hybrid LFCR process under different operating parameters, such as the effect of hydraulic residence time (HRT), carbon substrate and reactor geometry (Marais et al, 2020a;Marais et al, 2020b;Marais et al, 2020c). These studies have highlighted the potential of the hybrid LFCR as an alternative semi-passive technology, achieving relatively high sulphate and sulphide removal efficiencies with the recovery of elemental sulphur as a valueadding by-product.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics

Marais

Huddy

Hille

et al. 2022

Front. Bioeng. Biotechnol.

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Semi-passive bioremediation is a promising strategy to mitigate persistent low volume mine-impacted wastewater containing high sulphate concentrations. Building on the proof of concept demonstration of the hybrid linear flow channel reactor (LFCR), capable of simultaneous biological sulphate reduction and partial sulphide oxidation with elemental sulphur recovery, the impact of key operating parameters, such as temperature, on process performance is critical to real-world application. Temperature fluctuates seasonally and across the diurnal cycle, impacting biological sulphate reduction (BSR) and partial sulphide oxidation. The process is reliant on the metabolic activity and synergistic interactions between sulphate-reducing (SRB) and sulphide-oxidising (SOB) microbial communities that develop within discrete oxic and anoxic microenvironments within the hybrid LFCR. In this study, the impact of operating temperature on process performance was evaluated by decreasing temperature with time from 30 to 10°C in each of three laboratory-scaled hybrid LFCR units operating in pseudo-steady state at 1 g/L sulphate. Using lactate as a carbon source, two reactor sizes (2 and 8 L) were considered, while the impact of lactate vs. acetate as carbon source was evaluated in the 2 L reactors. On incremental decrease in temperature from 30 to 10°C, a decrease in volumetric sulphate reduction rate was observed: from 0.144 to 0.059 mmol/L.h in the 2 L lactate-fed reactor; from 0.128 to 0.042 mmol/L.h in the 8 L lactate-fed reactor; and from 0.127 to 0.010 mmol/L.h in the 2 L acetate-fed reactor. Similarly, sulphate conversion efficiency decreased (2 L lactate-fed: 66% to 27%; 8 L lactate-fed: 61% to 20%; 2 L acetate-fed: 61% to 5%). A decrease in temperature below the critical value (15°C) led to considerable loss in metabolic activity and overall BSR performance. Sessile and planktonic microbial communities were represented by bacterial phyla including Proteobacteria, Synergistetes, Bacteroidetes, and Firmicutes. A diverse group of putative SRB (Deltaproteobacteria) and SOB, including Alpha, Beta, Gamma, and Epsilonproteobacteria phylotypes, were prevalent and shifted in relative abundance and community composition in response to decreasing temperature. Specifically, the decrease in the relative abundance of Deltaproteobacteria with decreasing temperature below 15°C corresponded with a loss of BSR performance across all three reactors. This study demonstrated the impact of low temperature on the physiological selection and ecological differentiation of SRB and SOB communities within the hybrid LFCR and its implications for real-world process performance.

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Section: Effect Of Temperature On Biological Sulphate Reduction Perfo...supporting

confidence: 94%

Section: Evaluating the Effect Of Temperature On Process Performancementioning

confidence: 99%

Section: Evaluating the Effect Of Temperature On Process Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics

Marais

Huddy

Hille

et al. 2022

Front. Bioeng. Biotechnol.

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Metagenomic analysis of microbial community and metabolic pathway of simultaneous sulfide and nitrite removal process exposed to divergent hydraulic retention times

Kai-quan

Mahmood

Chen

et al. 2022

Bioresource Technology

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Using sulfide as nitrite oxidizing bacteria inhibitor for the successful coupling of partial nitrification-anammox and sulfur autotrophic denitrification in one reactor

Zhao,

Dong,

Chen

et al. 2023

Chemical Engineering Journal

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Effect of hydraulic residence time on biological sulphate reduction and elemental sulphur recovery in a single-stage hybrid linear flow channel reactor

Cited by 10 publications

References 30 publications

Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics

Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics

Metagenomic analysis of microbial community and metabolic pathway of simultaneous sulfide and nitrite removal process exposed to divergent hydraulic retention times

Using sulfide as nitrite oxidizing bacteria inhibitor for the successful coupling of partial nitrification-anammox and sulfur autotrophic denitrification in one reactor

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