Effect of Hybrid Nanomaterial of Copper-Chitosan against Aflatoxigenic Fungi in Poultry Feed

Hassan, Atef A.; Oraby, Noha H.; El-mesalamy, Manal Mohamad; Sayed-ElAhl, Rasha M.H.

doi:10.36380/jwpr.2022.18

Cited by 2 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nanocomposite of low level of ZnONPs (100 μg /ml) with (0.25% Curcumin), and even the elevation of Curcumin concentration to (1%) at the low level of ZnONPs, significantly increased the inhibitory effects against C. albicans and S. aureus, respectively. Similarly, Hassan et al (2019) detected the MIC of ZnONPs against Fusarium spp. was (500 μg/ml) and significantly decreased to (100 μg /ml) when combined with Curcumin or probiotics (0.25% for each).…”

Section: Resultsmentioning

confidence: 99%

“…The antimicrobial potential of ZnONPs detected against bacteria and fungi 2020b & C; that caused skin infection in buffaloes (Hassan et al, 2015) and mastitis in cattle (C. albicans and S. aureus) (Sabir et al, 2014). The antimicrobial potentials of ZnONPs are related to their ability to cause the destruction and death of pathogens cells (Vasilache et al, 2011;Hassan et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…(3,4). The antimicrobial potential of Curcumin due to its ability to inhibit the viability of pathogen cells, such as depressing the protein and DNA synthesis (Anwar et al, 2009;El-Baroty et al, 2010;Hassan et al, 2022) and resulting in genotoxicity (Abd El-Baky and El-Baroty, 2008;and Hassan et al, 2021). Nowadays, there is a continuous awareness of the suspected toxicity of nanotechnology applications in biomedicine.…”

Section: Resultsmentioning

confidence: 99%

“…This allows us to overcome the use of high doses of nanomaterials, otherwise low safe doses in conjugation with natural benefit materials as the combination of ZnONPs with Curcumin and enable to inhibit the viability of virulent genes expression as detected here by PCR. Similarly, the PCR enables the rapid detection of pathogens' genes as recommended by Hu et al (2020), Hassan et al (2021Hassan et al ( , 2022 who found that the use of nanocomposites with natural plant materials caused significant changes in aflatoxin regulatory genes. Other studies illustrated that the traditional antifungals and herbs against C. albicans increased the activity of secreted proteinase (Sap) and SAP genes, which can be observed by PCR (Copping et al 2005;Saleh 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Worldwide progressive attention is made to food safety and human and animal health; hence, animal products are of massive economic significance for human use as milk, meat, and others (Hassan et al, 2020a(Hassan et al, & b, 2021(Hassan et al, , 2022. Several animal diseases are adversely suppressing their health and productivity, particularly dairy animal mastitis, which suppresses milk and meat yields (Hassan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of Zinc Oxide Nano particles and Curcumin effect on some virulence genes of Staphylococcus aureus and Candida albicans causing subclinical mastitis in buffalo

El-Hamaky

Mohamed²,

El-Khalek³

et al. 2023

Egyptian Journal of Agricultural Research

View full text Add to dashboard Cite

Nowadays, there are progressive advancements in the biomedical employment of nanotechnology in human and animal health. Today, the dangers of infectious animal diseases and the continuous application of traditional antibiotics resulted in the elevation of the activities of the drugs resistant genes and the failure of their control. Hence, it is necessary to prevent fungal and bacterial pathogens pollution and their toxicity in dairy animal products. Sixty samples were collected from dairy buffalo farms suffering from subclinical mastitis (20 Samples of each from subclinical mastitis milk, animal feeds, and bedding). The species of Staphylococcus is considered the most predominant species, and Staphylococcus aureus was the most prevalent bacterial isolate. While, yeast, especially Candida albicans, was the most dominant fungal isolate from different samples of dairy buffalo farms. Our antibiogram of S. aureus and Candida albicans showed a high resistance rate against several traditional antimicrobial agents. Therefore, searching for effective drug materials to overcome drug resistance is needed. Herein, ZnONPs and Curcumin are evaluated singly and, in a composite, to inhibit the growth of C. albicans and S. aureus. The inhibitory dose of ZnONPs for C. albicans and S. aureus were (600 and 600 μg /ml) and in Curcumin were (2% and 3%), respectively. However, the hybrid nanocomposite of ZnONPs (100 μg/ml) with Curcumin (0.25% and 1%) caused suppression of the growth of these pathogens. The nanocomposite of low levels of ZnONPs (100 μg /ml) with (0.25% curcumin) significantly increased the inhibitory effects against C. albicans and S. aureus. The molecular study to detect the efficiency of the treatment doses indicated that the exposure of C. albicans and S. aureus to a low dose of ZnONPs (100 µg/ml) did not affect the DNA expression of virulent genes. While the high dose (600 µg/ml) prevented the DNA expression. However, in the Curcumin treatments of isolates with low doses (0.25), the genes' DNA expression was not affected. While, the high dose (1%), removed the DNA band. All inhibition and removal of virulent genes' DNA of pathogens can be obtained by the combination of low levels of ZnONPs and Curcumin. Hence, it is concluded that the nanocomposites of ZnONPs and Curcumin caused significant effects against pathogens.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%